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Efficacy of a mesenchymal stem cell loaded surgical mesh for tendon repair in rats.

Schon LC, Gill N, Thorpe M, Davis J, Nadaud J, Kim J, Molligan J, Zhang Z - J Transl Med (2014)

Bottom Line: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel.A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats.The tendon defects were repaired with either 1) MSC-loaded mesh; or 2) surgical mesh only; or 3) routine surgical suture.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Orthopaedic Surgery, MedStar Union Memorial Hospital, 3333 North Calvert Street, Johnston Professional Building, Suite 400, Baltimore, MD 21218, USA. lewschon@gmail.com.

ABSTRACT

Objectives: The purpose of this study was to investigate the efficacy of a composite surgical mesh for delivery of mesenchymal stem cells (MSCs) in tendon repair.

Methods: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel. A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats. The tendon defects were repaired with either 1) MSC-loaded mesh; or 2) surgical mesh only; or 3) routine surgical suture. Repaired tendons were harvested at days 6 and 14 for histology, which was scored on the bases of collagen organization, vascularity and cellularity, and immunohistochemisty of types I and III collagen.

Results: In comparison with the other two repair types, at day 6, the MSC-loaded mesh significantly improved the quality of the repaired tendons with dense and parallel collagen bundles, reduced vascularity and increased type I collagen. At day 14, the MSC-loaded mesh repaired tendons had better collagen formation and organization.

Conclusion: The MSC-loaded mesh enhanced early tendon healing, particularly the quality of collagen bundles. Application of the MSC-loaded mesh, as a new device and MSC delivery vehicle, may benefit to early functional recovery of the ruptured tendon.

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Related in: MedlinePlus

Quantification of tendon healing. A: The overall scores of the repaired tendons in three study groups. At day 6, tendon repair was significantly improved in group M + S. B: The angiogenesis scores of the repaired tendons. At day 6, angiogenesis in group M + S was significantly less than groups M and Sut. C: The collagen scores of the repaired tendons. The scores of collagen bundles in group M + S showed significant improvement at day 14. D: No differences were found among groups in cellularity score (*p < 0.05; **p < 0.001).
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Figure 3: Quantification of tendon healing. A: The overall scores of the repaired tendons in three study groups. At day 6, tendon repair was significantly improved in group M + S. B: The angiogenesis scores of the repaired tendons. At day 6, angiogenesis in group M + S was significantly less than groups M and Sut. C: The collagen scores of the repaired tendons. The scores of collagen bundles in group M + S showed significant improvement at day 14. D: No differences were found among groups in cellularity score (*p < 0.05; **p < 0.001).

Mentions: At day 6, histology showed that fibroblastic cells filled in the tendon defects in all three groups. Although cell density was high in both M and M + S groups, there was no inflammatory response around PGA fibers. Compared with M and Sut groups, there was more matrix deposition in M + S group (Figure 2) and the matrix appeared in an organized pattern that run in parallel along the tendon (insert). The modified tendon repair score of M + S group was significantly improved over the M and Sut groups (p < 0.05; Figure 3A). When the scores were further analyzed at subcategories, it appeared that reduced vascularity in M + S group contributed most to the overall quality of the repaired tendon in M + S group (Figure 3B).


Efficacy of a mesenchymal stem cell loaded surgical mesh for tendon repair in rats.

Schon LC, Gill N, Thorpe M, Davis J, Nadaud J, Kim J, Molligan J, Zhang Z - J Transl Med (2014)

Quantification of tendon healing. A: The overall scores of the repaired tendons in three study groups. At day 6, tendon repair was significantly improved in group M + S. B: The angiogenesis scores of the repaired tendons. At day 6, angiogenesis in group M + S was significantly less than groups M and Sut. C: The collagen scores of the repaired tendons. The scores of collagen bundles in group M + S showed significant improvement at day 14. D: No differences were found among groups in cellularity score (*p < 0.05; **p < 0.001).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4020316&req=5

Figure 3: Quantification of tendon healing. A: The overall scores of the repaired tendons in three study groups. At day 6, tendon repair was significantly improved in group M + S. B: The angiogenesis scores of the repaired tendons. At day 6, angiogenesis in group M + S was significantly less than groups M and Sut. C: The collagen scores of the repaired tendons. The scores of collagen bundles in group M + S showed significant improvement at day 14. D: No differences were found among groups in cellularity score (*p < 0.05; **p < 0.001).
Mentions: At day 6, histology showed that fibroblastic cells filled in the tendon defects in all three groups. Although cell density was high in both M and M + S groups, there was no inflammatory response around PGA fibers. Compared with M and Sut groups, there was more matrix deposition in M + S group (Figure 2) and the matrix appeared in an organized pattern that run in parallel along the tendon (insert). The modified tendon repair score of M + S group was significantly improved over the M and Sut groups (p < 0.05; Figure 3A). When the scores were further analyzed at subcategories, it appeared that reduced vascularity in M + S group contributed most to the overall quality of the repaired tendon in M + S group (Figure 3B).

Bottom Line: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel.A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats.The tendon defects were repaired with either 1) MSC-loaded mesh; or 2) surgical mesh only; or 3) routine surgical suture.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Orthopaedic Surgery, MedStar Union Memorial Hospital, 3333 North Calvert Street, Johnston Professional Building, Suite 400, Baltimore, MD 21218, USA. lewschon@gmail.com.

ABSTRACT

Objectives: The purpose of this study was to investigate the efficacy of a composite surgical mesh for delivery of mesenchymal stem cells (MSCs) in tendon repair.

Methods: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel. A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats. The tendon defects were repaired with either 1) MSC-loaded mesh; or 2) surgical mesh only; or 3) routine surgical suture. Repaired tendons were harvested at days 6 and 14 for histology, which was scored on the bases of collagen organization, vascularity and cellularity, and immunohistochemisty of types I and III collagen.

Results: In comparison with the other two repair types, at day 6, the MSC-loaded mesh significantly improved the quality of the repaired tendons with dense and parallel collagen bundles, reduced vascularity and increased type I collagen. At day 14, the MSC-loaded mesh repaired tendons had better collagen formation and organization.

Conclusion: The MSC-loaded mesh enhanced early tendon healing, particularly the quality of collagen bundles. Application of the MSC-loaded mesh, as a new device and MSC delivery vehicle, may benefit to early functional recovery of the ruptured tendon.

Show MeSH
Related in: MedlinePlus