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Efficacy of a mesenchymal stem cell loaded surgical mesh for tendon repair in rats.

Schon LC, Gill N, Thorpe M, Davis J, Nadaud J, Kim J, Molligan J, Zhang Z - J Transl Med (2014)

Bottom Line: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel.A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats.Application of the MSC-loaded mesh, as a new device and MSC delivery vehicle, may benefit to early functional recovery of the ruptured tendon.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Orthopaedic Surgery, MedStar Union Memorial Hospital, 3333 North Calvert Street, Johnston Professional Building, Suite 400, Baltimore, MD 21218, USA. lewschon@gmail.com.

ABSTRACT

Objectives: The purpose of this study was to investigate the efficacy of a composite surgical mesh for delivery of mesenchymal stem cells (MSCs) in tendon repair.

Methods: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel. A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats. The tendon defects were repaired with either 1) MSC-loaded mesh; or 2) surgical mesh only; or 3) routine surgical suture. Repaired tendons were harvested at days 6 and 14 for histology, which was scored on the bases of collagen organization, vascularity and cellularity, and immunohistochemisty of types I and III collagen.

Results: In comparison with the other two repair types, at day 6, the MSC-loaded mesh significantly improved the quality of the repaired tendons with dense and parallel collagen bundles, reduced vascularity and increased type I collagen. At day 14, the MSC-loaded mesh repaired tendons had better collagen formation and organization.

Conclusion: The MSC-loaded mesh enhanced early tendon healing, particularly the quality of collagen bundles. Application of the MSC-loaded mesh, as a new device and MSC delivery vehicle, may benefit to early functional recovery of the ruptured tendon.

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Related in: MedlinePlus

Design and application of MSC-loaded surgical mesh. A: Composition of MSC-loading mesh. B: Distribution of MSCs in the PGA-based scaffold. MSCs (green) were labeled with fluorescent dye SYTO®10. PGA fibers also showed green in auto-fluorescence (bar = 50 μm). C: The MSC-loaded mesh was used to repair the 3-mm defect (between the dot lines) created at the junction of Achilles tendon and gastrocnemius/soleus. The portion of scaffold/MSCs was inserted into the defect and surgical mesh was sutured to the surface of muscle and tendon (black arrowhead indicates a knot of surgical suture used to tie the scaffold and surgical mesh together. The suture was melted after autoclave).
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Figure 1: Design and application of MSC-loaded surgical mesh. A: Composition of MSC-loading mesh. B: Distribution of MSCs in the PGA-based scaffold. MSCs (green) were labeled with fluorescent dye SYTO®10. PGA fibers also showed green in auto-fluorescence (bar = 50 μm). C: The MSC-loaded mesh was used to repair the 3-mm defect (between the dot lines) created at the junction of Achilles tendon and gastrocnemius/soleus. The portion of scaffold/MSCs was inserted into the defect and surgical mesh was sutured to the surface of muscle and tendon (black arrowhead indicates a knot of surgical suture used to tie the scaffold and surgical mesh together. The suture was melted after autoclave).

Mentions: The second component of the MSC-loaded mesh was a layer of non-woven scaffold, 4 x 4 x 2 mm3, made of polyglycolic acid (PGA, 60 mg/ml in density, Lot # 10-001253A-1015, Biomedical Structures). The scaffold was attached to the center of the surgical mesh with one knot using a 4–0 monofilament suture. The purpose of the PGA scaffold was to support and attach the hydrogel component to the mesh (Figure 1A).


Efficacy of a mesenchymal stem cell loaded surgical mesh for tendon repair in rats.

Schon LC, Gill N, Thorpe M, Davis J, Nadaud J, Kim J, Molligan J, Zhang Z - J Transl Med (2014)

Design and application of MSC-loaded surgical mesh. A: Composition of MSC-loading mesh. B: Distribution of MSCs in the PGA-based scaffold. MSCs (green) were labeled with fluorescent dye SYTO®10. PGA fibers also showed green in auto-fluorescence (bar = 50 μm). C: The MSC-loaded mesh was used to repair the 3-mm defect (between the dot lines) created at the junction of Achilles tendon and gastrocnemius/soleus. The portion of scaffold/MSCs was inserted into the defect and surgical mesh was sutured to the surface of muscle and tendon (black arrowhead indicates a knot of surgical suture used to tie the scaffold and surgical mesh together. The suture was melted after autoclave).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4020316&req=5

Figure 1: Design and application of MSC-loaded surgical mesh. A: Composition of MSC-loading mesh. B: Distribution of MSCs in the PGA-based scaffold. MSCs (green) were labeled with fluorescent dye SYTO®10. PGA fibers also showed green in auto-fluorescence (bar = 50 μm). C: The MSC-loaded mesh was used to repair the 3-mm defect (between the dot lines) created at the junction of Achilles tendon and gastrocnemius/soleus. The portion of scaffold/MSCs was inserted into the defect and surgical mesh was sutured to the surface of muscle and tendon (black arrowhead indicates a knot of surgical suture used to tie the scaffold and surgical mesh together. The suture was melted after autoclave).
Mentions: The second component of the MSC-loaded mesh was a layer of non-woven scaffold, 4 x 4 x 2 mm3, made of polyglycolic acid (PGA, 60 mg/ml in density, Lot # 10-001253A-1015, Biomedical Structures). The scaffold was attached to the center of the surgical mesh with one knot using a 4–0 monofilament suture. The purpose of the PGA scaffold was to support and attach the hydrogel component to the mesh (Figure 1A).

Bottom Line: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel.A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats.Application of the MSC-loaded mesh, as a new device and MSC delivery vehicle, may benefit to early functional recovery of the ruptured tendon.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Orthopaedic Surgery, MedStar Union Memorial Hospital, 3333 North Calvert Street, Johnston Professional Building, Suite 400, Baltimore, MD 21218, USA. lewschon@gmail.com.

ABSTRACT

Objectives: The purpose of this study was to investigate the efficacy of a composite surgical mesh for delivery of mesenchymal stem cells (MSCs) in tendon repair.

Methods: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel. A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats. The tendon defects were repaired with either 1) MSC-loaded mesh; or 2) surgical mesh only; or 3) routine surgical suture. Repaired tendons were harvested at days 6 and 14 for histology, which was scored on the bases of collagen organization, vascularity and cellularity, and immunohistochemisty of types I and III collagen.

Results: In comparison with the other two repair types, at day 6, the MSC-loaded mesh significantly improved the quality of the repaired tendons with dense and parallel collagen bundles, reduced vascularity and increased type I collagen. At day 14, the MSC-loaded mesh repaired tendons had better collagen formation and organization.

Conclusion: The MSC-loaded mesh enhanced early tendon healing, particularly the quality of collagen bundles. Application of the MSC-loaded mesh, as a new device and MSC delivery vehicle, may benefit to early functional recovery of the ruptured tendon.

Show MeSH
Related in: MedlinePlus