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Glucagon and insulin have opposite effects on tissue chromium distribution in an obese mouse model.

Lin C, Chen PW, Chen WY, Sun CC, Mao FC - J Diabetes Investig (2013)

Bottom Line: Glucagon is known to regulate carbohydrate metabolism and hyperglucagonemia plays a role in the development of hyperglycemia in diabetic subjects.In contrast, insulin challenge significantly decreased Cr levels in bone but increased them in the fat, liver and muscle.The results show that glucagon and insulin have opposite effects on Cr levels in bone, fat, liver, and muscle.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine National Chung-Hsing University Taichung Taiwan.

ABSTRACT

Aims/introduction: Previous studies have suggested that chromium (Cr) is an essential cofactor for normal carbohydrate metabolism and affects insulin sensitivity, especially in rodent models. Several factors, such as insulin challenge, high carbohydrate intake, and response to stress (e.g., in obesity), alter Cr excretion or distribution. Glucagon is known to regulate carbohydrate metabolism and hyperglucagonemia plays a role in the development of hyperglycemia in diabetic subjects.

Materials and methods: In the present study we investigated possible modulation of Cr levels by glucagon using an obese mouse model. Mice were kept on a high-fat diet and then used as an obesity model. These obese mice were injected with one dose of glucagon or insulin and Cr levels in their tissues were determined.

Results: In obese mice, glucagon challenge significantly increased Cr levels in bone but decreased them in the fat and liver. In contrast, insulin challenge significantly decreased Cr levels in bone but increased them in the fat, liver and muscle.

Conclusions: The results show that glucagon and insulin have opposite effects on Cr levels in bone, fat, liver, and muscle.

No MeSH data available.


Related in: MedlinePlus

Glucagon and insulin have opposite effects on tissue chromium (Cr) distribution in obese mice. Mice were fed high‐fat chow for 8 weeks and then injected intraperitoneally with saline (control; n = 7), glucagon (n = 7), or insulin (n = 7). Tissues samples were harvested and Cr levels measured 1 h after injection. Values are the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the saline control.
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jdi12097-fig-0002: Glucagon and insulin have opposite effects on tissue chromium (Cr) distribution in obese mice. Mice were fed high‐fat chow for 8 weeks and then injected intraperitoneally with saline (control; n = 7), glucagon (n = 7), or insulin (n = 7). Tissues samples were harvested and Cr levels measured 1 h after injection. Values are the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the saline control.

Mentions: To investigate the role of glucagon in modulation of tissue Cr levels in obese mice, the effects of glucagon challenge on tissue Cr distribution were analyzed. For comparison, one experimental group was challenged with insulin. As shown in Figure 2, glucagon challenge significantly increased Cr levels in bone, but decreased Cr levels in fat and liver. In contrast, insulin challenge significantly decreased Cr levels in bone, but increased them in fat, liver, and muscle. These findings show that glucagon and insulin have opposite effects on Cr levels in these selected tissues. Similar results have been found using other obese (B6.V‐Lepob/J) and diabetic (BKS.Cg‐+Leprdb/+Leprdb) mouse models (C‐C Sun, unpubl. obs., 2006).


Glucagon and insulin have opposite effects on tissue chromium distribution in an obese mouse model.

Lin C, Chen PW, Chen WY, Sun CC, Mao FC - J Diabetes Investig (2013)

Glucagon and insulin have opposite effects on tissue chromium (Cr) distribution in obese mice. Mice were fed high‐fat chow for 8 weeks and then injected intraperitoneally with saline (control; n = 7), glucagon (n = 7), or insulin (n = 7). Tissues samples were harvested and Cr levels measured 1 h after injection. Values are the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the saline control.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4020246&req=5

jdi12097-fig-0002: Glucagon and insulin have opposite effects on tissue chromium (Cr) distribution in obese mice. Mice were fed high‐fat chow for 8 weeks and then injected intraperitoneally with saline (control; n = 7), glucagon (n = 7), or insulin (n = 7). Tissues samples were harvested and Cr levels measured 1 h after injection. Values are the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the saline control.
Mentions: To investigate the role of glucagon in modulation of tissue Cr levels in obese mice, the effects of glucagon challenge on tissue Cr distribution were analyzed. For comparison, one experimental group was challenged with insulin. As shown in Figure 2, glucagon challenge significantly increased Cr levels in bone, but decreased Cr levels in fat and liver. In contrast, insulin challenge significantly decreased Cr levels in bone, but increased them in fat, liver, and muscle. These findings show that glucagon and insulin have opposite effects on Cr levels in these selected tissues. Similar results have been found using other obese (B6.V‐Lepob/J) and diabetic (BKS.Cg‐+Leprdb/+Leprdb) mouse models (C‐C Sun, unpubl. obs., 2006).

Bottom Line: Glucagon is known to regulate carbohydrate metabolism and hyperglucagonemia plays a role in the development of hyperglycemia in diabetic subjects.In contrast, insulin challenge significantly decreased Cr levels in bone but increased them in the fat, liver and muscle.The results show that glucagon and insulin have opposite effects on Cr levels in bone, fat, liver, and muscle.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine National Chung-Hsing University Taichung Taiwan.

ABSTRACT

Aims/introduction: Previous studies have suggested that chromium (Cr) is an essential cofactor for normal carbohydrate metabolism and affects insulin sensitivity, especially in rodent models. Several factors, such as insulin challenge, high carbohydrate intake, and response to stress (e.g., in obesity), alter Cr excretion or distribution. Glucagon is known to regulate carbohydrate metabolism and hyperglucagonemia plays a role in the development of hyperglycemia in diabetic subjects.

Materials and methods: In the present study we investigated possible modulation of Cr levels by glucagon using an obese mouse model. Mice were kept on a high-fat diet and then used as an obesity model. These obese mice were injected with one dose of glucagon or insulin and Cr levels in their tissues were determined.

Results: In obese mice, glucagon challenge significantly increased Cr levels in bone but decreased them in the fat and liver. In contrast, insulin challenge significantly decreased Cr levels in bone but increased them in the fat, liver and muscle.

Conclusions: The results show that glucagon and insulin have opposite effects on Cr levels in bone, fat, liver, and muscle.

No MeSH data available.


Related in: MedlinePlus