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Study on association between H-ras gene polymorphism and gastric adenocarcinoma risk.

Rostami M, Kalaei Z, Pourhoseingholi MA, Kadivar M - Gastroenterol Hepatol Bed Bench (2013)

Bottom Line: We observed a statistically significant between smoking and T81C polymorphism C-carrier genotypes (OR=3.98, 95%CI=1.831-8.68, P < 0.001) as this individual had three-time risk for GA.We did not show a significant association between three main genotypes and H. pylori infection for risk of GA.These results suggested that there is no relationship between T81C-HRAS polymorphism and gastric cancer risk in Iranian patients.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry department, Pasteur Institute of Iran, Tehran, Iran.

ABSTRACT

Aim: The aim of this study was to investigate relation between H-ras T81C polymorphism and some of the important risk factors in gastric adenocarcinoma (GA).

Background: GA is one of the leading causes of cancer death in most countries. RAS gene is an important member in the PI3K-AKT signaling and the single nucleotide polymorphism at H-rasc DNA position 81 has been demonstrated has an important role in tumor genesis.

Patients and methods: In this study, we carried out single-nucleotide polymorphism analysis in an Iranian population. A total of 100 patients with gastric adenocarcinoma and 100 controls were examined for the presence of T81C H-ras polymorphism using PCR- RFLP assay.

Results: Statistical analysis revealed no relationship significant between TT, TC, CC and risk of GA, but, there was a poorly relation between male patient with C-carrier genotype and increasing risk of GA (P=0.07). Also, we investigate effect of four important risk factors for GA. There was a statistically significant difference between increasing of age and susceptibility for GA (OR=1.106, 95%CI=1.073-1.139, P < 0.001). We observed a statistically significant between smoking and T81C polymorphism C-carrier genotypes (OR=3.98, 95%CI=1.831-8.68, P < 0.001) as this individual had three-time risk for GA. We did not show a significant association between three main genotypes and H. pylori infection for risk of GA.

Conclusion: These results suggested that there is no relationship between T81C-HRAS polymorphism and gastric cancer risk in Iranian patients. But, gender (male in our study) and the other risk factor described above have an important role in developing of GA.

No MeSH data available.


Related in: MedlinePlus

PCR-RFLP analysis of –T81C H-ras polymorphism; Lane 1: 50 bp DNA ladder; Lane2: PCR product (undigested); Lane 3: Homozygous -81TT genotype; Lane 4: Homozygous -81CC genotype; Lane5: Heterozygous -81TC genotype.
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Figure 0001: PCR-RFLP analysis of –T81C H-ras polymorphism; Lane 1: 50 bp DNA ladder; Lane2: PCR product (undigested); Lane 3: Homozygous -81TT genotype; Lane 4: Homozygous -81CC genotype; Lane5: Heterozygous -81TC genotype.

Mentions: Based on information from publicly available databases, such as dbSNP (http://www.ncbi.nlm.nih.gov/SNP/) we collected data on H-ras polymorphisms. Consequently, the exon 1 of H-ras was amplified by polymerase chain reaction as previous described (17). The sequence of forward and reverse primers which were used in order to amplification of a 200 bp DNA segment were 5’-CTTGGCAGGTGGGGCAGGAGA-3’ and 5’-GGCACCTGGACGGCGGCGCTAG-3'respectively. Briefly, PCR conditions were as follows: an initial denaturation step at 94° C for 5 min, followed by 35 cycles of denaturing at 94° C for 1 min, annealing at 59° C for1 min, and extension at 72° C for 1 min. The final extension step was continued at 72° C for 10 min. After PCR amplification, reaction products were digested using RFLP technique, with Draш restriction endonuclease (Fermentas, Lithuania) for 6 h at 37° C. Results were observed in 3% agarose gel stained with ethidium bromide (Figure 1). Three fragments were observed including: 145, 55 and 200 bp. In some samples, experiments were performed two times to confirm our experiment.


Study on association between H-ras gene polymorphism and gastric adenocarcinoma risk.

Rostami M, Kalaei Z, Pourhoseingholi MA, Kadivar M - Gastroenterol Hepatol Bed Bench (2013)

PCR-RFLP analysis of –T81C H-ras polymorphism; Lane 1: 50 bp DNA ladder; Lane2: PCR product (undigested); Lane 3: Homozygous -81TT genotype; Lane 4: Homozygous -81CC genotype; Lane5: Heterozygous -81TC genotype.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4017517&req=5

Figure 0001: PCR-RFLP analysis of –T81C H-ras polymorphism; Lane 1: 50 bp DNA ladder; Lane2: PCR product (undigested); Lane 3: Homozygous -81TT genotype; Lane 4: Homozygous -81CC genotype; Lane5: Heterozygous -81TC genotype.
Mentions: Based on information from publicly available databases, such as dbSNP (http://www.ncbi.nlm.nih.gov/SNP/) we collected data on H-ras polymorphisms. Consequently, the exon 1 of H-ras was amplified by polymerase chain reaction as previous described (17). The sequence of forward and reverse primers which were used in order to amplification of a 200 bp DNA segment were 5’-CTTGGCAGGTGGGGCAGGAGA-3’ and 5’-GGCACCTGGACGGCGGCGCTAG-3'respectively. Briefly, PCR conditions were as follows: an initial denaturation step at 94° C for 5 min, followed by 35 cycles of denaturing at 94° C for 1 min, annealing at 59° C for1 min, and extension at 72° C for 1 min. The final extension step was continued at 72° C for 10 min. After PCR amplification, reaction products were digested using RFLP technique, with Draш restriction endonuclease (Fermentas, Lithuania) for 6 h at 37° C. Results were observed in 3% agarose gel stained with ethidium bromide (Figure 1). Three fragments were observed including: 145, 55 and 200 bp. In some samples, experiments were performed two times to confirm our experiment.

Bottom Line: We observed a statistically significant between smoking and T81C polymorphism C-carrier genotypes (OR=3.98, 95%CI=1.831-8.68, P < 0.001) as this individual had three-time risk for GA.We did not show a significant association between three main genotypes and H. pylori infection for risk of GA.These results suggested that there is no relationship between T81C-HRAS polymorphism and gastric cancer risk in Iranian patients.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry department, Pasteur Institute of Iran, Tehran, Iran.

ABSTRACT

Aim: The aim of this study was to investigate relation between H-ras T81C polymorphism and some of the important risk factors in gastric adenocarcinoma (GA).

Background: GA is one of the leading causes of cancer death in most countries. RAS gene is an important member in the PI3K-AKT signaling and the single nucleotide polymorphism at H-rasc DNA position 81 has been demonstrated has an important role in tumor genesis.

Patients and methods: In this study, we carried out single-nucleotide polymorphism analysis in an Iranian population. A total of 100 patients with gastric adenocarcinoma and 100 controls were examined for the presence of T81C H-ras polymorphism using PCR- RFLP assay.

Results: Statistical analysis revealed no relationship significant between TT, TC, CC and risk of GA, but, there was a poorly relation between male patient with C-carrier genotype and increasing risk of GA (P=0.07). Also, we investigate effect of four important risk factors for GA. There was a statistically significant difference between increasing of age and susceptibility for GA (OR=1.106, 95%CI=1.073-1.139, P < 0.001). We observed a statistically significant between smoking and T81C polymorphism C-carrier genotypes (OR=3.98, 95%CI=1.831-8.68, P < 0.001) as this individual had three-time risk for GA. We did not show a significant association between three main genotypes and H. pylori infection for risk of GA.

Conclusion: These results suggested that there is no relationship between T81C-HRAS polymorphism and gastric cancer risk in Iranian patients. But, gender (male in our study) and the other risk factor described above have an important role in developing of GA.

No MeSH data available.


Related in: MedlinePlus