Limits...
Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran.

Nikbin VS, Alikhani MY, Shojaei H, Mousavi SF, Salmanzadeh-Ahrabi S, Aslani MM - Gastroenterol Hepatol Bed Bench (2012)

Bottom Line: Ribotyping yielded 9 different clusters.According to our results there was not a significant correlation between the results of serotyping and those of ribotyping.However, different serotypes of E. coli may belong to the same ribotype clusters and vice versa.

View Article: PubMed Central - PubMed

Affiliation: Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.

ABSTRACT

Aim: We intended to find out the diversity of EPEC isolates among asymptomatic or diarrheal children in Iran using ribotyping.

Background: Enteropathogenic Escherichia coli (EPEC) is responsible for gastroenteritis especially in young children.

Patients and methods: A total of 39 EPEC collected strains were serotyped and the presence of virulence genes as well as EAF plasmid among the strains was studied. Adherence assay was also performed. Clonal diversity of the isolates was investigated using ribotyping.

Results: Of 39 studied strains of E. coli, 6 serogroups of EPEC were represented. The presence of the stx gene was ascertained in 7 isolates and the eaeA, eaeB and bfpA genes were harbored by 5, 3 and 1 strains, respectively. Ribotyping yielded 9 different clusters.

Conclusion: According to our results there was not a significant correlation between the results of serotyping and those of ribotyping. However, different serotypes of E. coli may belong to the same ribotype clusters and vice versa.

No MeSH data available.


Related in: MedlinePlus

Dendrogram of 39 E. coli strains isolated from diarreal and asymptotic patients in this study.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4017452&req=5

Figure 0001: Dendrogram of 39 E. coli strains isolated from diarreal and asymptotic patients in this study.

Mentions: The ribotyping of the isolates yielded 9 different clusters with a ribotype pattern consisting of an average 10 fragments per lane (Figure 1). They included 4 ribotypes that contained a single strain. Ribotype I was the dominant comprising 14 strains which belonged to 5 serogroups. Seven stx positive non EPEC strains of E. coli did not produce the same ribotype pattern. In our study it was found that different EPEC serotypes disseminated randomly in different ribotype clusters. There was no association between ribotype patterns and clinical outcome of persons (Table 1).


Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran.

Nikbin VS, Alikhani MY, Shojaei H, Mousavi SF, Salmanzadeh-Ahrabi S, Aslani MM - Gastroenterol Hepatol Bed Bench (2012)

Dendrogram of 39 E. coli strains isolated from diarreal and asymptotic patients in this study.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4017452&req=5

Figure 0001: Dendrogram of 39 E. coli strains isolated from diarreal and asymptotic patients in this study.
Mentions: The ribotyping of the isolates yielded 9 different clusters with a ribotype pattern consisting of an average 10 fragments per lane (Figure 1). They included 4 ribotypes that contained a single strain. Ribotype I was the dominant comprising 14 strains which belonged to 5 serogroups. Seven stx positive non EPEC strains of E. coli did not produce the same ribotype pattern. In our study it was found that different EPEC serotypes disseminated randomly in different ribotype clusters. There was no association between ribotype patterns and clinical outcome of persons (Table 1).

Bottom Line: Ribotyping yielded 9 different clusters.According to our results there was not a significant correlation between the results of serotyping and those of ribotyping.However, different serotypes of E. coli may belong to the same ribotype clusters and vice versa.

View Article: PubMed Central - PubMed

Affiliation: Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.

ABSTRACT

Aim: We intended to find out the diversity of EPEC isolates among asymptomatic or diarrheal children in Iran using ribotyping.

Background: Enteropathogenic Escherichia coli (EPEC) is responsible for gastroenteritis especially in young children.

Patients and methods: A total of 39 EPEC collected strains were serotyped and the presence of virulence genes as well as EAF plasmid among the strains was studied. Adherence assay was also performed. Clonal diversity of the isolates was investigated using ribotyping.

Results: Of 39 studied strains of E. coli, 6 serogroups of EPEC were represented. The presence of the stx gene was ascertained in 7 isolates and the eaeA, eaeB and bfpA genes were harbored by 5, 3 and 1 strains, respectively. Ribotyping yielded 9 different clusters.

Conclusion: According to our results there was not a significant correlation between the results of serotyping and those of ribotyping. However, different serotypes of E. coli may belong to the same ribotype clusters and vice versa.

No MeSH data available.


Related in: MedlinePlus