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Proteomics analysis of MKN45 cell line before and after treatment with Lavender aqueous extract.

Zamanian-Azodi M, Rezaie-Tavirani M, Heydari-Kashal S, Kalantari S, Dailian S, Zali H - Gastroenterol Hepatol Bed Bench (2012)

Bottom Line: An aqueous extract of Lavender has shown therapeutic effects on the nervous system in the high doses based on in-vivo studies.Flow cytometry and proteomics techniques were applied to determining pharmaceutical mechanism of lavender cytotoxic effects.Flow cytometry findings indicate that Lavender extract had a cytotoxic effect upon the cell line.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Aim: In this study the anticancer activity of Lavender aqueous extract against MKN45 cell line was evaluated.

Background: Plant-based drugs are regarded as promising therapies. Lavender is a plant that has been cultivated from ancient times. An aqueous extract of Lavender has shown therapeutic effects on the nervous system in the high doses based on in-vivo studies. Gastric cancer is one of the frequent cancers in Iranian population. We therefore assessed the effect of Lavender upon a gastric cancer cell line.

Patients and methods: The MKN45 cancer cell line was selected for treatment with aqueous extract of Lavender. Survival of MKN45 cell line was studied in the presence of various concentrations of Lavender extract by MTT assay method. Morphological studies were performed via microscopic analyses. Flow cytometry and proteomics techniques were applied to determining pharmaceutical mechanism of lavender cytotoxic effects.

Results: The survival and morphological studies revealed anticancer characteristics of extract. Flow cytometry findings indicate that Lavender extract had a cytotoxic effect upon the cell line. Proteomics analysis identified a significant alternation in gastric cellular proteome expression after treating with the extract. Among 1000 spots, more than 700 spots showed changes in protein expression levels by informatics analysis. Of these proteins, expression of three cancer biomarkers, Annexin1, Anolase1 and HSP70 were suppressed by extract.

Conclusion: This study suggests that Lavender extract is cytotoxic and alter protein expression in a gastric cancer cell line.

No MeSH data available.


Related in: MedlinePlus

Comparison of 2DE gels presented as a control MKN45 (a) and treated cells with 30 g/mL concentration of Lavender extract (b). The gel of untreated cells is selected as reference gel and the spots are marked on this gel (c). Due to expanding dimension of alternations, protein clustering has been done (the result are not shown).
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Figure 0004: Comparison of 2DE gels presented as a control MKN45 (a) and treated cells with 30 g/mL concentration of Lavender extract (b). The gel of untreated cells is selected as reference gel and the spots are marked on this gel (c). Due to expanding dimension of alternations, protein clustering has been done (the result are not shown).

Mentions: As flow cytometry has revealed that necrosis is the main mechanism of the cell death, Proteomics was used for determining effect of the Lavender extract upon gene expression. It was expected that, in correspond to necrosis, rate of alternation in gene expression will be noticeable. A large number of proteins are candidates as biomarkers of malignant transformation expression. Proteomes of the gastric cancer cell line in the presence and absence of extract were provided and compared (See Figure 4). Surprisingly, there was a considerable difference in the proteomes of the cell line treated with Lavender extract and the control cell line. As shown in Figure 4, there are about 1000 spots that are comparable between two gels. Clustering as a logical method for proteins classification and also showing schematically representation of their relation is a useful method for better understanding of protein expression alteration (27).


Proteomics analysis of MKN45 cell line before and after treatment with Lavender aqueous extract.

Zamanian-Azodi M, Rezaie-Tavirani M, Heydari-Kashal S, Kalantari S, Dailian S, Zali H - Gastroenterol Hepatol Bed Bench (2012)

Comparison of 2DE gels presented as a control MKN45 (a) and treated cells with 30 g/mL concentration of Lavender extract (b). The gel of untreated cells is selected as reference gel and the spots are marked on this gel (c). Due to expanding dimension of alternations, protein clustering has been done (the result are not shown).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4017446&req=5

Figure 0004: Comparison of 2DE gels presented as a control MKN45 (a) and treated cells with 30 g/mL concentration of Lavender extract (b). The gel of untreated cells is selected as reference gel and the spots are marked on this gel (c). Due to expanding dimension of alternations, protein clustering has been done (the result are not shown).
Mentions: As flow cytometry has revealed that necrosis is the main mechanism of the cell death, Proteomics was used for determining effect of the Lavender extract upon gene expression. It was expected that, in correspond to necrosis, rate of alternation in gene expression will be noticeable. A large number of proteins are candidates as biomarkers of malignant transformation expression. Proteomes of the gastric cancer cell line in the presence and absence of extract were provided and compared (See Figure 4). Surprisingly, there was a considerable difference in the proteomes of the cell line treated with Lavender extract and the control cell line. As shown in Figure 4, there are about 1000 spots that are comparable between two gels. Clustering as a logical method for proteins classification and also showing schematically representation of their relation is a useful method for better understanding of protein expression alteration (27).

Bottom Line: An aqueous extract of Lavender has shown therapeutic effects on the nervous system in the high doses based on in-vivo studies.Flow cytometry and proteomics techniques were applied to determining pharmaceutical mechanism of lavender cytotoxic effects.Flow cytometry findings indicate that Lavender extract had a cytotoxic effect upon the cell line.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Aim: In this study the anticancer activity of Lavender aqueous extract against MKN45 cell line was evaluated.

Background: Plant-based drugs are regarded as promising therapies. Lavender is a plant that has been cultivated from ancient times. An aqueous extract of Lavender has shown therapeutic effects on the nervous system in the high doses based on in-vivo studies. Gastric cancer is one of the frequent cancers in Iranian population. We therefore assessed the effect of Lavender upon a gastric cancer cell line.

Patients and methods: The MKN45 cancer cell line was selected for treatment with aqueous extract of Lavender. Survival of MKN45 cell line was studied in the presence of various concentrations of Lavender extract by MTT assay method. Morphological studies were performed via microscopic analyses. Flow cytometry and proteomics techniques were applied to determining pharmaceutical mechanism of lavender cytotoxic effects.

Results: The survival and morphological studies revealed anticancer characteristics of extract. Flow cytometry findings indicate that Lavender extract had a cytotoxic effect upon the cell line. Proteomics analysis identified a significant alternation in gastric cellular proteome expression after treating with the extract. Among 1000 spots, more than 700 spots showed changes in protein expression levels by informatics analysis. Of these proteins, expression of three cancer biomarkers, Annexin1, Anolase1 and HSP70 were suppressed by extract.

Conclusion: This study suggests that Lavender extract is cytotoxic and alter protein expression in a gastric cancer cell line.

No MeSH data available.


Related in: MedlinePlus