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Proteomics analysis of MKN45 cell line before and after treatment with Lavender aqueous extract.

Zamanian-Azodi M, Rezaie-Tavirani M, Heydari-Kashal S, Kalantari S, Dailian S, Zali H - Gastroenterol Hepatol Bed Bench (2012)

Bottom Line: An aqueous extract of Lavender has shown therapeutic effects on the nervous system in the high doses based on in-vivo studies.Flow cytometry and proteomics techniques were applied to determining pharmaceutical mechanism of lavender cytotoxic effects.Flow cytometry findings indicate that Lavender extract had a cytotoxic effect upon the cell line.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Aim: In this study the anticancer activity of Lavender aqueous extract against MKN45 cell line was evaluated.

Background: Plant-based drugs are regarded as promising therapies. Lavender is a plant that has been cultivated from ancient times. An aqueous extract of Lavender has shown therapeutic effects on the nervous system in the high doses based on in-vivo studies. Gastric cancer is one of the frequent cancers in Iranian population. We therefore assessed the effect of Lavender upon a gastric cancer cell line.

Patients and methods: The MKN45 cancer cell line was selected for treatment with aqueous extract of Lavender. Survival of MKN45 cell line was studied in the presence of various concentrations of Lavender extract by MTT assay method. Morphological studies were performed via microscopic analyses. Flow cytometry and proteomics techniques were applied to determining pharmaceutical mechanism of lavender cytotoxic effects.

Results: The survival and morphological studies revealed anticancer characteristics of extract. Flow cytometry findings indicate that Lavender extract had a cytotoxic effect upon the cell line. Proteomics analysis identified a significant alternation in gastric cellular proteome expression after treating with the extract. Among 1000 spots, more than 700 spots showed changes in protein expression levels by informatics analysis. Of these proteins, expression of three cancer biomarkers, Annexin1, Anolase1 and HSP70 were suppressed by extract.

Conclusion: This study suggests that Lavender extract is cytotoxic and alter protein expression in a gastric cancer cell line.

No MeSH data available.


Related in: MedlinePlus

MKN45 cell morphology in the a) absence, b) presence of 150 g/mL, and c) presence of 30 g/mL concentration of extract
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Figure 0002: MKN45 cell morphology in the a) absence, b) presence of 150 g/mL, and c) presence of 30 g/mL concentration of extract

Mentions: Figures 1 represents viability of MKN45 cell line in the presence of filtered extract of Lavender. The extract has inhibited cell proliferation. MTT assay revealed that extract has a significant effect on cell survival. For more investigating the cell morphology in the presence and absence of extract is studied via microscopic assessments (Figure 2). The morphological study indicated that extract induced cell death accompanied by gross morphological change alteration. To identify the mechanisms of extract effect against the cell line, flow cytometry was applied; the results are presented in Figure 3. Flow cytometric findings indicated that the considerable numbers of cells (about %60) were dead via necrosis (see Figure 3, upper left) and that the rate of apoptosis was negligible (Figure 3).


Proteomics analysis of MKN45 cell line before and after treatment with Lavender aqueous extract.

Zamanian-Azodi M, Rezaie-Tavirani M, Heydari-Kashal S, Kalantari S, Dailian S, Zali H - Gastroenterol Hepatol Bed Bench (2012)

MKN45 cell morphology in the a) absence, b) presence of 150 g/mL, and c) presence of 30 g/mL concentration of extract
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4017446&req=5

Figure 0002: MKN45 cell morphology in the a) absence, b) presence of 150 g/mL, and c) presence of 30 g/mL concentration of extract
Mentions: Figures 1 represents viability of MKN45 cell line in the presence of filtered extract of Lavender. The extract has inhibited cell proliferation. MTT assay revealed that extract has a significant effect on cell survival. For more investigating the cell morphology in the presence and absence of extract is studied via microscopic assessments (Figure 2). The morphological study indicated that extract induced cell death accompanied by gross morphological change alteration. To identify the mechanisms of extract effect against the cell line, flow cytometry was applied; the results are presented in Figure 3. Flow cytometric findings indicated that the considerable numbers of cells (about %60) were dead via necrosis (see Figure 3, upper left) and that the rate of apoptosis was negligible (Figure 3).

Bottom Line: An aqueous extract of Lavender has shown therapeutic effects on the nervous system in the high doses based on in-vivo studies.Flow cytometry and proteomics techniques were applied to determining pharmaceutical mechanism of lavender cytotoxic effects.Flow cytometry findings indicate that Lavender extract had a cytotoxic effect upon the cell line.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Aim: In this study the anticancer activity of Lavender aqueous extract against MKN45 cell line was evaluated.

Background: Plant-based drugs are regarded as promising therapies. Lavender is a plant that has been cultivated from ancient times. An aqueous extract of Lavender has shown therapeutic effects on the nervous system in the high doses based on in-vivo studies. Gastric cancer is one of the frequent cancers in Iranian population. We therefore assessed the effect of Lavender upon a gastric cancer cell line.

Patients and methods: The MKN45 cancer cell line was selected for treatment with aqueous extract of Lavender. Survival of MKN45 cell line was studied in the presence of various concentrations of Lavender extract by MTT assay method. Morphological studies were performed via microscopic analyses. Flow cytometry and proteomics techniques were applied to determining pharmaceutical mechanism of lavender cytotoxic effects.

Results: The survival and morphological studies revealed anticancer characteristics of extract. Flow cytometry findings indicate that Lavender extract had a cytotoxic effect upon the cell line. Proteomics analysis identified a significant alternation in gastric cellular proteome expression after treating with the extract. Among 1000 spots, more than 700 spots showed changes in protein expression levels by informatics analysis. Of these proteins, expression of three cancer biomarkers, Annexin1, Anolase1 and HSP70 were suppressed by extract.

Conclusion: This study suggests that Lavender extract is cytotoxic and alter protein expression in a gastric cancer cell line.

No MeSH data available.


Related in: MedlinePlus