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Association of leptin receptor gene Gln223Arg polymorphism with susceptibility to colorectal cancer.

Karimi K, Arkani M, Safaei A, Pourhoseingholi MA, Mohebbi SR, Fatemi SR, Vafaei M - Gastroenterol Hepatol Bed Bench (2011)

Bottom Line: There was no statistically evidence of significant difference in genotype and allele frequencies between the cases with CRC and controls for the Gln223Arg polymorphism of LEPR, before or after adjusting for confounders (age, BMI, sex, and smoking status).Furthermore, no significant difference was observed between the CRC cases and controls by BMI, sex and smoking status.Our findings suggest that the LEPR Gln223Arg polymorphism is not associated with the risk of CRC in Iranian population.

View Article: PubMed Central - PubMed

Affiliation: Research Institute for Gastroenterology and Liver Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Aim: Leptin is a 16 kDa polypeptide hormone which secreted by adipose tissue and has an important role in energy balance, insulin pathway and inflammation, because of that it may play an important role in colorectal cancer (CRC). Leptin exerts its effect through the leptin receptor (LEPR) a member of the class I cytokine receptor family.

Background: We have investigated whether glutamine to arginine substitution (Gln223Arg) in exon 6 of the leptin receptor gene, has implications for susceptibility to CRC.

Patients and methods: Polymerase chain reaction (PCR) and restriction enzyme digestion (RFLP) was performed to evaluate the association between the Gln223Arg polymorphism of the LEPR and CRC risk in a case-control study in 346 subjects involving 173 cases with CRC and 173 controls.

Results: There was no statistically evidence of significant difference in genotype and allele frequencies between the cases with CRC and controls for the Gln223Arg polymorphism of LEPR, before or after adjusting for confounders (age, BMI, sex, and smoking status). Furthermore, no significant difference was observed between the CRC cases and controls by BMI, sex and smoking status.

Conclusion: Our findings suggest that the LEPR Gln223Arg polymorphism is not associated with the risk of CRC in Iranian population.

No MeSH data available.


Related in: MedlinePlus

Sequencing results for each genotype variation. (A) Arg, (B) Gln, (C) Gln/Arg.
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Figure 0003: Sequencing results for each genotype variation. (A) Arg, (B) Gln, (C) Gln/Arg.

Mentions: Genomic DNA was obtained from 5ml EDTA-anticoagulated whole blood by using the standard “phenol chloroform” protocol. The LEPR Gln223Arg polymorphism was examined by means of PCR and amplified 80 bp fragment that used forward (5’-AAACTCAACGACACTCTCCTT-3’) and reverse (5’ –TGAACTGACATTAGAGGTGAC-3’) primers for LEPR gene (21). The PCR reaction was started with an initial denaturation at 93°C for 10 min followed by 35 cycles of 93°C for 45 s, 57°C for 30 s, 72°C for 45 s, and final extension at 72°C for 10 min. To determine genotype differentiation within LEPR genes, restriction enzyme MspI was used to digest PCR products at 37°C for 3 hours. Digestion showed genotypes denoted: Gln/Gln (80bp), Gln/Arg (80,59 and 21bp), and Arg/Arg (59 and 21bp). Digested products were electrophoreses on 3% agarose gel. The concordance of genotyping was confirmed by duplicate analysis 10% of samples and DNA sequencing 5% of samples (Figures 2, 3) that all of them were selected randomly (all results were accurate).


Association of leptin receptor gene Gln223Arg polymorphism with susceptibility to colorectal cancer.

Karimi K, Arkani M, Safaei A, Pourhoseingholi MA, Mohebbi SR, Fatemi SR, Vafaei M - Gastroenterol Hepatol Bed Bench (2011)

Sequencing results for each genotype variation. (A) Arg, (B) Gln, (C) Gln/Arg.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4017430&req=5

Figure 0003: Sequencing results for each genotype variation. (A) Arg, (B) Gln, (C) Gln/Arg.
Mentions: Genomic DNA was obtained from 5ml EDTA-anticoagulated whole blood by using the standard “phenol chloroform” protocol. The LEPR Gln223Arg polymorphism was examined by means of PCR and amplified 80 bp fragment that used forward (5’-AAACTCAACGACACTCTCCTT-3’) and reverse (5’ –TGAACTGACATTAGAGGTGAC-3’) primers for LEPR gene (21). The PCR reaction was started with an initial denaturation at 93°C for 10 min followed by 35 cycles of 93°C for 45 s, 57°C for 30 s, 72°C for 45 s, and final extension at 72°C for 10 min. To determine genotype differentiation within LEPR genes, restriction enzyme MspI was used to digest PCR products at 37°C for 3 hours. Digestion showed genotypes denoted: Gln/Gln (80bp), Gln/Arg (80,59 and 21bp), and Arg/Arg (59 and 21bp). Digested products were electrophoreses on 3% agarose gel. The concordance of genotyping was confirmed by duplicate analysis 10% of samples and DNA sequencing 5% of samples (Figures 2, 3) that all of them were selected randomly (all results were accurate).

Bottom Line: There was no statistically evidence of significant difference in genotype and allele frequencies between the cases with CRC and controls for the Gln223Arg polymorphism of LEPR, before or after adjusting for confounders (age, BMI, sex, and smoking status).Furthermore, no significant difference was observed between the CRC cases and controls by BMI, sex and smoking status.Our findings suggest that the LEPR Gln223Arg polymorphism is not associated with the risk of CRC in Iranian population.

View Article: PubMed Central - PubMed

Affiliation: Research Institute for Gastroenterology and Liver Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Aim: Leptin is a 16 kDa polypeptide hormone which secreted by adipose tissue and has an important role in energy balance, insulin pathway and inflammation, because of that it may play an important role in colorectal cancer (CRC). Leptin exerts its effect through the leptin receptor (LEPR) a member of the class I cytokine receptor family.

Background: We have investigated whether glutamine to arginine substitution (Gln223Arg) in exon 6 of the leptin receptor gene, has implications for susceptibility to CRC.

Patients and methods: Polymerase chain reaction (PCR) and restriction enzyme digestion (RFLP) was performed to evaluate the association between the Gln223Arg polymorphism of the LEPR and CRC risk in a case-control study in 346 subjects involving 173 cases with CRC and 173 controls.

Results: There was no statistically evidence of significant difference in genotype and allele frequencies between the cases with CRC and controls for the Gln223Arg polymorphism of LEPR, before or after adjusting for confounders (age, BMI, sex, and smoking status). Furthermore, no significant difference was observed between the CRC cases and controls by BMI, sex and smoking status.

Conclusion: Our findings suggest that the LEPR Gln223Arg polymorphism is not associated with the risk of CRC in Iranian population.

No MeSH data available.


Related in: MedlinePlus