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A model describing the use of a bronchial blocking device and a sheathed bronchoscope for pulmonary aspiration studies in the Gottingen minipig.

Hulse E, Reed FC, Eddleston M, Etherington R, Clutton RE - Lab. Anim. (2014)

Bottom Line: The administration of test substances into a single lung, or lung lobe, allows the remaining untreated lung to act as an experimental control and effectively halves the number of animals required in a given experiment.However, the ease of substance administration and the subsequent analysis of its effects, for example by bronchoalveolar lavage or bronchoscopy, depend critically on the size of the animal model.The advantages of using minipigs; ease of handling, reduced housing requirements, genetic homogeneity, etc. are reduced if their diminutive size makes lung studies difficult.

View Article: PubMed Central - PubMed

Affiliation: Pharmacology, Toxicology and Therapeutics, Centre for Cardiovascular Science, Queens Medical Research Institute, University of Edinburgh, Edinburgh, UK.

ABSTRACT
The administration of test substances into a single lung, or lung lobe, allows the remaining untreated lung to act as an experimental control and effectively halves the number of animals required in a given experiment. It reduces the likelihood of early fatal pulmonary failure when noxious substances are studied which may lessen the need for replacement animals. However, the ease of substance administration and the subsequent analysis of its effects, for example by bronchoalveolar lavage or bronchoscopy, depend critically on the size of the animal model. The advantages of using minipigs; ease of handling, reduced housing requirements, genetic homogeneity, etc. are reduced if their diminutive size makes lung studies difficult. This article describes the use of a bronchial blocking device and a sheathed bronchoscope which enabled sterile endobronchial substance administration in Göttingen minipigs, and allowed pulmonary aspiration studies to be conducted with each animal acting as its own control.

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Diagram illustrating the instrumentation used in lung aspiration studies in Göttingen minipigs. The lungs and devices are to approximate scale. A: breathing hoses from Seimens Servo 300 A ventilator; B: bronchoscope dual-axis swivel adapter; C: bronchial blocker inflation line; D: endotracheal tube cuff inflater; E: torque controlled bronchial blocker (TCB; Univent endotracheal tube); F: right accessory bronchus; G: bronchial blocker cuff; H: bronchoscope (BRS-5000); edc: epidural catheter.
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fig1-0023677213518526: Diagram illustrating the instrumentation used in lung aspiration studies in Göttingen minipigs. The lungs and devices are to approximate scale. A: breathing hoses from Seimens Servo 300 A ventilator; B: bronchoscope dual-axis swivel adapter; C: bronchial blocker inflation line; D: endotracheal tube cuff inflater; E: torque controlled bronchial blocker (TCB; Univent endotracheal tube); F: right accessory bronchus; G: bronchial blocker cuff; H: bronchoscope (BRS-5000); edc: epidural catheter.

Mentions: In many pulmonary function studies, treatments are applied to both lungs of individual animals and the effects compared with function in untreated controls. The use of bronchial blockers in a selected bronchus ensures treatments are directed to the contralateral lung, permitting the ‘blocked’ lung to act as a control. This effectively halves the number of animals required per study. Bronchial blockers also allow the study of direct (or topical) versus indirect (systemic) effects of treatment on lung structure and function. Similar advantages also result when double lumen endotracheal tubes (DLTs) are used. However, using right-sided DLTs in pigs is likely to obstruct the right accessory bronchus (F; Figure 1) causing right apical lung collapse and increased intrapulmonary shunt. DLTs also require frequent bronchoscopic repositioning over a 48 h period and can themselves cause bronchial damage.Figure 1.


A model describing the use of a bronchial blocking device and a sheathed bronchoscope for pulmonary aspiration studies in the Gottingen minipig.

Hulse E, Reed FC, Eddleston M, Etherington R, Clutton RE - Lab. Anim. (2014)

Diagram illustrating the instrumentation used in lung aspiration studies in Göttingen minipigs. The lungs and devices are to approximate scale. A: breathing hoses from Seimens Servo 300 A ventilator; B: bronchoscope dual-axis swivel adapter; C: bronchial blocker inflation line; D: endotracheal tube cuff inflater; E: torque controlled bronchial blocker (TCB; Univent endotracheal tube); F: right accessory bronchus; G: bronchial blocker cuff; H: bronchoscope (BRS-5000); edc: epidural catheter.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4017320&req=5

fig1-0023677213518526: Diagram illustrating the instrumentation used in lung aspiration studies in Göttingen minipigs. The lungs and devices are to approximate scale. A: breathing hoses from Seimens Servo 300 A ventilator; B: bronchoscope dual-axis swivel adapter; C: bronchial blocker inflation line; D: endotracheal tube cuff inflater; E: torque controlled bronchial blocker (TCB; Univent endotracheal tube); F: right accessory bronchus; G: bronchial blocker cuff; H: bronchoscope (BRS-5000); edc: epidural catheter.
Mentions: In many pulmonary function studies, treatments are applied to both lungs of individual animals and the effects compared with function in untreated controls. The use of bronchial blockers in a selected bronchus ensures treatments are directed to the contralateral lung, permitting the ‘blocked’ lung to act as a control. This effectively halves the number of animals required per study. Bronchial blockers also allow the study of direct (or topical) versus indirect (systemic) effects of treatment on lung structure and function. Similar advantages also result when double lumen endotracheal tubes (DLTs) are used. However, using right-sided DLTs in pigs is likely to obstruct the right accessory bronchus (F; Figure 1) causing right apical lung collapse and increased intrapulmonary shunt. DLTs also require frequent bronchoscopic repositioning over a 48 h period and can themselves cause bronchial damage.Figure 1.

Bottom Line: The administration of test substances into a single lung, or lung lobe, allows the remaining untreated lung to act as an experimental control and effectively halves the number of animals required in a given experiment.However, the ease of substance administration and the subsequent analysis of its effects, for example by bronchoalveolar lavage or bronchoscopy, depend critically on the size of the animal model.The advantages of using minipigs; ease of handling, reduced housing requirements, genetic homogeneity, etc. are reduced if their diminutive size makes lung studies difficult.

View Article: PubMed Central - PubMed

Affiliation: Pharmacology, Toxicology and Therapeutics, Centre for Cardiovascular Science, Queens Medical Research Institute, University of Edinburgh, Edinburgh, UK.

ABSTRACT
The administration of test substances into a single lung, or lung lobe, allows the remaining untreated lung to act as an experimental control and effectively halves the number of animals required in a given experiment. It reduces the likelihood of early fatal pulmonary failure when noxious substances are studied which may lessen the need for replacement animals. However, the ease of substance administration and the subsequent analysis of its effects, for example by bronchoalveolar lavage or bronchoscopy, depend critically on the size of the animal model. The advantages of using minipigs; ease of handling, reduced housing requirements, genetic homogeneity, etc. are reduced if their diminutive size makes lung studies difficult. This article describes the use of a bronchial blocking device and a sheathed bronchoscope which enabled sterile endobronchial substance administration in Göttingen minipigs, and allowed pulmonary aspiration studies to be conducted with each animal acting as its own control.

Show MeSH
Related in: MedlinePlus