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Tal2 expression is induced by all-trans retinoic acid in P19 cells prior to acquisition of neural fate.

Kobayashi T, Komori R, Ishida K, Kino K, Tanuma S, Miyazawa H - Sci Rep (2014)

Bottom Line: We found that the expression of Tal2 gene was induced in P19 cells after addition of atRA in suspension culture.Moreover, we showed that atRA and retinoic acid receptor α regulated Tal2 expression.These results demonstrate for the first time that atRA induces Tal2 expression in P19 cells, and suggest that TAL2 commits to the acquisition of neural fate in brain development.

View Article: PubMed Central - PubMed

Affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193, Japan.

ABSTRACT
TAL2 is a member of the basic helix-loop-helix family and is essential for the normal development of the mouse brain. However, the function of TAL2 during brain development is unclear. P19 cells are pluripotent mouse embryonal carcinoma cells that adopt neural fates upon exposure to all-trans retinoic acid (atRA) and culture in suspension. We found that the expression of Tal2 gene was induced in P19 cells after addition of atRA in suspension culture. Tal2 expression was detected within 3 h after the induction, and had nearly returned to basal levels by 24 h. When GFP-tagged TAL2 (GFP-TAL2) was expressed in P19 cells, we observed GFP-TAL2 in the nucleus. Moreover, we showed that atRA and retinoic acid receptor α regulated Tal2 expression. These results demonstrate for the first time that atRA induces Tal2 expression in P19 cells, and suggest that TAL2 commits to the acquisition of neural fate in brain development.

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The localization of GFP-TAL2 visualized by fluorescence microscopy.P19 cells were transfected with the expression vector encoding GFP-TAL2 and were stained with Hoechst33258 48 h after transfection. (a) Phase-contrast. (b) GFP-TAL2. (c) Hoechst33258. (d) Overlay.
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f2: The localization of GFP-TAL2 visualized by fluorescence microscopy.P19 cells were transfected with the expression vector encoding GFP-TAL2 and were stained with Hoechst33258 48 h after transfection. (a) Phase-contrast. (b) GFP-TAL2. (c) Hoechst33258. (d) Overlay.

Mentions: To examine the behavior of TAL2 in P19 cells, we generated GFP-tagged TAL2 (GFP-TAL2) expression construct. This construct was introduced into P19 cells via transfection, and these cells were stained with Hoechst33258 48 h post transfection. We then used fluorescence microscopy to visualize these cells (Fig. 2). GFP-TAL2 expressed in P19 cells was detected in nuclei.


Tal2 expression is induced by all-trans retinoic acid in P19 cells prior to acquisition of neural fate.

Kobayashi T, Komori R, Ishida K, Kino K, Tanuma S, Miyazawa H - Sci Rep (2014)

The localization of GFP-TAL2 visualized by fluorescence microscopy.P19 cells were transfected with the expression vector encoding GFP-TAL2 and were stained with Hoechst33258 48 h after transfection. (a) Phase-contrast. (b) GFP-TAL2. (c) Hoechst33258. (d) Overlay.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4017210&req=5

f2: The localization of GFP-TAL2 visualized by fluorescence microscopy.P19 cells were transfected with the expression vector encoding GFP-TAL2 and were stained with Hoechst33258 48 h after transfection. (a) Phase-contrast. (b) GFP-TAL2. (c) Hoechst33258. (d) Overlay.
Mentions: To examine the behavior of TAL2 in P19 cells, we generated GFP-tagged TAL2 (GFP-TAL2) expression construct. This construct was introduced into P19 cells via transfection, and these cells were stained with Hoechst33258 48 h post transfection. We then used fluorescence microscopy to visualize these cells (Fig. 2). GFP-TAL2 expressed in P19 cells was detected in nuclei.

Bottom Line: We found that the expression of Tal2 gene was induced in P19 cells after addition of atRA in suspension culture.Moreover, we showed that atRA and retinoic acid receptor α regulated Tal2 expression.These results demonstrate for the first time that atRA induces Tal2 expression in P19 cells, and suggest that TAL2 commits to the acquisition of neural fate in brain development.

View Article: PubMed Central - PubMed

Affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193, Japan.

ABSTRACT
TAL2 is a member of the basic helix-loop-helix family and is essential for the normal development of the mouse brain. However, the function of TAL2 during brain development is unclear. P19 cells are pluripotent mouse embryonal carcinoma cells that adopt neural fates upon exposure to all-trans retinoic acid (atRA) and culture in suspension. We found that the expression of Tal2 gene was induced in P19 cells after addition of atRA in suspension culture. Tal2 expression was detected within 3 h after the induction, and had nearly returned to basal levels by 24 h. When GFP-tagged TAL2 (GFP-TAL2) was expressed in P19 cells, we observed GFP-TAL2 in the nucleus. Moreover, we showed that atRA and retinoic acid receptor α regulated Tal2 expression. These results demonstrate for the first time that atRA induces Tal2 expression in P19 cells, and suggest that TAL2 commits to the acquisition of neural fate in brain development.

Show MeSH
Related in: MedlinePlus