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Physical exercise reduces the expression of RANTES and its CCR5 receptor in the adipose tissue of obese humans.

Baturcam E, Abubaker J, Tiss A, Abu-Farha M, Khadir A, Al-Ghimlas F, Al-Khairi I, Cherian P, Elkum N, Hammad M, John J, Kavalakatt S, Lehe C, Warsame S, Behbehani K, Dermime S, Dehbi M - Mediators Inflamm. (2014)

Bottom Line: Fifty-seven adult nondiabetic subjects (17 lean and 40 obese) were enrolled in a 3-month supervised physical exercise.Physical exercise significantly reduced the expression of both RANTES and CCR5 (P < 0.05) in the adipose tissue of obese individuals with a concomitant decrease in the levels of the inflammatory markers TNF- α , IL-6, and P-JNK.Circulating RANTES correlated negatively with anti-inflammatory IL-1 ra (P = 0.001) and positively with proinflammatory IP-10 and TBARS levels (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait ; Queensland Children's Medical Research Institute, University of Queensland, Brisbane, QLD 4029, Australia.

ABSTRACT
RANTES and its CCR5 receptor trigger inflammation and its progression to insulin resistance in obese. In the present study, we investigated for the first time the effect of physical exercise on the expression of RANTES and CCR5 in obese humans. Fifty-seven adult nondiabetic subjects (17 lean and 40 obese) were enrolled in a 3-month supervised physical exercise. RANTES and CCR5 expressions were measured in PBMCs and subcutaneous adipose tissue before and after exercise. Circulating plasma levels of RANTES were also investigated. There was a significant increase in RANTES and CCR5 expression in the subcutaneous adipose tissue of obese compared to lean. In PBMCs, however, while the levels of RANTES mRNA and protein were comparable between both groups, CCR5 mRNA was downregulated in obese subjects (P < 0.05). Physical exercise significantly reduced the expression of both RANTES and CCR5 (P < 0.05) in the adipose tissue of obese individuals with a concomitant decrease in the levels of the inflammatory markers TNF- α , IL-6, and P-JNK. Circulating RANTES correlated negatively with anti-inflammatory IL-1 ra (P = 0.001) and positively with proinflammatory IP-10 and TBARS levels (P < 0.05). Therefore, physical exercise may provide an effective approach for combating the deleterious effects associated with obesity through RANTES signaling in the adipose tissue.

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Physical exercise reduces the expression of RANTES and CCR5 in the adipose tissue. (a) IHC staining with RANTES antibody using subcutaneous adipose tissues from obese subjects before and after 3 months of physical exercise. Aperio software was used to quantify positive staining in obese before and after physical exercise. The data are plotted in a bar graph on the right of the figure as fold changes of RANTES protein expression in obese subjects before and after the physical exercise program. (b) qRT-PCR analysis of RANTES, CCR5, TNF-α, and IL-6 mRNA expression in the adipose tissue from obese before and after 3 months of physical exercise. (c) Graphic representation of IHC staining with TNF-α, anti-IL-6, and anti-Phospho-JNK antibodies using subcutaneous adipose tissues from obese subjects before and after 3 months of physical exercise. Aperio software was used to quantify positive staining in obese before and after physical exercise. Paired t-test for two group analysis was done to compare the expression of proteins and mRNA in obese before and after exercise. For each experiment, the sample size from each group is indicated by n.
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fig4: Physical exercise reduces the expression of RANTES and CCR5 in the adipose tissue. (a) IHC staining with RANTES antibody using subcutaneous adipose tissues from obese subjects before and after 3 months of physical exercise. Aperio software was used to quantify positive staining in obese before and after physical exercise. The data are plotted in a bar graph on the right of the figure as fold changes of RANTES protein expression in obese subjects before and after the physical exercise program. (b) qRT-PCR analysis of RANTES, CCR5, TNF-α, and IL-6 mRNA expression in the adipose tissue from obese before and after 3 months of physical exercise. (c) Graphic representation of IHC staining with TNF-α, anti-IL-6, and anti-Phospho-JNK antibodies using subcutaneous adipose tissues from obese subjects before and after 3 months of physical exercise. Aperio software was used to quantify positive staining in obese before and after physical exercise. Paired t-test for two group analysis was done to compare the expression of proteins and mRNA in obese before and after exercise. For each experiment, the sample size from each group is indicated by n.

Mentions: We previously reported the effectiveness of our physical exercise protocol on improving the physical, clinical, and metabolic parameters on obese subjects [38]. Accordingly, there was a significant reduction of PBF and SBP and increase in VO2Max⁡ along with a decrease in TBARS levels and a reduction of inflammatory markers TNF-α and IL-6 in the circulation [38]. However, physical exercise did not reduce the levels of RANTES in the circulation [38]. To investigate whether physical exercise has an impact on the endogenous expression of RANTES and CCR5, qRT-PCR and IHC were carried out on adipose tissue from obese subjects before and after the exercise program. As shown in Figure 4(a), IHC carried out on adipose tissue from obese subjects before (n = 11) and after (n = 7) the exercise program indicated a significant decrease in the expression of RANTES by physical exercise (P = 0.003). qRT-PCR performed on obese before and after the exercise program (n = 10 for each group) confirmed the reduction of RANTES mRNA expression by physical exercise (P = 0.01, Figure 4(b)). Likewise, CCR5 mRNA was significantly reduced by physical exercise in the adipose tissue (P = 0.02, Figure 4(b)). Using the same samples, we observed a decrease in the endogenous expression of TNF-α and IL-6 both at the mRNA level (P < 0.05, Figure 4(b)) and protein level (P < 0.05, Figure 4(c)).


Physical exercise reduces the expression of RANTES and its CCR5 receptor in the adipose tissue of obese humans.

Baturcam E, Abubaker J, Tiss A, Abu-Farha M, Khadir A, Al-Ghimlas F, Al-Khairi I, Cherian P, Elkum N, Hammad M, John J, Kavalakatt S, Lehe C, Warsame S, Behbehani K, Dermime S, Dehbi M - Mediators Inflamm. (2014)

Physical exercise reduces the expression of RANTES and CCR5 in the adipose tissue. (a) IHC staining with RANTES antibody using subcutaneous adipose tissues from obese subjects before and after 3 months of physical exercise. Aperio software was used to quantify positive staining in obese before and after physical exercise. The data are plotted in a bar graph on the right of the figure as fold changes of RANTES protein expression in obese subjects before and after the physical exercise program. (b) qRT-PCR analysis of RANTES, CCR5, TNF-α, and IL-6 mRNA expression in the adipose tissue from obese before and after 3 months of physical exercise. (c) Graphic representation of IHC staining with TNF-α, anti-IL-6, and anti-Phospho-JNK antibodies using subcutaneous adipose tissues from obese subjects before and after 3 months of physical exercise. Aperio software was used to quantify positive staining in obese before and after physical exercise. Paired t-test for two group analysis was done to compare the expression of proteins and mRNA in obese before and after exercise. For each experiment, the sample size from each group is indicated by n.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig4: Physical exercise reduces the expression of RANTES and CCR5 in the adipose tissue. (a) IHC staining with RANTES antibody using subcutaneous adipose tissues from obese subjects before and after 3 months of physical exercise. Aperio software was used to quantify positive staining in obese before and after physical exercise. The data are plotted in a bar graph on the right of the figure as fold changes of RANTES protein expression in obese subjects before and after the physical exercise program. (b) qRT-PCR analysis of RANTES, CCR5, TNF-α, and IL-6 mRNA expression in the adipose tissue from obese before and after 3 months of physical exercise. (c) Graphic representation of IHC staining with TNF-α, anti-IL-6, and anti-Phospho-JNK antibodies using subcutaneous adipose tissues from obese subjects before and after 3 months of physical exercise. Aperio software was used to quantify positive staining in obese before and after physical exercise. Paired t-test for two group analysis was done to compare the expression of proteins and mRNA in obese before and after exercise. For each experiment, the sample size from each group is indicated by n.
Mentions: We previously reported the effectiveness of our physical exercise protocol on improving the physical, clinical, and metabolic parameters on obese subjects [38]. Accordingly, there was a significant reduction of PBF and SBP and increase in VO2Max⁡ along with a decrease in TBARS levels and a reduction of inflammatory markers TNF-α and IL-6 in the circulation [38]. However, physical exercise did not reduce the levels of RANTES in the circulation [38]. To investigate whether physical exercise has an impact on the endogenous expression of RANTES and CCR5, qRT-PCR and IHC were carried out on adipose tissue from obese subjects before and after the exercise program. As shown in Figure 4(a), IHC carried out on adipose tissue from obese subjects before (n = 11) and after (n = 7) the exercise program indicated a significant decrease in the expression of RANTES by physical exercise (P = 0.003). qRT-PCR performed on obese before and after the exercise program (n = 10 for each group) confirmed the reduction of RANTES mRNA expression by physical exercise (P = 0.01, Figure 4(b)). Likewise, CCR5 mRNA was significantly reduced by physical exercise in the adipose tissue (P = 0.02, Figure 4(b)). Using the same samples, we observed a decrease in the endogenous expression of TNF-α and IL-6 both at the mRNA level (P < 0.05, Figure 4(b)) and protein level (P < 0.05, Figure 4(c)).

Bottom Line: Fifty-seven adult nondiabetic subjects (17 lean and 40 obese) were enrolled in a 3-month supervised physical exercise.Physical exercise significantly reduced the expression of both RANTES and CCR5 (P < 0.05) in the adipose tissue of obese individuals with a concomitant decrease in the levels of the inflammatory markers TNF- α , IL-6, and P-JNK.Circulating RANTES correlated negatively with anti-inflammatory IL-1 ra (P = 0.001) and positively with proinflammatory IP-10 and TBARS levels (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait ; Queensland Children's Medical Research Institute, University of Queensland, Brisbane, QLD 4029, Australia.

ABSTRACT
RANTES and its CCR5 receptor trigger inflammation and its progression to insulin resistance in obese. In the present study, we investigated for the first time the effect of physical exercise on the expression of RANTES and CCR5 in obese humans. Fifty-seven adult nondiabetic subjects (17 lean and 40 obese) were enrolled in a 3-month supervised physical exercise. RANTES and CCR5 expressions were measured in PBMCs and subcutaneous adipose tissue before and after exercise. Circulating plasma levels of RANTES were also investigated. There was a significant increase in RANTES and CCR5 expression in the subcutaneous adipose tissue of obese compared to lean. In PBMCs, however, while the levels of RANTES mRNA and protein were comparable between both groups, CCR5 mRNA was downregulated in obese subjects (P < 0.05). Physical exercise significantly reduced the expression of both RANTES and CCR5 (P < 0.05) in the adipose tissue of obese individuals with a concomitant decrease in the levels of the inflammatory markers TNF- α , IL-6, and P-JNK. Circulating RANTES correlated negatively with anti-inflammatory IL-1 ra (P = 0.001) and positively with proinflammatory IP-10 and TBARS levels (P < 0.05). Therefore, physical exercise may provide an effective approach for combating the deleterious effects associated with obesity through RANTES signaling in the adipose tissue.

Show MeSH
Related in: MedlinePlus