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Libidibia ferrea mature seeds promote antinociceptive effect by peripheral and central pathway: possible involvement of opioid and cholinergic receptors.

Sawada LA, Monteiro VS, Rabelo GR, Dias GB, Da Cunha M, do Nascimento JL, Bastos Gde N - Biomed Res Int (2014)

Bottom Line: We evaluated the antinociceptive effect in the LF aqueous seed extract and Lipidic Portion of Libidibia ferrea (LPLF), partially elucidating their mechanisms.LF aqueous seed extract and LPLF demonstrated a dose-dependent antinociceptive effect in all tests indicating both peripheral anti-inflammatory and central analgesia properties.Together, the present results suggested that Libidibia ferrea induced antinociceptive activity is possibly related to its ability to inhibit opioid, cholinergic receptors, and cyclooxygenase-2 pathway, since its main component, linoleic acid, has been demonstrated to produce such effect in previous studies.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Neuroquímica Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal do Pará (UFPA), Rua Augusto Correa s/n, Guamá, 66075-900 Belém, PA, Brazil ; Laboratório de Neuroinflamação, Instituto de Ciências Biológicas, Universidade Federal do Pará (UFPA), Rua Augusto Correa s/n, Guamá, 66075-900 Belém, PA, Brazil.

ABSTRACT
Libidibia ferrea (LF) is a medicinal plant that holds many pharmacological properties. We evaluated the antinociceptive effect in the LF aqueous seed extract and Lipidic Portion of Libidibia ferrea (LPLF), partially elucidating their mechanisms. Histochemical tests and Gas chromatography of the LPLF were performed to characterize its fatty acids. Acetic acid-induced abdominal constriction, formalin-induced pain, and hot-plate test in mice were employed in the study. In all experiments, aqueous extract or LPLF was administered systemically at the doses of 1, 5, and 10 mg/kg. LF aqueous seed extract and LPLF demonstrated a dose-dependent antinociceptive effect in all tests indicating both peripheral anti-inflammatory and central analgesia properties. Also, the use of atropine (5 mg/kg), naloxone (5 mg/kg) in the abdominal writhing test was able to reverse the antinociceptive effect of the LPLF, indicating that at least one of LF lipids components is responsible for the dose related antinociceptive action in chemical and thermal models of nociception in mice. Together, the present results suggested that Libidibia ferrea induced antinociceptive activity is possibly related to its ability to inhibit opioid, cholinergic receptors, and cyclooxygenase-2 pathway, since its main component, linoleic acid, has been demonstrated to produce such effect in previous studies.

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Related in: MedlinePlus

The effects of different antagonists on the antinociceptive activity of the LPLF in the acetic acid-induced contortions. Animals were pretreated with naloxone (5 mg/kg, i.p.), atropine (5 mg/kg, i.p.), or celecoxib (5 mg/kg, i.p.) 30 min prior to oral administration of the Libidibia ferrea seed oil (10 mg/kg, p.o.). The results are presented as the mean ± S.E.M (n = 6) of total writhings. ***P < 0.01 compared to control group, Tukey's multiple comparison test.
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fig6: The effects of different antagonists on the antinociceptive activity of the LPLF in the acetic acid-induced contortions. Animals were pretreated with naloxone (5 mg/kg, i.p.), atropine (5 mg/kg, i.p.), or celecoxib (5 mg/kg, i.p.) 30 min prior to oral administration of the Libidibia ferrea seed oil (10 mg/kg, p.o.). The results are presented as the mean ± S.E.M (n = 6) of total writhings. ***P < 0.01 compared to control group, Tukey's multiple comparison test.

Mentions: Also, the inhibition of peripheral inflammatory pain was further investigated with the administration of celecoxib (2 h prior the experiment) and then with LPLF (1 h prior the experiment), which increased the analgesic effect of the LPLF, demonstrating that LPLF probably causes an anti-inflammatory effect in a COX-2 select pathway (Figure 6).


Libidibia ferrea mature seeds promote antinociceptive effect by peripheral and central pathway: possible involvement of opioid and cholinergic receptors.

Sawada LA, Monteiro VS, Rabelo GR, Dias GB, Da Cunha M, do Nascimento JL, Bastos Gde N - Biomed Res Int (2014)

The effects of different antagonists on the antinociceptive activity of the LPLF in the acetic acid-induced contortions. Animals were pretreated with naloxone (5 mg/kg, i.p.), atropine (5 mg/kg, i.p.), or celecoxib (5 mg/kg, i.p.) 30 min prior to oral administration of the Libidibia ferrea seed oil (10 mg/kg, p.o.). The results are presented as the mean ± S.E.M (n = 6) of total writhings. ***P < 0.01 compared to control group, Tukey's multiple comparison test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016940&req=5

fig6: The effects of different antagonists on the antinociceptive activity of the LPLF in the acetic acid-induced contortions. Animals were pretreated with naloxone (5 mg/kg, i.p.), atropine (5 mg/kg, i.p.), or celecoxib (5 mg/kg, i.p.) 30 min prior to oral administration of the Libidibia ferrea seed oil (10 mg/kg, p.o.). The results are presented as the mean ± S.E.M (n = 6) of total writhings. ***P < 0.01 compared to control group, Tukey's multiple comparison test.
Mentions: Also, the inhibition of peripheral inflammatory pain was further investigated with the administration of celecoxib (2 h prior the experiment) and then with LPLF (1 h prior the experiment), which increased the analgesic effect of the LPLF, demonstrating that LPLF probably causes an anti-inflammatory effect in a COX-2 select pathway (Figure 6).

Bottom Line: We evaluated the antinociceptive effect in the LF aqueous seed extract and Lipidic Portion of Libidibia ferrea (LPLF), partially elucidating their mechanisms.LF aqueous seed extract and LPLF demonstrated a dose-dependent antinociceptive effect in all tests indicating both peripheral anti-inflammatory and central analgesia properties.Together, the present results suggested that Libidibia ferrea induced antinociceptive activity is possibly related to its ability to inhibit opioid, cholinergic receptors, and cyclooxygenase-2 pathway, since its main component, linoleic acid, has been demonstrated to produce such effect in previous studies.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Neuroquímica Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal do Pará (UFPA), Rua Augusto Correa s/n, Guamá, 66075-900 Belém, PA, Brazil ; Laboratório de Neuroinflamação, Instituto de Ciências Biológicas, Universidade Federal do Pará (UFPA), Rua Augusto Correa s/n, Guamá, 66075-900 Belém, PA, Brazil.

ABSTRACT
Libidibia ferrea (LF) is a medicinal plant that holds many pharmacological properties. We evaluated the antinociceptive effect in the LF aqueous seed extract and Lipidic Portion of Libidibia ferrea (LPLF), partially elucidating their mechanisms. Histochemical tests and Gas chromatography of the LPLF were performed to characterize its fatty acids. Acetic acid-induced abdominal constriction, formalin-induced pain, and hot-plate test in mice were employed in the study. In all experiments, aqueous extract or LPLF was administered systemically at the doses of 1, 5, and 10 mg/kg. LF aqueous seed extract and LPLF demonstrated a dose-dependent antinociceptive effect in all tests indicating both peripheral anti-inflammatory and central analgesia properties. Also, the use of atropine (5 mg/kg), naloxone (5 mg/kg) in the abdominal writhing test was able to reverse the antinociceptive effect of the LPLF, indicating that at least one of LF lipids components is responsible for the dose related antinociceptive action in chemical and thermal models of nociception in mice. Together, the present results suggested that Libidibia ferrea induced antinociceptive activity is possibly related to its ability to inhibit opioid, cholinergic receptors, and cyclooxygenase-2 pathway, since its main component, linoleic acid, has been demonstrated to produce such effect in previous studies.

Show MeSH
Related in: MedlinePlus