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Libidibia ferrea mature seeds promote antinociceptive effect by peripheral and central pathway: possible involvement of opioid and cholinergic receptors.

Sawada LA, Monteiro VS, Rabelo GR, Dias GB, Da Cunha M, do Nascimento JL, Bastos Gde N - Biomed Res Int (2014)

Bottom Line: We evaluated the antinociceptive effect in the LF aqueous seed extract and Lipidic Portion of Libidibia ferrea (LPLF), partially elucidating their mechanisms.LF aqueous seed extract and LPLF demonstrated a dose-dependent antinociceptive effect in all tests indicating both peripheral anti-inflammatory and central analgesia properties.Together, the present results suggested that Libidibia ferrea induced antinociceptive activity is possibly related to its ability to inhibit opioid, cholinergic receptors, and cyclooxygenase-2 pathway, since its main component, linoleic acid, has been demonstrated to produce such effect in previous studies.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Neuroquímica Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal do Pará (UFPA), Rua Augusto Correa s/n, Guamá, 66075-900 Belém, PA, Brazil ; Laboratório de Neuroinflamação, Instituto de Ciências Biológicas, Universidade Federal do Pará (UFPA), Rua Augusto Correa s/n, Guamá, 66075-900 Belém, PA, Brazil.

ABSTRACT
Libidibia ferrea (LF) is a medicinal plant that holds many pharmacological properties. We evaluated the antinociceptive effect in the LF aqueous seed extract and Lipidic Portion of Libidibia ferrea (LPLF), partially elucidating their mechanisms. Histochemical tests and Gas chromatography of the LPLF were performed to characterize its fatty acids. Acetic acid-induced abdominal constriction, formalin-induced pain, and hot-plate test in mice were employed in the study. In all experiments, aqueous extract or LPLF was administered systemically at the doses of 1, 5, and 10 mg/kg. LF aqueous seed extract and LPLF demonstrated a dose-dependent antinociceptive effect in all tests indicating both peripheral anti-inflammatory and central analgesia properties. Also, the use of atropine (5 mg/kg), naloxone (5 mg/kg) in the abdominal writhing test was able to reverse the antinociceptive effect of the LPLF, indicating that at least one of LF lipids components is responsible for the dose related antinociceptive action in chemical and thermal models of nociception in mice. Together, the present results suggested that Libidibia ferrea induced antinociceptive activity is possibly related to its ability to inhibit opioid, cholinergic receptors, and cyclooxygenase-2 pathway, since its main component, linoleic acid, has been demonstrated to produce such effect in previous studies.

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Histochemical tests in the longitudinal section of seeds of Libidibia ferrea. (a) Seed control showing the organization of main issues. (b) Sudan IV test that revealed the presence of lipids in the cotyledon and (c) in the seed coat cuticle. (d) Osmium tetroxide test showed that the lipids are mainly unsaturated and predominantly observed in the cotyledon, (e) in the basal portion of macrosclereids, and (f) in the fibers. (g-h) Nile blue sulphate test reveals the presence of neutral and acids lipids in the cuticle epidermis (open triangle) of cotyledons and (i) mainly in the subcuticular space (asterisks). (j) NADI reagent also revealed the presence of terpenoid only in the epidermal cells of cotyledons (arrows). ms: macrosclereid; os: osteosclereids; fi: fibers; pa: parenchyma; co: cotyledon; ep: epidermis of cotyledon; se: subepidermis of cotyledon; sc: subcuticular space. Scale bar: (a, g) = 200 μm; (b-c, j) = 20 μm; (d-e) = 100 μm; (f, i) = 50 μm; (h) = 25 μm; (j) = 40 μm.
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fig2: Histochemical tests in the longitudinal section of seeds of Libidibia ferrea. (a) Seed control showing the organization of main issues. (b) Sudan IV test that revealed the presence of lipids in the cotyledon and (c) in the seed coat cuticle. (d) Osmium tetroxide test showed that the lipids are mainly unsaturated and predominantly observed in the cotyledon, (e) in the basal portion of macrosclereids, and (f) in the fibers. (g-h) Nile blue sulphate test reveals the presence of neutral and acids lipids in the cuticle epidermis (open triangle) of cotyledons and (i) mainly in the subcuticular space (asterisks). (j) NADI reagent also revealed the presence of terpenoid only in the epidermal cells of cotyledons (arrows). ms: macrosclereid; os: osteosclereids; fi: fibers; pa: parenchyma; co: cotyledon; ep: epidermis of cotyledon; se: subepidermis of cotyledon; sc: subcuticular space. Scale bar: (a, g) = 200 μm; (b-c, j) = 20 μm; (d-e) = 100 μm; (f, i) = 50 μm; (h) = 25 μm; (j) = 40 μm.

Mentions: Libidibia ferrea presented unitegumented seed composed of exotest epidermis with a thin cuticle layer; a subcuticular space and a palisade layer were formed by macrosclereids. A mesotest composed of osteosclereids and several layers of fibers, and an endotest composed of an inner surface; the space between the exotest and the cotyledon was occupied for endosperm (Figure 2(a)).


Libidibia ferrea mature seeds promote antinociceptive effect by peripheral and central pathway: possible involvement of opioid and cholinergic receptors.

Sawada LA, Monteiro VS, Rabelo GR, Dias GB, Da Cunha M, do Nascimento JL, Bastos Gde N - Biomed Res Int (2014)

Histochemical tests in the longitudinal section of seeds of Libidibia ferrea. (a) Seed control showing the organization of main issues. (b) Sudan IV test that revealed the presence of lipids in the cotyledon and (c) in the seed coat cuticle. (d) Osmium tetroxide test showed that the lipids are mainly unsaturated and predominantly observed in the cotyledon, (e) in the basal portion of macrosclereids, and (f) in the fibers. (g-h) Nile blue sulphate test reveals the presence of neutral and acids lipids in the cuticle epidermis (open triangle) of cotyledons and (i) mainly in the subcuticular space (asterisks). (j) NADI reagent also revealed the presence of terpenoid only in the epidermal cells of cotyledons (arrows). ms: macrosclereid; os: osteosclereids; fi: fibers; pa: parenchyma; co: cotyledon; ep: epidermis of cotyledon; se: subepidermis of cotyledon; sc: subcuticular space. Scale bar: (a, g) = 200 μm; (b-c, j) = 20 μm; (d-e) = 100 μm; (f, i) = 50 μm; (h) = 25 μm; (j) = 40 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016940&req=5

fig2: Histochemical tests in the longitudinal section of seeds of Libidibia ferrea. (a) Seed control showing the organization of main issues. (b) Sudan IV test that revealed the presence of lipids in the cotyledon and (c) in the seed coat cuticle. (d) Osmium tetroxide test showed that the lipids are mainly unsaturated and predominantly observed in the cotyledon, (e) in the basal portion of macrosclereids, and (f) in the fibers. (g-h) Nile blue sulphate test reveals the presence of neutral and acids lipids in the cuticle epidermis (open triangle) of cotyledons and (i) mainly in the subcuticular space (asterisks). (j) NADI reagent also revealed the presence of terpenoid only in the epidermal cells of cotyledons (arrows). ms: macrosclereid; os: osteosclereids; fi: fibers; pa: parenchyma; co: cotyledon; ep: epidermis of cotyledon; se: subepidermis of cotyledon; sc: subcuticular space. Scale bar: (a, g) = 200 μm; (b-c, j) = 20 μm; (d-e) = 100 μm; (f, i) = 50 μm; (h) = 25 μm; (j) = 40 μm.
Mentions: Libidibia ferrea presented unitegumented seed composed of exotest epidermis with a thin cuticle layer; a subcuticular space and a palisade layer were formed by macrosclereids. A mesotest composed of osteosclereids and several layers of fibers, and an endotest composed of an inner surface; the space between the exotest and the cotyledon was occupied for endosperm (Figure 2(a)).

Bottom Line: We evaluated the antinociceptive effect in the LF aqueous seed extract and Lipidic Portion of Libidibia ferrea (LPLF), partially elucidating their mechanisms.LF aqueous seed extract and LPLF demonstrated a dose-dependent antinociceptive effect in all tests indicating both peripheral anti-inflammatory and central analgesia properties.Together, the present results suggested that Libidibia ferrea induced antinociceptive activity is possibly related to its ability to inhibit opioid, cholinergic receptors, and cyclooxygenase-2 pathway, since its main component, linoleic acid, has been demonstrated to produce such effect in previous studies.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Neuroquímica Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal do Pará (UFPA), Rua Augusto Correa s/n, Guamá, 66075-900 Belém, PA, Brazil ; Laboratório de Neuroinflamação, Instituto de Ciências Biológicas, Universidade Federal do Pará (UFPA), Rua Augusto Correa s/n, Guamá, 66075-900 Belém, PA, Brazil.

ABSTRACT
Libidibia ferrea (LF) is a medicinal plant that holds many pharmacological properties. We evaluated the antinociceptive effect in the LF aqueous seed extract and Lipidic Portion of Libidibia ferrea (LPLF), partially elucidating their mechanisms. Histochemical tests and Gas chromatography of the LPLF were performed to characterize its fatty acids. Acetic acid-induced abdominal constriction, formalin-induced pain, and hot-plate test in mice were employed in the study. In all experiments, aqueous extract or LPLF was administered systemically at the doses of 1, 5, and 10 mg/kg. LF aqueous seed extract and LPLF demonstrated a dose-dependent antinociceptive effect in all tests indicating both peripheral anti-inflammatory and central analgesia properties. Also, the use of atropine (5 mg/kg), naloxone (5 mg/kg) in the abdominal writhing test was able to reverse the antinociceptive effect of the LPLF, indicating that at least one of LF lipids components is responsible for the dose related antinociceptive action in chemical and thermal models of nociception in mice. Together, the present results suggested that Libidibia ferrea induced antinociceptive activity is possibly related to its ability to inhibit opioid, cholinergic receptors, and cyclooxygenase-2 pathway, since its main component, linoleic acid, has been demonstrated to produce such effect in previous studies.

Show MeSH
Related in: MedlinePlus