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Propolis Reduces Phosphatidylcholine-Specific Phospholipase C Activity and Increases Annexin a7 Level in Oxidized-LDL-Stimulated Human Umbilical Vein Endothelial Cells.

Xuan H, Li Z, Wang J, Wang K, Fu C, Yuan J, Hu F - Evid Based Complement Alternat Med (2014)

Bottom Line: To understand the mechanisms underlying the regulating dyslipidemia action of Chinese propolis and Brazilian green propolis, we investigated their effects on phosphatidylcholine-specific phospholipase C (PC-PLC) activity and annexin a7 (ANXA7) level which play crucial roles in the control of the progress of atherosclerosis.Our data indicated that the treatment of both types of propolis 12.5  μ g/mL significantly increased cell viability and attenuated apoptosis rate, increased ANXA7 level, and decreased PC-PLC activity.Our results also indicated that Chinese propolis and Brazilian green propolis had similar biological activities and prevented ox-LDL induced cellular dysfunction in HUVECs.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, China ; College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.

ABSTRACT
To understand the mechanisms underlying the regulating dyslipidemia action of Chinese propolis and Brazilian green propolis, we investigated their effects on phosphatidylcholine-specific phospholipase C (PC-PLC) activity and annexin a7 (ANXA7) level which play crucial roles in the control of the progress of atherosclerosis. Furthermore, active oxygen species (ROS) levels, nuclear factor-KappaB p65 (NF- κ B p65), and mitochondrial membrane potential (MMP) were also investigated in oxidized-LDL- (ox-LDL-) stimulated human umbilical vein endothelial cells (HUVECs). Our data indicated that the treatment of both types of propolis 12.5  μ g/mL significantly increased cell viability and attenuated apoptosis rate, increased ANXA7 level, and decreased PC-PLC activity. Both types of propolis also inhibited ROS generation as well as the subsequent MMP collapse, and NF- κ B p65 activation induced by ox-LDL in HUVECs. Our results also indicated that Chinese propolis and Brazilian green propolis had similar biological activities and prevented ox-LDL induced cellular dysfunction in HUVECs.

No MeSH data available.


Related in: MedlinePlus

EECP and EEBP increased ANXA7 level in ox-LDL-stimulated HUVECs. (a) Fluorescent micrographs obtained at 24 h. (b) ANXA7 levels were detected by western blot analysis at 12 and 24 h. (c) The hemiquantification of ANXA7 level in HUVECs (**P < 0.01 versus ox-LDL group, n = 3).
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fig3: EECP and EEBP increased ANXA7 level in ox-LDL-stimulated HUVECs. (a) Fluorescent micrographs obtained at 24 h. (b) ANXA7 levels were detected by western blot analysis at 12 and 24 h. (c) The hemiquantification of ANXA7 level in HUVECs (**P < 0.01 versus ox-LDL group, n = 3).

Mentions: ANXA7 was the endogenous regulator of PC-PLC. To further investigate the relationship between PC-PLC and ANXA7, we investigated the effect of EECP and EEBP on ANXA7 expression and distribution in cells treated with ox-LDL. Western blotting results showed that EEBP obviously increased ANXA7 level at 12 h, and both EECP and EEBP significantly increased ANXA7 level at 24 h (**P < 0.01; Figure 3). And immunofluorescence assay results showed that cells treated with EECP and EEBP exhibited higher fluorescence intensity of ANXA7 per cell in a noticeable punctate pattern compared with ox-LDL group (Figure 3(a)).


Propolis Reduces Phosphatidylcholine-Specific Phospholipase C Activity and Increases Annexin a7 Level in Oxidized-LDL-Stimulated Human Umbilical Vein Endothelial Cells.

Xuan H, Li Z, Wang J, Wang K, Fu C, Yuan J, Hu F - Evid Based Complement Alternat Med (2014)

EECP and EEBP increased ANXA7 level in ox-LDL-stimulated HUVECs. (a) Fluorescent micrographs obtained at 24 h. (b) ANXA7 levels were detected by western blot analysis at 12 and 24 h. (c) The hemiquantification of ANXA7 level in HUVECs (**P < 0.01 versus ox-LDL group, n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016937&req=5

fig3: EECP and EEBP increased ANXA7 level in ox-LDL-stimulated HUVECs. (a) Fluorescent micrographs obtained at 24 h. (b) ANXA7 levels were detected by western blot analysis at 12 and 24 h. (c) The hemiquantification of ANXA7 level in HUVECs (**P < 0.01 versus ox-LDL group, n = 3).
Mentions: ANXA7 was the endogenous regulator of PC-PLC. To further investigate the relationship between PC-PLC and ANXA7, we investigated the effect of EECP and EEBP on ANXA7 expression and distribution in cells treated with ox-LDL. Western blotting results showed that EEBP obviously increased ANXA7 level at 12 h, and both EECP and EEBP significantly increased ANXA7 level at 24 h (**P < 0.01; Figure 3). And immunofluorescence assay results showed that cells treated with EECP and EEBP exhibited higher fluorescence intensity of ANXA7 per cell in a noticeable punctate pattern compared with ox-LDL group (Figure 3(a)).

Bottom Line: To understand the mechanisms underlying the regulating dyslipidemia action of Chinese propolis and Brazilian green propolis, we investigated their effects on phosphatidylcholine-specific phospholipase C (PC-PLC) activity and annexin a7 (ANXA7) level which play crucial roles in the control of the progress of atherosclerosis.Our data indicated that the treatment of both types of propolis 12.5  μ g/mL significantly increased cell viability and attenuated apoptosis rate, increased ANXA7 level, and decreased PC-PLC activity.Our results also indicated that Chinese propolis and Brazilian green propolis had similar biological activities and prevented ox-LDL induced cellular dysfunction in HUVECs.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, China ; College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.

ABSTRACT
To understand the mechanisms underlying the regulating dyslipidemia action of Chinese propolis and Brazilian green propolis, we investigated their effects on phosphatidylcholine-specific phospholipase C (PC-PLC) activity and annexin a7 (ANXA7) level which play crucial roles in the control of the progress of atherosclerosis. Furthermore, active oxygen species (ROS) levels, nuclear factor-KappaB p65 (NF- κ B p65), and mitochondrial membrane potential (MMP) were also investigated in oxidized-LDL- (ox-LDL-) stimulated human umbilical vein endothelial cells (HUVECs). Our data indicated that the treatment of both types of propolis 12.5  μ g/mL significantly increased cell viability and attenuated apoptosis rate, increased ANXA7 level, and decreased PC-PLC activity. Both types of propolis also inhibited ROS generation as well as the subsequent MMP collapse, and NF- κ B p65 activation induced by ox-LDL in HUVECs. Our results also indicated that Chinese propolis and Brazilian green propolis had similar biological activities and prevented ox-LDL induced cellular dysfunction in HUVECs.

No MeSH data available.


Related in: MedlinePlus