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Propolis Reduces Phosphatidylcholine-Specific Phospholipase C Activity and Increases Annexin a7 Level in Oxidized-LDL-Stimulated Human Umbilical Vein Endothelial Cells.

Xuan H, Li Z, Wang J, Wang K, Fu C, Yuan J, Hu F - Evid Based Complement Alternat Med (2014)

Bottom Line: To understand the mechanisms underlying the regulating dyslipidemia action of Chinese propolis and Brazilian green propolis, we investigated their effects on phosphatidylcholine-specific phospholipase C (PC-PLC) activity and annexin a7 (ANXA7) level which play crucial roles in the control of the progress of atherosclerosis.Our data indicated that the treatment of both types of propolis 12.5  μ g/mL significantly increased cell viability and attenuated apoptosis rate, increased ANXA7 level, and decreased PC-PLC activity.Our results also indicated that Chinese propolis and Brazilian green propolis had similar biological activities and prevented ox-LDL induced cellular dysfunction in HUVECs.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, China ; College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.

ABSTRACT
To understand the mechanisms underlying the regulating dyslipidemia action of Chinese propolis and Brazilian green propolis, we investigated their effects on phosphatidylcholine-specific phospholipase C (PC-PLC) activity and annexin a7 (ANXA7) level which play crucial roles in the control of the progress of atherosclerosis. Furthermore, active oxygen species (ROS) levels, nuclear factor-KappaB p65 (NF- κ B p65), and mitochondrial membrane potential (MMP) were also investigated in oxidized-LDL- (ox-LDL-) stimulated human umbilical vein endothelial cells (HUVECs). Our data indicated that the treatment of both types of propolis 12.5  μ g/mL significantly increased cell viability and attenuated apoptosis rate, increased ANXA7 level, and decreased PC-PLC activity. Both types of propolis also inhibited ROS generation as well as the subsequent MMP collapse, and NF- κ B p65 activation induced by ox-LDL in HUVECs. Our results also indicated that Chinese propolis and Brazilian green propolis had similar biological activities and prevented ox-LDL induced cellular dysfunction in HUVECs.

No MeSH data available.


Related in: MedlinePlus

EECP and EEBP decreased PC-PLC activity in ox-LDL-stimulated HUVECs. Cells were treated with EECP and EEBP 12.5 μg/mL for 24 h, respectively (*P < 0.05, **P < 0.01 versus ox-LDL group, n = 3).
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fig2: EECP and EEBP decreased PC-PLC activity in ox-LDL-stimulated HUVECs. Cells were treated with EECP and EEBP 12.5 μg/mL for 24 h, respectively (*P < 0.05, **P < 0.01 versus ox-LDL group, n = 3).

Mentions: The activity of PC-PLC in ox-LDL treated HUVECs was significantly increased, whereas EECP and EEBP evidently depressed PC-PLC activity at 24 h (*P < 0.05, **P < 0.01; Figure 2).


Propolis Reduces Phosphatidylcholine-Specific Phospholipase C Activity and Increases Annexin a7 Level in Oxidized-LDL-Stimulated Human Umbilical Vein Endothelial Cells.

Xuan H, Li Z, Wang J, Wang K, Fu C, Yuan J, Hu F - Evid Based Complement Alternat Med (2014)

EECP and EEBP decreased PC-PLC activity in ox-LDL-stimulated HUVECs. Cells were treated with EECP and EEBP 12.5 μg/mL for 24 h, respectively (*P < 0.05, **P < 0.01 versus ox-LDL group, n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016937&req=5

fig2: EECP and EEBP decreased PC-PLC activity in ox-LDL-stimulated HUVECs. Cells were treated with EECP and EEBP 12.5 μg/mL for 24 h, respectively (*P < 0.05, **P < 0.01 versus ox-LDL group, n = 3).
Mentions: The activity of PC-PLC in ox-LDL treated HUVECs was significantly increased, whereas EECP and EEBP evidently depressed PC-PLC activity at 24 h (*P < 0.05, **P < 0.01; Figure 2).

Bottom Line: To understand the mechanisms underlying the regulating dyslipidemia action of Chinese propolis and Brazilian green propolis, we investigated their effects on phosphatidylcholine-specific phospholipase C (PC-PLC) activity and annexin a7 (ANXA7) level which play crucial roles in the control of the progress of atherosclerosis.Our data indicated that the treatment of both types of propolis 12.5  μ g/mL significantly increased cell viability and attenuated apoptosis rate, increased ANXA7 level, and decreased PC-PLC activity.Our results also indicated that Chinese propolis and Brazilian green propolis had similar biological activities and prevented ox-LDL induced cellular dysfunction in HUVECs.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, China ; College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.

ABSTRACT
To understand the mechanisms underlying the regulating dyslipidemia action of Chinese propolis and Brazilian green propolis, we investigated their effects on phosphatidylcholine-specific phospholipase C (PC-PLC) activity and annexin a7 (ANXA7) level which play crucial roles in the control of the progress of atherosclerosis. Furthermore, active oxygen species (ROS) levels, nuclear factor-KappaB p65 (NF- κ B p65), and mitochondrial membrane potential (MMP) were also investigated in oxidized-LDL- (ox-LDL-) stimulated human umbilical vein endothelial cells (HUVECs). Our data indicated that the treatment of both types of propolis 12.5  μ g/mL significantly increased cell viability and attenuated apoptosis rate, increased ANXA7 level, and decreased PC-PLC activity. Both types of propolis also inhibited ROS generation as well as the subsequent MMP collapse, and NF- κ B p65 activation induced by ox-LDL in HUVECs. Our results also indicated that Chinese propolis and Brazilian green propolis had similar biological activities and prevented ox-LDL induced cellular dysfunction in HUVECs.

No MeSH data available.


Related in: MedlinePlus