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Expression and clinical significance of YAP, TAZ, and AREG in hepatocellular carcinoma.

Han SX, Bai E, Jin GH, He CC, Guo XJ, Wang LJ, Li M, Ying X, Zhu Q - J Immunol Res (2014)

Bottom Line: The expression of YAP was significantly correlated with Edmondson stage (P>0.05), serum AFP level (P>0.05), and HCC prognosis (P>0.05).AREG expression was also significantly correlated with Edmondson stage (P>0.05) and serum AFP level (P>0.05).Further multivariate analysis showed that YAP expression was an independent prognostic factor that significantly affected the overall survival of HCC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University Medical College, Xi'an, Shaanxi 710061, China.

ABSTRACT

Purpose: Yes-associated protein (YAP) and PDZ-binding motif (TAZ) are two important effectors of Hippo pathway controlling the balance of organ size and carcinogenesis. Amphiregulin (AREG) is a member of the epidermal growth factor family, a direct target gene of YAP and TAZ. The role of these proteins in hepatocellular carcinoma (HCC) is unclear.

Methods: The expression of YAP, TAZ, and AREG in HCC was analyzed by immunohistochemical staining. The level of secreted serum AREG was also assayed by enzyme-linked immunosorbent (ELISA) assay.

Results: YAP, TAZ, and AREG were expressed in 69.2% (27/39), 66.7% (26/39), and 61.5% (24/39) of HCC patients. The expression of YAP was significantly correlated with Edmondson stage (P>0.05), serum AFP level (P>0.05), and HCC prognosis (P>0.05). AREG expression was also significantly correlated with Edmondson stage (P>0.05) and serum AFP level (P>0.05). In addition, the expression of serum AREG was higher than serum AFP in HCC patients. Further multivariate analysis showed that YAP expression was an independent prognostic factor that significantly affected the overall survival of HCC patients.

Conclusions: YAP maybe an independent prognostic indicator for HCC patients and serum AREG may be a serological biomarker of HCC.

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Related in: MedlinePlus

(a) showed the levels of serum AREG among normal controls, benign liver disease, and HCC patients. Mean levels of serum AREG and Std. Deviation is illustrated by bar charts; (b) showed the ROC curves of serum AREG between normal controls and HCC patients.
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fig3: (a) showed the levels of serum AREG among normal controls, benign liver disease, and HCC patients. Mean levels of serum AREG and Std. Deviation is illustrated by bar charts; (b) showed the ROC curves of serum AREG between normal controls and HCC patients.

Mentions: The expressions of serum AREG in 98 patients were determined by ELISA assay. The characteristics of 98 patients are summarized as shown in Table 4. We found serum AREG levels in HCC patients to be significantly higher than that of patients with benign liver disease and normal controls (P < 0.01). There was no significant difference between serum AREG levels of patients with benign liver disease and normal controls (P > 0.05, Figure 3(a)). Patients with HCC stages I-II did not have an elevated AREG compared to patients with HCC stages III-IV (P = 0.266).


Expression and clinical significance of YAP, TAZ, and AREG in hepatocellular carcinoma.

Han SX, Bai E, Jin GH, He CC, Guo XJ, Wang LJ, Li M, Ying X, Zhu Q - J Immunol Res (2014)

(a) showed the levels of serum AREG among normal controls, benign liver disease, and HCC patients. Mean levels of serum AREG and Std. Deviation is illustrated by bar charts; (b) showed the ROC curves of serum AREG between normal controls and HCC patients.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016924&req=5

fig3: (a) showed the levels of serum AREG among normal controls, benign liver disease, and HCC patients. Mean levels of serum AREG and Std. Deviation is illustrated by bar charts; (b) showed the ROC curves of serum AREG between normal controls and HCC patients.
Mentions: The expressions of serum AREG in 98 patients were determined by ELISA assay. The characteristics of 98 patients are summarized as shown in Table 4. We found serum AREG levels in HCC patients to be significantly higher than that of patients with benign liver disease and normal controls (P < 0.01). There was no significant difference between serum AREG levels of patients with benign liver disease and normal controls (P > 0.05, Figure 3(a)). Patients with HCC stages I-II did not have an elevated AREG compared to patients with HCC stages III-IV (P = 0.266).

Bottom Line: The expression of YAP was significantly correlated with Edmondson stage (P>0.05), serum AFP level (P>0.05), and HCC prognosis (P>0.05).AREG expression was also significantly correlated with Edmondson stage (P>0.05) and serum AFP level (P>0.05).Further multivariate analysis showed that YAP expression was an independent prognostic factor that significantly affected the overall survival of HCC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University Medical College, Xi'an, Shaanxi 710061, China.

ABSTRACT

Purpose: Yes-associated protein (YAP) and PDZ-binding motif (TAZ) are two important effectors of Hippo pathway controlling the balance of organ size and carcinogenesis. Amphiregulin (AREG) is a member of the epidermal growth factor family, a direct target gene of YAP and TAZ. The role of these proteins in hepatocellular carcinoma (HCC) is unclear.

Methods: The expression of YAP, TAZ, and AREG in HCC was analyzed by immunohistochemical staining. The level of secreted serum AREG was also assayed by enzyme-linked immunosorbent (ELISA) assay.

Results: YAP, TAZ, and AREG were expressed in 69.2% (27/39), 66.7% (26/39), and 61.5% (24/39) of HCC patients. The expression of YAP was significantly correlated with Edmondson stage (P>0.05), serum AFP level (P>0.05), and HCC prognosis (P>0.05). AREG expression was also significantly correlated with Edmondson stage (P>0.05) and serum AFP level (P>0.05). In addition, the expression of serum AREG was higher than serum AFP in HCC patients. Further multivariate analysis showed that YAP expression was an independent prognostic factor that significantly affected the overall survival of HCC patients.

Conclusions: YAP maybe an independent prognostic indicator for HCC patients and serum AREG may be a serological biomarker of HCC.

Show MeSH
Related in: MedlinePlus