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Expression and clinical significance of YAP, TAZ, and AREG in hepatocellular carcinoma.

Han SX, Bai E, Jin GH, He CC, Guo XJ, Wang LJ, Li M, Ying X, Zhu Q - J Immunol Res (2014)

Bottom Line: The expression of YAP was significantly correlated with Edmondson stage (P>0.05), serum AFP level (P>0.05), and HCC prognosis (P>0.05).AREG expression was also significantly correlated with Edmondson stage (P>0.05) and serum AFP level (P>0.05).Further multivariate analysis showed that YAP expression was an independent prognostic factor that significantly affected the overall survival of HCC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University Medical College, Xi'an, Shaanxi 710061, China.

ABSTRACT

Purpose: Yes-associated protein (YAP) and PDZ-binding motif (TAZ) are two important effectors of Hippo pathway controlling the balance of organ size and carcinogenesis. Amphiregulin (AREG) is a member of the epidermal growth factor family, a direct target gene of YAP and TAZ. The role of these proteins in hepatocellular carcinoma (HCC) is unclear.

Methods: The expression of YAP, TAZ, and AREG in HCC was analyzed by immunohistochemical staining. The level of secreted serum AREG was also assayed by enzyme-linked immunosorbent (ELISA) assay.

Results: YAP, TAZ, and AREG were expressed in 69.2% (27/39), 66.7% (26/39), and 61.5% (24/39) of HCC patients. The expression of YAP was significantly correlated with Edmondson stage (P>0.05), serum AFP level (P>0.05), and HCC prognosis (P>0.05). AREG expression was also significantly correlated with Edmondson stage (P>0.05) and serum AFP level (P>0.05). In addition, the expression of serum AREG was higher than serum AFP in HCC patients. Further multivariate analysis showed that YAP expression was an independent prognostic factor that significantly affected the overall survival of HCC patients.

Conclusions: YAP maybe an independent prognostic indicator for HCC patients and serum AREG may be a serological biomarker of HCC.

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Related in: MedlinePlus

Kaplan-Meier analysis of overall survival (cumulative survival) of HCC patients relative to YAP, TAZ, and AREG expression. (a) Correlation of YAP expression with overall survival of HCC patients; (b) correlation of TAZ expression with overall survival of HCC patients; (c) correlation of AREG expression with overall survival of HCC patients.
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fig2: Kaplan-Meier analysis of overall survival (cumulative survival) of HCC patients relative to YAP, TAZ, and AREG expression. (a) Correlation of YAP expression with overall survival of HCC patients; (b) correlation of TAZ expression with overall survival of HCC patients; (c) correlation of AREG expression with overall survival of HCC patients.

Mentions: To determine the prognostic significance of YAP and TAZ expression, we followed 39 HCC patients for two years. The overall median survival time among these patients was 19.5 months. At the end of the follow-up period 20 patients were deceased. The association between YAP and TAZ expression and overall survival of HCC patients was investigated by Kaplan-Meier analysis and log-rank test. A statistically significant difference was found between the overall survival and YAP protein expression (log-rank = 4.819, P = 0.028, as shown in Figure 2(a)). Univariate Cox proportional hazard analysis of protein expression and the relationship to overall survival are shown in Table 3. HCC patients with high expressions of YAP tended to have higher risk of death (P = 0.042). In addition, cell differentiation (Edmonson grade) and number of tumor nodules were found to be associated with HCC prognosis (as shown in Table 3). Multivariate analysis showed that higher expression of YAP was associated with reduced overall survival, adjusted HR 0.054 (P = 0.005). This indicates that the expression of YAP could be a prognostic factor independent of these adjusted clinicopathologic characteristics. On the other hand, the expression of TAZ (P = 0.722, Figure 2(b)) or AREG (P = 0.583, Figure 2(c)) did not predict overall survival of HCC patients.


Expression and clinical significance of YAP, TAZ, and AREG in hepatocellular carcinoma.

Han SX, Bai E, Jin GH, He CC, Guo XJ, Wang LJ, Li M, Ying X, Zhu Q - J Immunol Res (2014)

Kaplan-Meier analysis of overall survival (cumulative survival) of HCC patients relative to YAP, TAZ, and AREG expression. (a) Correlation of YAP expression with overall survival of HCC patients; (b) correlation of TAZ expression with overall survival of HCC patients; (c) correlation of AREG expression with overall survival of HCC patients.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016924&req=5

fig2: Kaplan-Meier analysis of overall survival (cumulative survival) of HCC patients relative to YAP, TAZ, and AREG expression. (a) Correlation of YAP expression with overall survival of HCC patients; (b) correlation of TAZ expression with overall survival of HCC patients; (c) correlation of AREG expression with overall survival of HCC patients.
Mentions: To determine the prognostic significance of YAP and TAZ expression, we followed 39 HCC patients for two years. The overall median survival time among these patients was 19.5 months. At the end of the follow-up period 20 patients were deceased. The association between YAP and TAZ expression and overall survival of HCC patients was investigated by Kaplan-Meier analysis and log-rank test. A statistically significant difference was found between the overall survival and YAP protein expression (log-rank = 4.819, P = 0.028, as shown in Figure 2(a)). Univariate Cox proportional hazard analysis of protein expression and the relationship to overall survival are shown in Table 3. HCC patients with high expressions of YAP tended to have higher risk of death (P = 0.042). In addition, cell differentiation (Edmonson grade) and number of tumor nodules were found to be associated with HCC prognosis (as shown in Table 3). Multivariate analysis showed that higher expression of YAP was associated with reduced overall survival, adjusted HR 0.054 (P = 0.005). This indicates that the expression of YAP could be a prognostic factor independent of these adjusted clinicopathologic characteristics. On the other hand, the expression of TAZ (P = 0.722, Figure 2(b)) or AREG (P = 0.583, Figure 2(c)) did not predict overall survival of HCC patients.

Bottom Line: The expression of YAP was significantly correlated with Edmondson stage (P>0.05), serum AFP level (P>0.05), and HCC prognosis (P>0.05).AREG expression was also significantly correlated with Edmondson stage (P>0.05) and serum AFP level (P>0.05).Further multivariate analysis showed that YAP expression was an independent prognostic factor that significantly affected the overall survival of HCC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University Medical College, Xi'an, Shaanxi 710061, China.

ABSTRACT

Purpose: Yes-associated protein (YAP) and PDZ-binding motif (TAZ) are two important effectors of Hippo pathway controlling the balance of organ size and carcinogenesis. Amphiregulin (AREG) is a member of the epidermal growth factor family, a direct target gene of YAP and TAZ. The role of these proteins in hepatocellular carcinoma (HCC) is unclear.

Methods: The expression of YAP, TAZ, and AREG in HCC was analyzed by immunohistochemical staining. The level of secreted serum AREG was also assayed by enzyme-linked immunosorbent (ELISA) assay.

Results: YAP, TAZ, and AREG were expressed in 69.2% (27/39), 66.7% (26/39), and 61.5% (24/39) of HCC patients. The expression of YAP was significantly correlated with Edmondson stage (P>0.05), serum AFP level (P>0.05), and HCC prognosis (P>0.05). AREG expression was also significantly correlated with Edmondson stage (P>0.05) and serum AFP level (P>0.05). In addition, the expression of serum AREG was higher than serum AFP in HCC patients. Further multivariate analysis showed that YAP expression was an independent prognostic factor that significantly affected the overall survival of HCC patients.

Conclusions: YAP maybe an independent prognostic indicator for HCC patients and serum AREG may be a serological biomarker of HCC.

Show MeSH
Related in: MedlinePlus