Limits...
Stability analysis of a high fibre yield and low lignin content "thick stem" mutant in tossa jute (Corchorus olitorius L.).

Mandal A, Datta AK - Biomed Res Int (2014)

Bottom Line: The objective is to assess the stability of mutant for its effective exploration for raising a new plant type in tossa jute for commercial exploitation and efficient breeding.Results indicate that "thick stem" mutant is stable across generations (2n = 14) with distinctive high seed and fibre yield and significantly low lignin content.Moreover, tensile strength of mutant fibre is significantly higher than control fibre and the trait is inversely related to fibre diameter.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany, Cytogenetics, Genetics and Plant Breeding Section, Kalyani University, Kalyani, West Bengal 741235, India.

ABSTRACT
A "thick stem" mutant of Corchorus olitorius L. was induced at M2 (0.50%, 4 h, EMS) and the true breeding mutant is assessed across generations (M5 to M7) considering morphometric traits as well as SEM analysis of pollen grains and raw jute fibres, stem anatomy, cytogenetical attributes, and lignin content in relation to control. Furthermore, single fibre diameter and tensile strength are also analysed. The objective is to assess the stability of mutant for its effective exploration for raising a new plant type in tossa jute for commercial exploitation and efficient breeding. The mutant trait is monogenic recessive to normal. Results indicate that "thick stem" mutant is stable across generations (2n = 14) with distinctive high seed and fibre yield and significantly low lignin content. Stem anatomy of the mutant shows significant enhancement in fibre zone, number of fibre pyramids and fibre bundles per pyramid, and diameter of fibre cell in relation to control. Moreover, tensile strength of mutant fibre is significantly higher than control fibre and the trait is inversely related to fibre diameter. However the mutant is associated with low germination frequency, poor seed viability, and high pollen sterility, which may be eliminated through mutational approach followed by rigorous selection and efficient breeding.

Show MeSH

Related in: MedlinePlus

Scanning electron micrographs of pollen grains ((a), (b)) and raw jute fibres ((c), (d)). ((a) and (c)) Corchorus olitorius. ((b) and (d)) “thick stem” mutant. Scale bar = 20 μm.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4016914&req=5

fig3: Scanning electron micrographs of pollen grains ((a), (b)) and raw jute fibres ((c), (d)). ((a) and (c)) Corchorus olitorius. ((b) and (d)) “thick stem” mutant. Scale bar = 20 μm.

Mentions: In both the plant types pollen grains were subprolate; tricolporate; medium sized (control: 42.0 μm ±1.6 × 36.0 μm ± 0.8; mutant: 32.0 μm ± 1.1 × 26.0 μm ±0.7); colpi long (control: 33.0 μm ± 1.1; mutant: 24.0 μm ± 0.24), extending up to poles, wide, and asymmetrical; pore diameter 5.62 μm  ±  0.3 (control) to 5.10 μm  ±  0.2 (mutant); exine thick, reticulate, reticulation not uniform in size, becoming smaller towards the colpi margin; lumen area ranges from 0.22–1.60 μm2 (control) to 0.18–1.32 μm2 (mutant), polygonal; muri 0.38 μm ± 0.15 (control) to 0.30 μm ± 0.01 (mutant) thick (Figures 3(a) and 3(b)).


Stability analysis of a high fibre yield and low lignin content "thick stem" mutant in tossa jute (Corchorus olitorius L.).

Mandal A, Datta AK - Biomed Res Int (2014)

Scanning electron micrographs of pollen grains ((a), (b)) and raw jute fibres ((c), (d)). ((a) and (c)) Corchorus olitorius. ((b) and (d)) “thick stem” mutant. Scale bar = 20 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016914&req=5

fig3: Scanning electron micrographs of pollen grains ((a), (b)) and raw jute fibres ((c), (d)). ((a) and (c)) Corchorus olitorius. ((b) and (d)) “thick stem” mutant. Scale bar = 20 μm.
Mentions: In both the plant types pollen grains were subprolate; tricolporate; medium sized (control: 42.0 μm ±1.6 × 36.0 μm ± 0.8; mutant: 32.0 μm ± 1.1 × 26.0 μm ±0.7); colpi long (control: 33.0 μm ± 1.1; mutant: 24.0 μm ± 0.24), extending up to poles, wide, and asymmetrical; pore diameter 5.62 μm  ±  0.3 (control) to 5.10 μm  ±  0.2 (mutant); exine thick, reticulate, reticulation not uniform in size, becoming smaller towards the colpi margin; lumen area ranges from 0.22–1.60 μm2 (control) to 0.18–1.32 μm2 (mutant), polygonal; muri 0.38 μm ± 0.15 (control) to 0.30 μm ± 0.01 (mutant) thick (Figures 3(a) and 3(b)).

Bottom Line: The objective is to assess the stability of mutant for its effective exploration for raising a new plant type in tossa jute for commercial exploitation and efficient breeding.Results indicate that "thick stem" mutant is stable across generations (2n = 14) with distinctive high seed and fibre yield and significantly low lignin content.Moreover, tensile strength of mutant fibre is significantly higher than control fibre and the trait is inversely related to fibre diameter.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany, Cytogenetics, Genetics and Plant Breeding Section, Kalyani University, Kalyani, West Bengal 741235, India.

ABSTRACT
A "thick stem" mutant of Corchorus olitorius L. was induced at M2 (0.50%, 4 h, EMS) and the true breeding mutant is assessed across generations (M5 to M7) considering morphometric traits as well as SEM analysis of pollen grains and raw jute fibres, stem anatomy, cytogenetical attributes, and lignin content in relation to control. Furthermore, single fibre diameter and tensile strength are also analysed. The objective is to assess the stability of mutant for its effective exploration for raising a new plant type in tossa jute for commercial exploitation and efficient breeding. The mutant trait is monogenic recessive to normal. Results indicate that "thick stem" mutant is stable across generations (2n = 14) with distinctive high seed and fibre yield and significantly low lignin content. Stem anatomy of the mutant shows significant enhancement in fibre zone, number of fibre pyramids and fibre bundles per pyramid, and diameter of fibre cell in relation to control. Moreover, tensile strength of mutant fibre is significantly higher than control fibre and the trait is inversely related to fibre diameter. However the mutant is associated with low germination frequency, poor seed viability, and high pollen sterility, which may be eliminated through mutational approach followed by rigorous selection and efficient breeding.

Show MeSH
Related in: MedlinePlus