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Effects of cyhalothrin-based pesticide on early life stages of common carp (Cyprinus carpio L.).

Richterová Z, Máchová J, Stará A, Tumová J, Velíšek J, Sevčíková M, Svobodová Z - Biomed Res Int (2014)

Bottom Line: The effects of Nexide (a.i. gamma-cyhalothrin 60 g L(-1)) on cumulative mortality, growth indices, and ontogenetic development of embryos and larvae of common carp (Cyprinus carpio L.) were studied.Larvae exposed to 5  μ g L(-1) showed significantly lower growth and retarded ontogenetic development compared to control.The value of detoxification enzyme GST of organisms from the exposed group was significantly higher compared to the control and the value of defensive enzyme GPx was significantly lower compared to the control.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25 Vodnany, Czech Republic.

ABSTRACT
The effects of Nexide (a.i. gamma-cyhalothrin 60 g L(-1)) on cumulative mortality, growth indices, and ontogenetic development of embryos and larvae of common carp (Cyprinus carpio L.) were studied. Levels of oxidative stress parameters glutathione reductase (GR), glutathione peroxidase (GPx), catalase (CAT), glutathione-S-transferase (GST), and lipid peroxidation were determined. Eggs of newly fertilised common carp were exposed to Nexide at concentrations 5, 25, 50, 100, and 250  μ g L(-1) (0.3, 1.5, 3, 6, and 15  μ g L(-1) gamma-cyhalothrin). All organisms exposed to concentrations higher than 50  μ g L(-1) died soon after hatching; at 25  μ g L(-1), 95% mortality was recorded. Larvae exposed to 5  μ g L(-1) showed significantly lower growth and retarded ontogenetic development compared to control. Histological examination of the livers of larvae from the exposed group revealed dystrophic changes. The value of detoxification enzyme GST of organisms from the exposed group was significantly higher compared to the control and the value of defensive enzyme GPx was significantly lower compared to the control. The results of our investigation confirmed that contamination of aquatic environment by pesticides containing cyhalothrin may impair growth and development of early life stages of carp and cause disbalance of defensive enzymes.

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Effect of Nexide on total length (mean ± SD) of common carp larvae and juveniles during embryo-larval test. Significant differences (P < 0.05 on days 5–26 and P < 0.01 on days 33 and 35) between groups at each sampling time are indicated by different letters (a, b).
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fig2: Effect of Nexide on total length (mean ± SD) of common carp larvae and juveniles during embryo-larval test. Significant differences (P < 0.05 on days 5–26 and P < 0.01 on days 33 and 35) between groups at each sampling time are indicated by different letters (a, b).

Mentions: Growth in total length and weight and ontogenetic developmental stage are recorded (Figures 2 and 3). Samples from all concentrations were taken only on day 5, because no larvae survived beyond day 11 in 50, 100, and 250 μg L−1 exposure. Only the control and the 5 μg L−1 group were compared at the completion of the trial. Nineteen larvae/juvenile fish from the control group and ten larvae exposed to 5 μg L−1 were examined on day 35. Insufficient individuals survived in the 25 μg L−1 group to be included in growth comparisons.


Effects of cyhalothrin-based pesticide on early life stages of common carp (Cyprinus carpio L.).

Richterová Z, Máchová J, Stará A, Tumová J, Velíšek J, Sevčíková M, Svobodová Z - Biomed Res Int (2014)

Effect of Nexide on total length (mean ± SD) of common carp larvae and juveniles during embryo-larval test. Significant differences (P < 0.05 on days 5–26 and P < 0.01 on days 33 and 35) between groups at each sampling time are indicated by different letters (a, b).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016908&req=5

fig2: Effect of Nexide on total length (mean ± SD) of common carp larvae and juveniles during embryo-larval test. Significant differences (P < 0.05 on days 5–26 and P < 0.01 on days 33 and 35) between groups at each sampling time are indicated by different letters (a, b).
Mentions: Growth in total length and weight and ontogenetic developmental stage are recorded (Figures 2 and 3). Samples from all concentrations were taken only on day 5, because no larvae survived beyond day 11 in 50, 100, and 250 μg L−1 exposure. Only the control and the 5 μg L−1 group were compared at the completion of the trial. Nineteen larvae/juvenile fish from the control group and ten larvae exposed to 5 μg L−1 were examined on day 35. Insufficient individuals survived in the 25 μg L−1 group to be included in growth comparisons.

Bottom Line: The effects of Nexide (a.i. gamma-cyhalothrin 60 g L(-1)) on cumulative mortality, growth indices, and ontogenetic development of embryos and larvae of common carp (Cyprinus carpio L.) were studied.Larvae exposed to 5  μ g L(-1) showed significantly lower growth and retarded ontogenetic development compared to control.The value of detoxification enzyme GST of organisms from the exposed group was significantly higher compared to the control and the value of defensive enzyme GPx was significantly lower compared to the control.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25 Vodnany, Czech Republic.

ABSTRACT
The effects of Nexide (a.i. gamma-cyhalothrin 60 g L(-1)) on cumulative mortality, growth indices, and ontogenetic development of embryos and larvae of common carp (Cyprinus carpio L.) were studied. Levels of oxidative stress parameters glutathione reductase (GR), glutathione peroxidase (GPx), catalase (CAT), glutathione-S-transferase (GST), and lipid peroxidation were determined. Eggs of newly fertilised common carp were exposed to Nexide at concentrations 5, 25, 50, 100, and 250  μ g L(-1) (0.3, 1.5, 3, 6, and 15  μ g L(-1) gamma-cyhalothrin). All organisms exposed to concentrations higher than 50  μ g L(-1) died soon after hatching; at 25  μ g L(-1), 95% mortality was recorded. Larvae exposed to 5  μ g L(-1) showed significantly lower growth and retarded ontogenetic development compared to control. Histological examination of the livers of larvae from the exposed group revealed dystrophic changes. The value of detoxification enzyme GST of organisms from the exposed group was significantly higher compared to the control and the value of defensive enzyme GPx was significantly lower compared to the control. The results of our investigation confirmed that contamination of aquatic environment by pesticides containing cyhalothrin may impair growth and development of early life stages of carp and cause disbalance of defensive enzymes.

Show MeSH
Related in: MedlinePlus