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Chemokine CXCL16 expression suppresses migration and invasiveness and induces apoptosis in breast cancer cells.

Fang Y, Henderson FC, Yi Q, Lei Q, Li Y, Chen N - Mediators Inflamm. (2014)

Bottom Line: Increasing evidence argues that soluble CXCL16 promotes proliferation, migration, and invasion of cancer cells in vitro.Overexpression of CXCL16 in MDA-MB-231 cells led to a decrease in cell invasion and migration and induced apoptosis of the cells; downregulation of CXCL16 in MCF-7 cells increased cell migration and invasiveness.Consistent with the in vitro data, CXCL16 overexpression inhibited tumorigenesis in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 37 Guoxuexiang, Chengdu, Sichuan 610041, China.

ABSTRACT

Background: Increasing evidence argues that soluble CXCL16 promotes proliferation, migration, and invasion of cancer cells in vitro. However, the role of transmembrane or cellular CXCL16 in cancer remains relatively unknown. In this study, we determine the function of cellular CXCL16 as tumor suppressor in breast cancer cells.

Methods: Expression of cellular CXCL16 in breast cancer cell lines was determined at both RNA and protein levels. In vitro and in vivo studies that overexpressed or downregulated CXCL16 were conducted in breast cancer cells.

Results: We report differential expression of cellular CXCL16 in breast cancer cell lines that was negatively correlated with cell invasiveness and migration. Overexpression of CXCL16 in MDA-MB-231 cells led to a decrease in cell invasion and migration and induced apoptosis of the cells; downregulation of CXCL16 in MCF-7 cells increased cell migration and invasiveness. Consistent with the in vitro data, CXCL16 overexpression inhibited tumorigenesis in vivo.

Conclusions: Cellular CXCL16 suppresses invasion and metastasis of breast cancer cells in vitro and inhibits tumorigenesis in vivo. Targeting of cellular CXCL16 expression is a potential therapeutic strategy for breast cancer.

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Related in: MedlinePlus

CXCL16 expression inhibited tumor development in vivo. Four groups of single cells (MDA-MB-231, LV-NC, LV-CXCL16, and MCF-7) were injected subcutaneously into mice and tumor growth was monitored. (a) CXCL16 overexpression led to a decrease in tumorigenesis. *P < 0.05 compared with MCF-7. #P < 0.05 compared with MDA-MB-231. ▼P < 0.01 compared with MCF-7. (b) CXCL16 overexpression reduced tumor volume and weight. #P < 0.05 compared with MDA-MB-231. ▼P < 0.01 compared with MCF-7. *P < 0.05 compared with MCF7.
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fig4: CXCL16 expression inhibited tumor development in vivo. Four groups of single cells (MDA-MB-231, LV-NC, LV-CXCL16, and MCF-7) were injected subcutaneously into mice and tumor growth was monitored. (a) CXCL16 overexpression led to a decrease in tumorigenesis. *P < 0.05 compared with MCF-7. #P < 0.05 compared with MDA-MB-231. ▼P < 0.01 compared with MCF-7. (b) CXCL16 overexpression reduced tumor volume and weight. #P < 0.05 compared with MDA-MB-231. ▼P < 0.01 compared with MCF-7. *P < 0.05 compared with MCF7.

Mentions: As shown in Figure 4(a), CXCL16-overexpressing MDA-MB-231 showed delayed tumor progression as compared to MDA-MB-231 or the positive control (MCF-7) (P < 0.05). Tumor volumes and weights for CXCL16-overexpressing groups were accordingly found to be significantly reduced (P < 0.05) (Figure 4(b)).


Chemokine CXCL16 expression suppresses migration and invasiveness and induces apoptosis in breast cancer cells.

Fang Y, Henderson FC, Yi Q, Lei Q, Li Y, Chen N - Mediators Inflamm. (2014)

CXCL16 expression inhibited tumor development in vivo. Four groups of single cells (MDA-MB-231, LV-NC, LV-CXCL16, and MCF-7) were injected subcutaneously into mice and tumor growth was monitored. (a) CXCL16 overexpression led to a decrease in tumorigenesis. *P < 0.05 compared with MCF-7. #P < 0.05 compared with MDA-MB-231. ▼P < 0.01 compared with MCF-7. (b) CXCL16 overexpression reduced tumor volume and weight. #P < 0.05 compared with MDA-MB-231. ▼P < 0.01 compared with MCF-7. *P < 0.05 compared with MCF7.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016906&req=5

fig4: CXCL16 expression inhibited tumor development in vivo. Four groups of single cells (MDA-MB-231, LV-NC, LV-CXCL16, and MCF-7) were injected subcutaneously into mice and tumor growth was monitored. (a) CXCL16 overexpression led to a decrease in tumorigenesis. *P < 0.05 compared with MCF-7. #P < 0.05 compared with MDA-MB-231. ▼P < 0.01 compared with MCF-7. (b) CXCL16 overexpression reduced tumor volume and weight. #P < 0.05 compared with MDA-MB-231. ▼P < 0.01 compared with MCF-7. *P < 0.05 compared with MCF7.
Mentions: As shown in Figure 4(a), CXCL16-overexpressing MDA-MB-231 showed delayed tumor progression as compared to MDA-MB-231 or the positive control (MCF-7) (P < 0.05). Tumor volumes and weights for CXCL16-overexpressing groups were accordingly found to be significantly reduced (P < 0.05) (Figure 4(b)).

Bottom Line: Increasing evidence argues that soluble CXCL16 promotes proliferation, migration, and invasion of cancer cells in vitro.Overexpression of CXCL16 in MDA-MB-231 cells led to a decrease in cell invasion and migration and induced apoptosis of the cells; downregulation of CXCL16 in MCF-7 cells increased cell migration and invasiveness.Consistent with the in vitro data, CXCL16 overexpression inhibited tumorigenesis in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 37 Guoxuexiang, Chengdu, Sichuan 610041, China.

ABSTRACT

Background: Increasing evidence argues that soluble CXCL16 promotes proliferation, migration, and invasion of cancer cells in vitro. However, the role of transmembrane or cellular CXCL16 in cancer remains relatively unknown. In this study, we determine the function of cellular CXCL16 as tumor suppressor in breast cancer cells.

Methods: Expression of cellular CXCL16 in breast cancer cell lines was determined at both RNA and protein levels. In vitro and in vivo studies that overexpressed or downregulated CXCL16 were conducted in breast cancer cells.

Results: We report differential expression of cellular CXCL16 in breast cancer cell lines that was negatively correlated with cell invasiveness and migration. Overexpression of CXCL16 in MDA-MB-231 cells led to a decrease in cell invasion and migration and induced apoptosis of the cells; downregulation of CXCL16 in MCF-7 cells increased cell migration and invasiveness. Consistent with the in vitro data, CXCL16 overexpression inhibited tumorigenesis in vivo.

Conclusions: Cellular CXCL16 suppresses invasion and metastasis of breast cancer cells in vitro and inhibits tumorigenesis in vivo. Targeting of cellular CXCL16 expression is a potential therapeutic strategy for breast cancer.

Show MeSH
Related in: MedlinePlus