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TRIM29 as a novel biomarker in pancreatic adenocarcinoma.

Sun H, Dai X, Han B - Dis. Markers (2014)

Bottom Line: TRIM29 protein expression was significantly correlated with lymph node metastasis (P = 0.019).Patients with positive TRIM29 expression showed both shorter overall survival and shorter recurrence-free survival than those with negative TRIM29 expression.Our results indicate that TRIM29 promotes tumor progression and may be a novel prognostic marker for pancreatic ductal adenocarcinoma.

View Article: PubMed Central - PubMed

Affiliation: Department of Biliary and Vascular Surgery, Shengjing Hospital of China Medical University, Heping District, Shenyang 110004, China.

ABSTRACT

Background and aim: Tripartite motif-containing 29 (TRIM29) is structurally a member of the tripartite motif family of proteins and is involved in diverse human cancers. However, its role in pancreatic cancer remains unclear.

Methods: The expression pattern of TRIM29 in pancreatic ductal adenocarcinoma was assessed by immunocytochemistry. Multivariate logistic regression analysis was used to investigate the association between TRIM29 and clinical characteristics. In vitro analyses by scratch wound healing assay and invasion assays were performed using the pancreatic cancer cell lines.

Results: Immunohistochemical analysis showed TRIM29 expression in pancreatic cancer tissues was significantly higher  (n = 186) than that in matched adjacent nontumor tissues. TRIM29 protein expression was significantly correlated with lymph node metastasis (P = 0.019). Patients with positive TRIM29 expression showed both shorter overall survival and shorter recurrence-free survival than those with negative TRIM29 expression. Multivariate analysis revealed that TRIM29 was an independent factor for pancreatic cancer over survival (HR = 2.180, 95% CI: 1.324-4.198, P = 0.011). In vitro, TRIM29 knockdown resulted in inhibition of pancreatic cancer cell proliferation, migration, and invasion.

Conclusions: Our results indicate that TRIM29 promotes tumor progression and may be a novel prognostic marker for pancreatic ductal adenocarcinoma.

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Related in: MedlinePlus

Effect of TRIM29 knockdown on pancreatic cancer cell migration and invasion. (a) Cells transfected with scrambled siRNA (si-Scramble) or siRNA targeting TRIM29 (siTRIM29) for 48 h and after another 48 h, migrated cells were stained and counted under a microscope (×10). Representative images were shown. (b) Number of migrated cells shown (×10). Data was shown as mean ± SD from five fields. *P < 0.05 versus the si-Scramble group. 1: si-Scramble; 2: siTRIM29.
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fig4: Effect of TRIM29 knockdown on pancreatic cancer cell migration and invasion. (a) Cells transfected with scrambled siRNA (si-Scramble) or siRNA targeting TRIM29 (siTRIM29) for 48 h and after another 48 h, migrated cells were stained and counted under a microscope (×10). Representative images were shown. (b) Number of migrated cells shown (×10). Data was shown as mean ± SD from five fields. *P < 0.05 versus the si-Scramble group. 1: si-Scramble; 2: siTRIM29.

Mentions: In cell migration assay (Figure 4(a)), siTRIM29 treatment significantly reduced cell numbers translocating across the membranes by an average of more than 50% in both SW1990 cellsand BxPC3 cells, respectively, when comparedwith si-Scramble treatment after 24 h incubation. Similar trends were observed in cell invasion assay (Figure 4(b)). siTRIM29 treatment significantly reduced the invasion in SW1990 cells and BxPC3 cells by more than 50%. These results indicate that TRIM29 expression is correlated with cell proliferation, migration, and invasion in pancreatic cancer cells.


TRIM29 as a novel biomarker in pancreatic adenocarcinoma.

Sun H, Dai X, Han B - Dis. Markers (2014)

Effect of TRIM29 knockdown on pancreatic cancer cell migration and invasion. (a) Cells transfected with scrambled siRNA (si-Scramble) or siRNA targeting TRIM29 (siTRIM29) for 48 h and after another 48 h, migrated cells were stained and counted under a microscope (×10). Representative images were shown. (b) Number of migrated cells shown (×10). Data was shown as mean ± SD from five fields. *P < 0.05 versus the si-Scramble group. 1: si-Scramble; 2: siTRIM29.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016900&req=5

fig4: Effect of TRIM29 knockdown on pancreatic cancer cell migration and invasion. (a) Cells transfected with scrambled siRNA (si-Scramble) or siRNA targeting TRIM29 (siTRIM29) for 48 h and after another 48 h, migrated cells were stained and counted under a microscope (×10). Representative images were shown. (b) Number of migrated cells shown (×10). Data was shown as mean ± SD from five fields. *P < 0.05 versus the si-Scramble group. 1: si-Scramble; 2: siTRIM29.
Mentions: In cell migration assay (Figure 4(a)), siTRIM29 treatment significantly reduced cell numbers translocating across the membranes by an average of more than 50% in both SW1990 cellsand BxPC3 cells, respectively, when comparedwith si-Scramble treatment after 24 h incubation. Similar trends were observed in cell invasion assay (Figure 4(b)). siTRIM29 treatment significantly reduced the invasion in SW1990 cells and BxPC3 cells by more than 50%. These results indicate that TRIM29 expression is correlated with cell proliferation, migration, and invasion in pancreatic cancer cells.

Bottom Line: TRIM29 protein expression was significantly correlated with lymph node metastasis (P = 0.019).Patients with positive TRIM29 expression showed both shorter overall survival and shorter recurrence-free survival than those with negative TRIM29 expression.Our results indicate that TRIM29 promotes tumor progression and may be a novel prognostic marker for pancreatic ductal adenocarcinoma.

View Article: PubMed Central - PubMed

Affiliation: Department of Biliary and Vascular Surgery, Shengjing Hospital of China Medical University, Heping District, Shenyang 110004, China.

ABSTRACT

Background and aim: Tripartite motif-containing 29 (TRIM29) is structurally a member of the tripartite motif family of proteins and is involved in diverse human cancers. However, its role in pancreatic cancer remains unclear.

Methods: The expression pattern of TRIM29 in pancreatic ductal adenocarcinoma was assessed by immunocytochemistry. Multivariate logistic regression analysis was used to investigate the association between TRIM29 and clinical characteristics. In vitro analyses by scratch wound healing assay and invasion assays were performed using the pancreatic cancer cell lines.

Results: Immunohistochemical analysis showed TRIM29 expression in pancreatic cancer tissues was significantly higher  (n = 186) than that in matched adjacent nontumor tissues. TRIM29 protein expression was significantly correlated with lymph node metastasis (P = 0.019). Patients with positive TRIM29 expression showed both shorter overall survival and shorter recurrence-free survival than those with negative TRIM29 expression. Multivariate analysis revealed that TRIM29 was an independent factor for pancreatic cancer over survival (HR = 2.180, 95% CI: 1.324-4.198, P = 0.011). In vitro, TRIM29 knockdown resulted in inhibition of pancreatic cancer cell proliferation, migration, and invasion.

Conclusions: Our results indicate that TRIM29 promotes tumor progression and may be a novel prognostic marker for pancreatic ductal adenocarcinoma.

Show MeSH
Related in: MedlinePlus