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Upregulated PD-1 Expression Is Associated with the Development of Systemic Lupus Erythematosus, but Not the PD-1.1 Allele of the PDCD1 Gene.

Jiao Q, Liu C, Yang Z, Ding Q, Wang M, Li M, Zhu T, Qian H, Li W, Tu N, Fang F, Ye L, Zhao Z, Qian Q - Int J Genomics (2014)

Bottom Line: The PD-1 expression levels of SLE patients were significantly increased compared with those of the healthy controls.The upregulated PD-1 expression levels in SLE patients were greatly associated with SLEDAI scores.No significant difference was found between PD-1 expression levels and SNP rs36084323.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, China.

ABSTRACT
Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease with complicated genetic inheritance. Programmed death 1 (PD-1), a negative T cell regulator to maintain peripheral tolerance, induces negative signals to T cells during interaction with its ligands and is therefore a candidate gene in the development of SLE. In order to examine whether expression levels of PD-1 contribute to the pathogenesis of SLE, 30 patients with SLE and 30 controls were recruited and their PD-1 expression levels in peripheral blood mononuclear cells (PBMCs) were measured via flow cytometry and quantitative real-time-reverse transcription polymerase chain reaction (RT-PCR). Also, whether PD-1 expression levels are associated with the variant of the SNP rs36084323 and the SLE Disease Activity Index (SLEDAI) was studied in this work. The PD-1 expression levels of SLE patients were significantly increased compared with those of the healthy controls. The upregulated PD-1 expression levels in SLE patients were greatly associated with SLEDAI scores. No significant difference was found between PD-1 expression levels and SNP rs36084323. The results suggest that increased expression of PD-1 may correlate with the pathogenesis of SLE, upregulated PD-1 expression may be a biomarker for SLE diagnosis, and PD-1 inhibitor may be useful to SLE treatment.

No MeSH data available.


Related in: MedlinePlus

Correlation of upregulated PD-1 expression levels with SLEDAI in PBMCs. The association of SLEDAI with upregulated PD-1 expression on CD4+ T cell (a), CD8+ T cell (b), CD56+ T cell (c), and mRNA expression of PD-1 in PBMCs (d).
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fig2: Correlation of upregulated PD-1 expression levels with SLEDAI in PBMCs. The association of SLEDAI with upregulated PD-1 expression on CD4+ T cell (a), CD8+ T cell (b), CD56+ T cell (c), and mRNA expression of PD-1 in PBMCs (d).

Mentions: In order to determine whether upregulated PD-1 expression is related to SLEDAI, correlation analysis was carried out. The results have shown that SLEDAI scores were significantly related to upregulated PD-1 expression on CD4+ T cells, CD8+ T cells, CD56+ T cells, and increased PD-1 mRNA expression levels in PBMCs from PB samples of SLE patients (Figure 2), which indicates that upregulated PD-1 expression may be involved in the pathogenesis of SLE.


Upregulated PD-1 Expression Is Associated with the Development of Systemic Lupus Erythematosus, but Not the PD-1.1 Allele of the PDCD1 Gene.

Jiao Q, Liu C, Yang Z, Ding Q, Wang M, Li M, Zhu T, Qian H, Li W, Tu N, Fang F, Ye L, Zhao Z, Qian Q - Int J Genomics (2014)

Correlation of upregulated PD-1 expression levels with SLEDAI in PBMCs. The association of SLEDAI with upregulated PD-1 expression on CD4+ T cell (a), CD8+ T cell (b), CD56+ T cell (c), and mRNA expression of PD-1 in PBMCs (d).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016872&req=5

fig2: Correlation of upregulated PD-1 expression levels with SLEDAI in PBMCs. The association of SLEDAI with upregulated PD-1 expression on CD4+ T cell (a), CD8+ T cell (b), CD56+ T cell (c), and mRNA expression of PD-1 in PBMCs (d).
Mentions: In order to determine whether upregulated PD-1 expression is related to SLEDAI, correlation analysis was carried out. The results have shown that SLEDAI scores were significantly related to upregulated PD-1 expression on CD4+ T cells, CD8+ T cells, CD56+ T cells, and increased PD-1 mRNA expression levels in PBMCs from PB samples of SLE patients (Figure 2), which indicates that upregulated PD-1 expression may be involved in the pathogenesis of SLE.

Bottom Line: The PD-1 expression levels of SLE patients were significantly increased compared with those of the healthy controls.The upregulated PD-1 expression levels in SLE patients were greatly associated with SLEDAI scores.No significant difference was found between PD-1 expression levels and SNP rs36084323.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, China.

ABSTRACT
Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease with complicated genetic inheritance. Programmed death 1 (PD-1), a negative T cell regulator to maintain peripheral tolerance, induces negative signals to T cells during interaction with its ligands and is therefore a candidate gene in the development of SLE. In order to examine whether expression levels of PD-1 contribute to the pathogenesis of SLE, 30 patients with SLE and 30 controls were recruited and their PD-1 expression levels in peripheral blood mononuclear cells (PBMCs) were measured via flow cytometry and quantitative real-time-reverse transcription polymerase chain reaction (RT-PCR). Also, whether PD-1 expression levels are associated with the variant of the SNP rs36084323 and the SLE Disease Activity Index (SLEDAI) was studied in this work. The PD-1 expression levels of SLE patients were significantly increased compared with those of the healthy controls. The upregulated PD-1 expression levels in SLE patients were greatly associated with SLEDAI scores. No significant difference was found between PD-1 expression levels and SNP rs36084323. The results suggest that increased expression of PD-1 may correlate with the pathogenesis of SLE, upregulated PD-1 expression may be a biomarker for SLE diagnosis, and PD-1 inhibitor may be useful to SLE treatment.

No MeSH data available.


Related in: MedlinePlus