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Biocontrol Activity of the Local Strain of Metschnikowia pulcherrima on Different Postharvest Pathogens.

Türkel S, Korukluoğlu M, Yavuz M - Biotechnol Res Int (2014)

Bottom Line: The strains of the yeast Metschnikowia pulcherrima have strong biocontrol activity against various microorganisms.Biocontrol activity of M. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin.M. pulcherrima UMY15 was a very effective biocontrol agent against Penicillium roqueforti, P. italicum, P. expansum, and Aspergillus oryzae in in-vitro plate tests.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey.

ABSTRACT
The strains of the yeast Metschnikowia pulcherrima have strong biocontrol activity against various microorganisms. Biocontrol activity of M. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin. Biocontrol activity of pulcherrimin producing strain, M. pulcherrima UMY15, isolated from local vineyards, was tested on different molds that cause food spoilage. M. pulcherrima UMY15 was a very effective biocontrol agent against Penicillium roqueforti, P. italicum, P. expansum, and Aspergillus oryzae in in-vitro plate tests. However, the inhibitory activity of M. pulcherrima UMY15 was less effective on Fusarium sp. and A. niger species in biocontrol assays. In addition, M. pulcherrima UMY15 strain completely inhibited the germination and mycelia growth of A. oryzae, A. parasiticus, and Fusarium sp. spores on artificial wounds of apples when they coinoculated with M. pulcherrima UMY15. Moreover, when coinoculated, M. pulcherrima UMY15 strain also inhibited the growth of P. roqueforti, P. italicum, P. expansum, A. oryzae, Fusarium sp., and Rhizopus sp. in grape juice, indicating that M. pulcherrima UMY15 can be used as a very effective biocontrol yeast against various species of postharvest pathogens, including Penicillium, Aspergillus, Fusarium, and Rhizopus.

No MeSH data available.


Related in: MedlinePlus

Examples of the inhibitory effects of M. pulcherrima UMY15 strain on different fungal species on artificially wound apples. Infection assays for Fusarium sp. (a), A. Oryzae (b), A. niger (c), and A. parasiticus (d) were done on artificial wounds on apple slices. Inoculations on artificial wounds are 1: mold spores only, 2: M. pulcherrima only, and 3: mold spores and M. pulcherrima inoculations.
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fig2: Examples of the inhibitory effects of M. pulcherrima UMY15 strain on different fungal species on artificially wound apples. Infection assays for Fusarium sp. (a), A. Oryzae (b), A. niger (c), and A. parasiticus (d) were done on artificial wounds on apple slices. Inoculations on artificial wounds are 1: mold spores only, 2: M. pulcherrima only, and 3: mold spores and M. pulcherrima inoculations.

Mentions: Coexistence or cocultivation of M. pulcherrima strain UMY15 with Fusarium sp. spores completely inhibited the germination of Fusarium sp. spores and spoilage of artificially wound apples (Table 2, Figure 2(a)). In the absence of M. pulcherrima strain, Fusarium sp. spores germinated and mycelia growth led to the formation of 12 mm spoilage zones on apple wounds. However, when M. pulcherrima strain and Fusarium spores coinoculated on same wound, there were no spoilage zones on artificial wounds on apples (Table 2, Figure 2(a)). Biocontrol activity of M. pulcherrima on Fusarium was determined as 100%.


Biocontrol Activity of the Local Strain of Metschnikowia pulcherrima on Different Postharvest Pathogens.

Türkel S, Korukluoğlu M, Yavuz M - Biotechnol Res Int (2014)

Examples of the inhibitory effects of M. pulcherrima UMY15 strain on different fungal species on artificially wound apples. Infection assays for Fusarium sp. (a), A. Oryzae (b), A. niger (c), and A. parasiticus (d) were done on artificial wounds on apple slices. Inoculations on artificial wounds are 1: mold spores only, 2: M. pulcherrima only, and 3: mold spores and M. pulcherrima inoculations.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016867&req=5

fig2: Examples of the inhibitory effects of M. pulcherrima UMY15 strain on different fungal species on artificially wound apples. Infection assays for Fusarium sp. (a), A. Oryzae (b), A. niger (c), and A. parasiticus (d) were done on artificial wounds on apple slices. Inoculations on artificial wounds are 1: mold spores only, 2: M. pulcherrima only, and 3: mold spores and M. pulcherrima inoculations.
Mentions: Coexistence or cocultivation of M. pulcherrima strain UMY15 with Fusarium sp. spores completely inhibited the germination of Fusarium sp. spores and spoilage of artificially wound apples (Table 2, Figure 2(a)). In the absence of M. pulcherrima strain, Fusarium sp. spores germinated and mycelia growth led to the formation of 12 mm spoilage zones on apple wounds. However, when M. pulcherrima strain and Fusarium spores coinoculated on same wound, there were no spoilage zones on artificial wounds on apples (Table 2, Figure 2(a)). Biocontrol activity of M. pulcherrima on Fusarium was determined as 100%.

Bottom Line: The strains of the yeast Metschnikowia pulcherrima have strong biocontrol activity against various microorganisms.Biocontrol activity of M. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin.M. pulcherrima UMY15 was a very effective biocontrol agent against Penicillium roqueforti, P. italicum, P. expansum, and Aspergillus oryzae in in-vitro plate tests.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey.

ABSTRACT
The strains of the yeast Metschnikowia pulcherrima have strong biocontrol activity against various microorganisms. Biocontrol activity of M. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin. Biocontrol activity of pulcherrimin producing strain, M. pulcherrima UMY15, isolated from local vineyards, was tested on different molds that cause food spoilage. M. pulcherrima UMY15 was a very effective biocontrol agent against Penicillium roqueforti, P. italicum, P. expansum, and Aspergillus oryzae in in-vitro plate tests. However, the inhibitory activity of M. pulcherrima UMY15 was less effective on Fusarium sp. and A. niger species in biocontrol assays. In addition, M. pulcherrima UMY15 strain completely inhibited the germination and mycelia growth of A. oryzae, A. parasiticus, and Fusarium sp. spores on artificial wounds of apples when they coinoculated with M. pulcherrima UMY15. Moreover, when coinoculated, M. pulcherrima UMY15 strain also inhibited the growth of P. roqueforti, P. italicum, P. expansum, A. oryzae, Fusarium sp., and Rhizopus sp. in grape juice, indicating that M. pulcherrima UMY15 can be used as a very effective biocontrol yeast against various species of postharvest pathogens, including Penicillium, Aspergillus, Fusarium, and Rhizopus.

No MeSH data available.


Related in: MedlinePlus