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Biocontrol Activity of the Local Strain of Metschnikowia pulcherrima on Different Postharvest Pathogens.

Türkel S, Korukluoğlu M, Yavuz M - Biotechnol Res Int (2014)

Bottom Line: The strains of the yeast Metschnikowia pulcherrima have strong biocontrol activity against various microorganisms.Biocontrol activity of M. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin.M. pulcherrima UMY15 was a very effective biocontrol agent against Penicillium roqueforti, P. italicum, P. expansum, and Aspergillus oryzae in in-vitro plate tests.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey.

ABSTRACT
The strains of the yeast Metschnikowia pulcherrima have strong biocontrol activity against various microorganisms. Biocontrol activity of M. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin. Biocontrol activity of pulcherrimin producing strain, M. pulcherrima UMY15, isolated from local vineyards, was tested on different molds that cause food spoilage. M. pulcherrima UMY15 was a very effective biocontrol agent against Penicillium roqueforti, P. italicum, P. expansum, and Aspergillus oryzae in in-vitro plate tests. However, the inhibitory activity of M. pulcherrima UMY15 was less effective on Fusarium sp. and A. niger species in biocontrol assays. In addition, M. pulcherrima UMY15 strain completely inhibited the germination and mycelia growth of A. oryzae, A. parasiticus, and Fusarium sp. spores on artificial wounds of apples when they coinoculated with M. pulcherrima UMY15. Moreover, when coinoculated, M. pulcherrima UMY15 strain also inhibited the growth of P. roqueforti, P. italicum, P. expansum, A. oryzae, Fusarium sp., and Rhizopus sp. in grape juice, indicating that M. pulcherrima UMY15 can be used as a very effective biocontrol yeast against various species of postharvest pathogens, including Penicillium, Aspergillus, Fusarium, and Rhizopus.

No MeSH data available.


Related in: MedlinePlus

M. pulcherrima inhibits the growth of different molds on SD plates. Lawns on plate (a) P. expansum, plate (b) P. roqueforti, plate (c) A. oryzae, and plate (d) A. parasiticus.
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fig1: M. pulcherrima inhibits the growth of different molds on SD plates. Lawns on plate (a) P. expansum, plate (b) P. roqueforti, plate (c) A. oryzae, and plate (d) A. parasiticus.

Mentions: The inhibitory or antagonistic effects of M. pulcherrima UMY15 strain on the spore germination and the growth of eight different mold species were first investigated by plate tests. M. pulcherrima UMY15 strain's suspensions were applied onto the spores of molds used in this study as described. Germinations and hyphal growth of P. roqueforti, P. italicum, and P. expansum spores were significantly inhibited by M. pulcherrima UMY15 strain on SD petri plates (Table 1 and Figures 1(a) and 1(b)). At the end of 24 hours incubation period, 3-4 mm of inhibition zones were clearly visible on the spore germination plates of Penicillium species used in this study. However, unlike Penicillium species, germination of Fusarium sp., Rhizopus sp., and A. niger spores was inhibited at low levels by M. pulcherrima strain. Inhibition zones on the spore germination plates of these three fungal strains were approximately 1 mm (Table 1). Nonetheless, there is a significant level of inhibitory effect of M. pulcherrima strain on two other Aspergillus species, A. oryzae and A. parasiticus, respectively (Table 1 and Figures 1(c) and 1(d)). Inhibition zones on the spore germination plates of A. oryzae and A. parasiticus were 2-3 mm after 24 hours of incubation period.


Biocontrol Activity of the Local Strain of Metschnikowia pulcherrima on Different Postharvest Pathogens.

Türkel S, Korukluoğlu M, Yavuz M - Biotechnol Res Int (2014)

M. pulcherrima inhibits the growth of different molds on SD plates. Lawns on plate (a) P. expansum, plate (b) P. roqueforti, plate (c) A. oryzae, and plate (d) A. parasiticus.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016867&req=5

fig1: M. pulcherrima inhibits the growth of different molds on SD plates. Lawns on plate (a) P. expansum, plate (b) P. roqueforti, plate (c) A. oryzae, and plate (d) A. parasiticus.
Mentions: The inhibitory or antagonistic effects of M. pulcherrima UMY15 strain on the spore germination and the growth of eight different mold species were first investigated by plate tests. M. pulcherrima UMY15 strain's suspensions were applied onto the spores of molds used in this study as described. Germinations and hyphal growth of P. roqueforti, P. italicum, and P. expansum spores were significantly inhibited by M. pulcherrima UMY15 strain on SD petri plates (Table 1 and Figures 1(a) and 1(b)). At the end of 24 hours incubation period, 3-4 mm of inhibition zones were clearly visible on the spore germination plates of Penicillium species used in this study. However, unlike Penicillium species, germination of Fusarium sp., Rhizopus sp., and A. niger spores was inhibited at low levels by M. pulcherrima strain. Inhibition zones on the spore germination plates of these three fungal strains were approximately 1 mm (Table 1). Nonetheless, there is a significant level of inhibitory effect of M. pulcherrima strain on two other Aspergillus species, A. oryzae and A. parasiticus, respectively (Table 1 and Figures 1(c) and 1(d)). Inhibition zones on the spore germination plates of A. oryzae and A. parasiticus were 2-3 mm after 24 hours of incubation period.

Bottom Line: The strains of the yeast Metschnikowia pulcherrima have strong biocontrol activity against various microorganisms.Biocontrol activity of M. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin.M. pulcherrima UMY15 was a very effective biocontrol agent against Penicillium roqueforti, P. italicum, P. expansum, and Aspergillus oryzae in in-vitro plate tests.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey.

ABSTRACT
The strains of the yeast Metschnikowia pulcherrima have strong biocontrol activity against various microorganisms. Biocontrol activity of M. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin. Biocontrol activity of pulcherrimin producing strain, M. pulcherrima UMY15, isolated from local vineyards, was tested on different molds that cause food spoilage. M. pulcherrima UMY15 was a very effective biocontrol agent against Penicillium roqueforti, P. italicum, P. expansum, and Aspergillus oryzae in in-vitro plate tests. However, the inhibitory activity of M. pulcherrima UMY15 was less effective on Fusarium sp. and A. niger species in biocontrol assays. In addition, M. pulcherrima UMY15 strain completely inhibited the germination and mycelia growth of A. oryzae, A. parasiticus, and Fusarium sp. spores on artificial wounds of apples when they coinoculated with M. pulcherrima UMY15. Moreover, when coinoculated, M. pulcherrima UMY15 strain also inhibited the growth of P. roqueforti, P. italicum, P. expansum, A. oryzae, Fusarium sp., and Rhizopus sp. in grape juice, indicating that M. pulcherrima UMY15 can be used as a very effective biocontrol yeast against various species of postharvest pathogens, including Penicillium, Aspergillus, Fusarium, and Rhizopus.

No MeSH data available.


Related in: MedlinePlus