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A Nonhost Peptidase Inhibitor of ~14 kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera.

Pandey PK, Singh D, Singh S, Khan MY, Jamal F - Biochem Res Int (2014)

Bottom Line: B. monosperma peptidase inhibitor on 12% denaturing polyacrylamide gel electrophoresis exhibited a single protein band of ~14 kDa with or without reduction.B. monosperma peptidase inhibitor dose for 50% mortality and weight reduction by 50% were 0.5% w/w and 0.10% w/w, respectively.Supplementing B. monosperma peptidase inhibitor in artificial diet at 0.1% w/w, both the efficiencies of conversion of ingested as well as digested food were downregulated, whereas approximate digestibility and metabolic cost were enhanced.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry [DST-FIST & UGC-SAP Supported], Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.

ABSTRACT
Helicoverpa armigera is one of the major devastating pests of crop plants. In this context a serine peptidase inhibitor purified from the seeds of Butea monosperma was evaluated for its effect on developmental physiology of H. armigera larvae. B. monosperma peptidase inhibitor on 12% denaturing polyacrylamide gel electrophoresis exhibited a single protein band of ~14 kDa with or without reduction. In vitro studies towards total gut proteolytic enzymes of H. armigera and bovine trypsin indicated measurable inhibitory activity. B. monosperma peptidase inhibitor dose for 50% mortality and weight reduction by 50% were 0.5% w/w and 0.10% w/w, respectively. The IC50 of B. monosperma peptidase inhibitor against total H. armigera gut proteinases activity was 2.0 µg/mL. The larval feeding assays suggested B. monosperma peptidase inhibitor to be toxic as reflected by its retarded growth and development, consequently affecting fertility and fecundity of pest and prolonging the larval-pupal duration of the insect life cycle of H. armigera. Supplementing B. monosperma peptidase inhibitor in artificial diet at 0.1% w/w, both the efficiencies of conversion of ingested as well as digested food were downregulated, whereas approximate digestibility and metabolic cost were enhanced. The efficacy of Butea monosperma peptidase inhibitor against progressive growth and development of H. armigera suggest its usefulness in insect pest management of food crops.

No MeSH data available.


Related in: MedlinePlus

Effect of BmPI on H. armigera larval and pupal development. Number of larvae taken for study at each concentration (n = 52). d: days, n: number of larvae.
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fig4: Effect of BmPI on H. armigera larval and pupal development. Number of larvae taken for study at each concentration (n = 52). d: days, n: number of larvae.

Mentions: Antifeedants could be used to protect crops until slow acting biopesticides produce their effect [34]. The isolated proteinase inhibitor in this study clearly showed high antifeedant activity against H. armigera suggesting its greater susceptibility. Kuhar et al. (2013) had also reported antifeedant activity of a double headed peptidase inhibitor from Dolichos biflorus seeds [35]. In addition, BmPI (0.5% w/w) showed strong larvicidal and pupicidal activity of 50% and 92.6%, respectively (Figure 4).


A Nonhost Peptidase Inhibitor of ~14 kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera.

Pandey PK, Singh D, Singh S, Khan MY, Jamal F - Biochem Res Int (2014)

Effect of BmPI on H. armigera larval and pupal development. Number of larvae taken for study at each concentration (n = 52). d: days, n: number of larvae.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016866&req=5

fig4: Effect of BmPI on H. armigera larval and pupal development. Number of larvae taken for study at each concentration (n = 52). d: days, n: number of larvae.
Mentions: Antifeedants could be used to protect crops until slow acting biopesticides produce their effect [34]. The isolated proteinase inhibitor in this study clearly showed high antifeedant activity against H. armigera suggesting its greater susceptibility. Kuhar et al. (2013) had also reported antifeedant activity of a double headed peptidase inhibitor from Dolichos biflorus seeds [35]. In addition, BmPI (0.5% w/w) showed strong larvicidal and pupicidal activity of 50% and 92.6%, respectively (Figure 4).

Bottom Line: B. monosperma peptidase inhibitor on 12% denaturing polyacrylamide gel electrophoresis exhibited a single protein band of ~14 kDa with or without reduction.B. monosperma peptidase inhibitor dose for 50% mortality and weight reduction by 50% were 0.5% w/w and 0.10% w/w, respectively.Supplementing B. monosperma peptidase inhibitor in artificial diet at 0.1% w/w, both the efficiencies of conversion of ingested as well as digested food were downregulated, whereas approximate digestibility and metabolic cost were enhanced.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry [DST-FIST & UGC-SAP Supported], Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.

ABSTRACT
Helicoverpa armigera is one of the major devastating pests of crop plants. In this context a serine peptidase inhibitor purified from the seeds of Butea monosperma was evaluated for its effect on developmental physiology of H. armigera larvae. B. monosperma peptidase inhibitor on 12% denaturing polyacrylamide gel electrophoresis exhibited a single protein band of ~14 kDa with or without reduction. In vitro studies towards total gut proteolytic enzymes of H. armigera and bovine trypsin indicated measurable inhibitory activity. B. monosperma peptidase inhibitor dose for 50% mortality and weight reduction by 50% were 0.5% w/w and 0.10% w/w, respectively. The IC50 of B. monosperma peptidase inhibitor against total H. armigera gut proteinases activity was 2.0 µg/mL. The larval feeding assays suggested B. monosperma peptidase inhibitor to be toxic as reflected by its retarded growth and development, consequently affecting fertility and fecundity of pest and prolonging the larval-pupal duration of the insect life cycle of H. armigera. Supplementing B. monosperma peptidase inhibitor in artificial diet at 0.1% w/w, both the efficiencies of conversion of ingested as well as digested food were downregulated, whereas approximate digestibility and metabolic cost were enhanced. The efficacy of Butea monosperma peptidase inhibitor against progressive growth and development of H. armigera suggest its usefulness in insect pest management of food crops.

No MeSH data available.


Related in: MedlinePlus