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Preferential autoimmune response in prostate cancer to cyclin B1 in a panel of tumor-associated antigens.

Dai L, Li J, Ortega R, Qian W, Casiano CA, Zhang JY - J Immunol Res (2014)

Bottom Line: Autoantibodies to cyclin B1 and fourteen other TAAs were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting in 464 sera from patients with PCa, benign prostatic hyperplasia (BPH), and other controls.In the further analysis, 31.4% of sera from PCa patients at the early stage contained anti-cyclin B1 autoantibody, and even 29.4% of patients who had normal prostate-specific antigen (PSA) levels in their serum samples were observed anti-cyclin B1 positive.This study further supports the hypothesis that a customized TAA array can be used for enhancing anti-TAA autoantibody detection, and it may constitute a promising and powerful tool for immunodiagnosis of PCa.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USA.

ABSTRACT
Previous studies have demonstrated that sera from patients with prostate cancer (PCa) contain autoantibodies that react with tumor-associated antigens (TAAs). Autoantibodies to cyclin B1 and fourteen other TAAs were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting in 464 sera from patients with PCa, benign prostatic hyperplasia (BPH), and other controls. Autoantibodies to cyclin B1 were detected in 31.0% of sera from randomly selected patients with PCa versus 4.8% in sera with BPH. In the further analysis, 31.4% of sera from PCa patients at the early stage contained anti-cyclin B1 autoantibody, and even 29.4% of patients who had normal prostate-specific antigen (PSA) levels in their serum samples were observed anti-cyclin B1 positive. The cumulative positive rate of autoantibodies against seven selected TAAs (cyclin B1, survivin, p53, DFS70/LEDGFp75, RalA, MDM2, and NPM1) in PCa reached 80.5%, significantly higher than that in normal control sera. In summary, autoantibody to cyclin B1 might be a potential biomarker for the immunodiagnosis of early stage PCa, especially useful in patients with normal PSA level. This study further supports the hypothesis that a customized TAA array can be used for enhancing anti-TAA autoantibody detection, and it may constitute a promising and powerful tool for immunodiagnosis of PCa.

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Related in: MedlinePlus

Percentage of autoantibody responses to a panel of 15 TAAs. The percentages refer to antibody titers exceeding the mean + 3SD of normal human sera from ELISA. In this panel of 15 TAAs, the highest reactivity antigen was cyclin B1.
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fig3: Percentage of autoantibody responses to a panel of 15 TAAs. The percentages refer to antibody titers exceeding the mean + 3SD of normal human sera from ELISA. In this panel of 15 TAAs, the highest reactivity antigen was cyclin B1.

Mentions: Table 1 and Figure 3 show that PCa also contained autoantibodies to 14 other TAAs in addition to cyclin B1. Table 1 shows the frequency of autoantibodies to the panel of 15 TAAs using ELISA. Higher frequency of antibodies against individual TAA in PCa was found with TAAs such as survivin, p53, RalA, DFS70/LEDGFp75, MDM2, and NPM1 compared to normal human sera (P < 0.01) besides cyclin B1. Notably, in BPH, antibody frequency to any individual TAA ranged from 0 to 9.5% and there was no significant difference with NHS. The reactivity of anti-TAAs antibodies in normal human sera was very low, ranging from 0 to 3.4% to any individual TAA. Sera from patients with systemic rheumatic autoimmune disorders (SLE and PSS) also showed very low frequency of 15 anti-TAAs with no more than 6% and established that antibody reactivities observed were cancer specific and not attributable to nonspecific immunoreactivity.


Preferential autoimmune response in prostate cancer to cyclin B1 in a panel of tumor-associated antigens.

Dai L, Li J, Ortega R, Qian W, Casiano CA, Zhang JY - J Immunol Res (2014)

Percentage of autoantibody responses to a panel of 15 TAAs. The percentages refer to antibody titers exceeding the mean + 3SD of normal human sera from ELISA. In this panel of 15 TAAs, the highest reactivity antigen was cyclin B1.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016862&req=5

fig3: Percentage of autoantibody responses to a panel of 15 TAAs. The percentages refer to antibody titers exceeding the mean + 3SD of normal human sera from ELISA. In this panel of 15 TAAs, the highest reactivity antigen was cyclin B1.
Mentions: Table 1 and Figure 3 show that PCa also contained autoantibodies to 14 other TAAs in addition to cyclin B1. Table 1 shows the frequency of autoantibodies to the panel of 15 TAAs using ELISA. Higher frequency of antibodies against individual TAA in PCa was found with TAAs such as survivin, p53, RalA, DFS70/LEDGFp75, MDM2, and NPM1 compared to normal human sera (P < 0.01) besides cyclin B1. Notably, in BPH, antibody frequency to any individual TAA ranged from 0 to 9.5% and there was no significant difference with NHS. The reactivity of anti-TAAs antibodies in normal human sera was very low, ranging from 0 to 3.4% to any individual TAA. Sera from patients with systemic rheumatic autoimmune disorders (SLE and PSS) also showed very low frequency of 15 anti-TAAs with no more than 6% and established that antibody reactivities observed were cancer specific and not attributable to nonspecific immunoreactivity.

Bottom Line: Autoantibodies to cyclin B1 and fourteen other TAAs were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting in 464 sera from patients with PCa, benign prostatic hyperplasia (BPH), and other controls.In the further analysis, 31.4% of sera from PCa patients at the early stage contained anti-cyclin B1 autoantibody, and even 29.4% of patients who had normal prostate-specific antigen (PSA) levels in their serum samples were observed anti-cyclin B1 positive.This study further supports the hypothesis that a customized TAA array can be used for enhancing anti-TAA autoantibody detection, and it may constitute a promising and powerful tool for immunodiagnosis of PCa.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USA.

ABSTRACT
Previous studies have demonstrated that sera from patients with prostate cancer (PCa) contain autoantibodies that react with tumor-associated antigens (TAAs). Autoantibodies to cyclin B1 and fourteen other TAAs were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting in 464 sera from patients with PCa, benign prostatic hyperplasia (BPH), and other controls. Autoantibodies to cyclin B1 were detected in 31.0% of sera from randomly selected patients with PCa versus 4.8% in sera with BPH. In the further analysis, 31.4% of sera from PCa patients at the early stage contained anti-cyclin B1 autoantibody, and even 29.4% of patients who had normal prostate-specific antigen (PSA) levels in their serum samples were observed anti-cyclin B1 positive. The cumulative positive rate of autoantibodies against seven selected TAAs (cyclin B1, survivin, p53, DFS70/LEDGFp75, RalA, MDM2, and NPM1) in PCa reached 80.5%, significantly higher than that in normal control sera. In summary, autoantibody to cyclin B1 might be a potential biomarker for the immunodiagnosis of early stage PCa, especially useful in patients with normal PSA level. This study further supports the hypothesis that a customized TAA array can be used for enhancing anti-TAA autoantibody detection, and it may constitute a promising and powerful tool for immunodiagnosis of PCa.

Show MeSH
Related in: MedlinePlus