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Preferential autoimmune response in prostate cancer to cyclin B1 in a panel of tumor-associated antigens.

Dai L, Li J, Ortega R, Qian W, Casiano CA, Zhang JY - J Immunol Res (2014)

Bottom Line: Autoantibodies to cyclin B1 and fourteen other TAAs were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting in 464 sera from patients with PCa, benign prostatic hyperplasia (BPH), and other controls.In the further analysis, 31.4% of sera from PCa patients at the early stage contained anti-cyclin B1 autoantibody, and even 29.4% of patients who had normal prostate-specific antigen (PSA) levels in their serum samples were observed anti-cyclin B1 positive.This study further supports the hypothesis that a customized TAA array can be used for enhancing anti-TAA autoantibody detection, and it may constitute a promising and powerful tool for immunodiagnosis of PCa.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USA.

ABSTRACT
Previous studies have demonstrated that sera from patients with prostate cancer (PCa) contain autoantibodies that react with tumor-associated antigens (TAAs). Autoantibodies to cyclin B1 and fourteen other TAAs were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting in 464 sera from patients with PCa, benign prostatic hyperplasia (BPH), and other controls. Autoantibodies to cyclin B1 were detected in 31.0% of sera from randomly selected patients with PCa versus 4.8% in sera with BPH. In the further analysis, 31.4% of sera from PCa patients at the early stage contained anti-cyclin B1 autoantibody, and even 29.4% of patients who had normal prostate-specific antigen (PSA) levels in their serum samples were observed anti-cyclin B1 positive. The cumulative positive rate of autoantibodies against seven selected TAAs (cyclin B1, survivin, p53, DFS70/LEDGFp75, RalA, MDM2, and NPM1) in PCa reached 80.5%, significantly higher than that in normal control sera. In summary, autoantibody to cyclin B1 might be a potential biomarker for the immunodiagnosis of early stage PCa, especially useful in patients with normal PSA level. This study further supports the hypothesis that a customized TAA array can be used for enhancing anti-TAA autoantibody detection, and it may constitute a promising and powerful tool for immunodiagnosis of PCa.

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Related in: MedlinePlus

(a) Distribution of cyclin B1 antibody responses in different time points before and after surgery treatment. Anti-cyclin B1 antibody levels (OD values) in the 55 samples from 18 PCa patients were shown. Nineteen serum samples that are positive for anti-cyclin B1 are shown as filled black dots. The other samples are shown as unfilled circles. (b) Western blotting result of serial sera from a representative patient. Lane 1 is the sample before surgery and lanes 2 and 3 are ones after surgery.
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fig2: (a) Distribution of cyclin B1 antibody responses in different time points before and after surgery treatment. Anti-cyclin B1 antibody levels (OD values) in the 55 samples from 18 PCa patients were shown. Nineteen serum samples that are positive for anti-cyclin B1 are shown as filled black dots. The other samples are shown as unfilled circles. (b) Western blotting result of serial sera from a representative patient. Lane 1 is the sample before surgery and lanes 2 and 3 are ones after surgery.

Mentions: Fifty-five serial serum samples from 18 PCa patients were also detected for the expression of autoantibodies against cyclin B1. Serum samples from all these patients had been collected after the diagnosis of PCa and serum samples were also collected every three months after they had been taken surgical resection. At least two to four samples after surgery were available for each patient. These are very valuable serial serum samples from the patients with surgery and the detection of autoantibodies of these samples might reflect the prognosis after surgery. As shown in Figure 2(a), only six serum samples from 18 PCa patients before surgery reacted over the cut-off OD value of cyclin B1 autoantibody. However, 13 blood samples collected after surgery showed positive result in ELISA with higher OD value. There is likely association between cyclin B1 antibody level and the time before diagnosis. The closer to the time of diagnosis, the higher autoantibody level performed. All of the 55 serum samples were also analyzed by Western blotting to confirm the results from ELISA. Figure 2(b) shows a representative patient, who had no anti-cyclin B1 antibody before surgery (02/12/2003); however, the antibody weakly occurred six months after surgery (08/28/2003) and showed increased reactivity one year after surgery (01/22/2004).


Preferential autoimmune response in prostate cancer to cyclin B1 in a panel of tumor-associated antigens.

Dai L, Li J, Ortega R, Qian W, Casiano CA, Zhang JY - J Immunol Res (2014)

(a) Distribution of cyclin B1 antibody responses in different time points before and after surgery treatment. Anti-cyclin B1 antibody levels (OD values) in the 55 samples from 18 PCa patients were shown. Nineteen serum samples that are positive for anti-cyclin B1 are shown as filled black dots. The other samples are shown as unfilled circles. (b) Western blotting result of serial sera from a representative patient. Lane 1 is the sample before surgery and lanes 2 and 3 are ones after surgery.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016862&req=5

fig2: (a) Distribution of cyclin B1 antibody responses in different time points before and after surgery treatment. Anti-cyclin B1 antibody levels (OD values) in the 55 samples from 18 PCa patients were shown. Nineteen serum samples that are positive for anti-cyclin B1 are shown as filled black dots. The other samples are shown as unfilled circles. (b) Western blotting result of serial sera from a representative patient. Lane 1 is the sample before surgery and lanes 2 and 3 are ones after surgery.
Mentions: Fifty-five serial serum samples from 18 PCa patients were also detected for the expression of autoantibodies against cyclin B1. Serum samples from all these patients had been collected after the diagnosis of PCa and serum samples were also collected every three months after they had been taken surgical resection. At least two to four samples after surgery were available for each patient. These are very valuable serial serum samples from the patients with surgery and the detection of autoantibodies of these samples might reflect the prognosis after surgery. As shown in Figure 2(a), only six serum samples from 18 PCa patients before surgery reacted over the cut-off OD value of cyclin B1 autoantibody. However, 13 blood samples collected after surgery showed positive result in ELISA with higher OD value. There is likely association between cyclin B1 antibody level and the time before diagnosis. The closer to the time of diagnosis, the higher autoantibody level performed. All of the 55 serum samples were also analyzed by Western blotting to confirm the results from ELISA. Figure 2(b) shows a representative patient, who had no anti-cyclin B1 antibody before surgery (02/12/2003); however, the antibody weakly occurred six months after surgery (08/28/2003) and showed increased reactivity one year after surgery (01/22/2004).

Bottom Line: Autoantibodies to cyclin B1 and fourteen other TAAs were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting in 464 sera from patients with PCa, benign prostatic hyperplasia (BPH), and other controls.In the further analysis, 31.4% of sera from PCa patients at the early stage contained anti-cyclin B1 autoantibody, and even 29.4% of patients who had normal prostate-specific antigen (PSA) levels in their serum samples were observed anti-cyclin B1 positive.This study further supports the hypothesis that a customized TAA array can be used for enhancing anti-TAA autoantibody detection, and it may constitute a promising and powerful tool for immunodiagnosis of PCa.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USA.

ABSTRACT
Previous studies have demonstrated that sera from patients with prostate cancer (PCa) contain autoantibodies that react with tumor-associated antigens (TAAs). Autoantibodies to cyclin B1 and fourteen other TAAs were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting in 464 sera from patients with PCa, benign prostatic hyperplasia (BPH), and other controls. Autoantibodies to cyclin B1 were detected in 31.0% of sera from randomly selected patients with PCa versus 4.8% in sera with BPH. In the further analysis, 31.4% of sera from PCa patients at the early stage contained anti-cyclin B1 autoantibody, and even 29.4% of patients who had normal prostate-specific antigen (PSA) levels in their serum samples were observed anti-cyclin B1 positive. The cumulative positive rate of autoantibodies against seven selected TAAs (cyclin B1, survivin, p53, DFS70/LEDGFp75, RalA, MDM2, and NPM1) in PCa reached 80.5%, significantly higher than that in normal control sera. In summary, autoantibody to cyclin B1 might be a potential biomarker for the immunodiagnosis of early stage PCa, especially useful in patients with normal PSA level. This study further supports the hypothesis that a customized TAA array can be used for enhancing anti-TAA autoantibody detection, and it may constitute a promising and powerful tool for immunodiagnosis of PCa.

Show MeSH
Related in: MedlinePlus