Limits...
Effect of curcumin on lifespan, activity pattern, oxidative stress, and apoptosis in the brains of transgenic Drosophila model of Parkinson's disease.

Siddique YH, Naz F, Jyoti S - Biomed Res Int (2014)

Bottom Line: The effect of curcumin was studied on lifespan, activity pattern, oxidative stress, and apoptosis in the brains of PD model flies.The cell death in Drosophila brain was analyzed by isolating brains in Ringer's solution placing them in 70% ethanol and stained in acridine orange to calculate the gray scale values.Curcumin is potent in reducing PD symptoms.

View Article: PubMed Central - PubMed

Affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.

ABSTRACT

Background: A time dependent loss of dopaminergic neurons and the formation of intracellular aggregates of alpha synuclein have been reported in PD model flies.

Methods: The progeny (PD flies) expressing human alpha synuclein was exposed to 25, 50, and 100 µM of curcumin mixed in the diet for 24 days. The effect of curcumin was studied on lifespan, activity pattern, oxidative stress, and apoptosis in the brains of PD model flies. The activity of PD model flies was monitored by using Drosophila activity monitors (DAMs). For the estimation of oxidative stress, lipid peroxidation and protein carbonyl content were estimated in the flies brains of each treated groups. The cell death in Drosophila brain was analyzed by isolating brains in Ringer's solution placing them in 70% ethanol and stained in acridine orange to calculate the gray scale values.

Results: The exposure of flies to 25, 50, and 100 µM of curcumin showed a dose dependent significant delay in the loss of activity pattern, reduction in the oxidative stress and apoptosis, and increase in the life span of PD model flies.

Conclusion: Curcumin is potent in reducing PD symptoms.

Show MeSH

Related in: MedlinePlus

Effect of curcumin on survival rate measured in transgenic Drosophila melanogaster in various treated groups. (C1 = 25 μM curcumin; C2 = 50 μM curcumin; C3 = 100 μM curcumin).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4016861&req=5

fig1: Effect of curcumin on survival rate measured in transgenic Drosophila melanogaster in various treated groups. (C1 = 25 μM curcumin; C2 = 50 μM curcumin; C3 = 100 μM curcumin).

Mentions: The data collected for the male flies by Drosophila activity monitor (DAM) was analysed by chi-square periodogram. For control flies the number of peaks (significant) were more (Figures S1 (a) and (b)) compared to PD flies (Figures S2 (a) and (b)). A dose dependent significant delay in the loss of activity pattern was observed in the PD flies exposed to 25, 50, and 100 μM of curcumin (Figures S3–5, (a) and (b)). No change in the activity pattern of control flies exposed to 25, 50, and 100 μM of curcumin was observed (Figures S6–8, (a) and (b)). The PD flies exposed to 10−3 M of dopamine also showed a delay in the loss of activity (Figures S9 (a) and (b)) as compared to PD flies. The results obtained for the survival rate are shown in Figure 1. The survival rate was measured only in male flies. As is evident from Figure 1 the PD flies exposed to 25, 50, and 100 μM of curcumin showed a dose dependent significant increase in the life span as compared to unexposed PD flies. The control flies showed a life span of about 60 days. The results obtained for the estimation of lipid peroxidation are shown in Figure 2. The PD flies exposed to 25, 50, and 100 μM of curcumin showed a dose dependent significant decrease in the lipid peroxidation as compared to unexposed PD flies and control flies (Figure 2). The results obtained for protein carbonyl content are shown in Figure 3. A dose dependent significant decrease in the mean absorbance values was obtained in PD flies exposed to 25, 50, and 100 μM of curcumin as compared to unexposed PD and control flies (Figure 3). The unexposed PD flies showed the highest mean absorbance value as compared to control flies (Figure 3). The results obtained for cell death in the brains of PD flies was calculated as mean gray scale values shown in Figure 4. A significant dose dependent decrease in the mean gray scale values was obtained for the PD flies exposed to 25, 50, and 100 μM of curcumin as compared to unexposed PD and control flies (Figure 4). The PD flies exposed to 10−3 M of dopamine also showed a significant decrease in the mean gray scale value as compared to the unexposed PD flies and control flies (Figure 4).


Effect of curcumin on lifespan, activity pattern, oxidative stress, and apoptosis in the brains of transgenic Drosophila model of Parkinson's disease.

Siddique YH, Naz F, Jyoti S - Biomed Res Int (2014)

Effect of curcumin on survival rate measured in transgenic Drosophila melanogaster in various treated groups. (C1 = 25 μM curcumin; C2 = 50 μM curcumin; C3 = 100 μM curcumin).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016861&req=5

fig1: Effect of curcumin on survival rate measured in transgenic Drosophila melanogaster in various treated groups. (C1 = 25 μM curcumin; C2 = 50 μM curcumin; C3 = 100 μM curcumin).
Mentions: The data collected for the male flies by Drosophila activity monitor (DAM) was analysed by chi-square periodogram. For control flies the number of peaks (significant) were more (Figures S1 (a) and (b)) compared to PD flies (Figures S2 (a) and (b)). A dose dependent significant delay in the loss of activity pattern was observed in the PD flies exposed to 25, 50, and 100 μM of curcumin (Figures S3–5, (a) and (b)). No change in the activity pattern of control flies exposed to 25, 50, and 100 μM of curcumin was observed (Figures S6–8, (a) and (b)). The PD flies exposed to 10−3 M of dopamine also showed a delay in the loss of activity (Figures S9 (a) and (b)) as compared to PD flies. The results obtained for the survival rate are shown in Figure 1. The survival rate was measured only in male flies. As is evident from Figure 1 the PD flies exposed to 25, 50, and 100 μM of curcumin showed a dose dependent significant increase in the life span as compared to unexposed PD flies. The control flies showed a life span of about 60 days. The results obtained for the estimation of lipid peroxidation are shown in Figure 2. The PD flies exposed to 25, 50, and 100 μM of curcumin showed a dose dependent significant decrease in the lipid peroxidation as compared to unexposed PD flies and control flies (Figure 2). The results obtained for protein carbonyl content are shown in Figure 3. A dose dependent significant decrease in the mean absorbance values was obtained in PD flies exposed to 25, 50, and 100 μM of curcumin as compared to unexposed PD and control flies (Figure 3). The unexposed PD flies showed the highest mean absorbance value as compared to control flies (Figure 3). The results obtained for cell death in the brains of PD flies was calculated as mean gray scale values shown in Figure 4. A significant dose dependent decrease in the mean gray scale values was obtained for the PD flies exposed to 25, 50, and 100 μM of curcumin as compared to unexposed PD and control flies (Figure 4). The PD flies exposed to 10−3 M of dopamine also showed a significant decrease in the mean gray scale value as compared to the unexposed PD flies and control flies (Figure 4).

Bottom Line: The effect of curcumin was studied on lifespan, activity pattern, oxidative stress, and apoptosis in the brains of PD model flies.The cell death in Drosophila brain was analyzed by isolating brains in Ringer's solution placing them in 70% ethanol and stained in acridine orange to calculate the gray scale values.Curcumin is potent in reducing PD symptoms.

View Article: PubMed Central - PubMed

Affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.

ABSTRACT

Background: A time dependent loss of dopaminergic neurons and the formation of intracellular aggregates of alpha synuclein have been reported in PD model flies.

Methods: The progeny (PD flies) expressing human alpha synuclein was exposed to 25, 50, and 100 µM of curcumin mixed in the diet for 24 days. The effect of curcumin was studied on lifespan, activity pattern, oxidative stress, and apoptosis in the brains of PD model flies. The activity of PD model flies was monitored by using Drosophila activity monitors (DAMs). For the estimation of oxidative stress, lipid peroxidation and protein carbonyl content were estimated in the flies brains of each treated groups. The cell death in Drosophila brain was analyzed by isolating brains in Ringer's solution placing them in 70% ethanol and stained in acridine orange to calculate the gray scale values.

Results: The exposure of flies to 25, 50, and 100 µM of curcumin showed a dose dependent significant delay in the loss of activity pattern, reduction in the oxidative stress and apoptosis, and increase in the life span of PD model flies.

Conclusion: Curcumin is potent in reducing PD symptoms.

Show MeSH
Related in: MedlinePlus