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Wen-dan decoction improves negative emotions in sleep-deprived rats by regulating orexin-a and leptin expression.

Wu F, Song Y, Li F, He X, Ma J, Feng T, Guan B, Wang L, Li S, Liu X, Liu Y, Mao M, Liu J, Bai S, Song C - Evid Based Complement Alternat Med (2014)

Bottom Line: However, the therapeutic mechanisms of WDD remain unclear.Open field tests showed that SD increased both crossing movement (Cm) and rearing-movement (Rm) times.Orexin-A and leptin levels in blood serum increased after SD but decreased in brain compared to the control group. mRNA expressions of orexin receptor 1 and leptin receptor after SD were decreased in the prefrontal cortex but were increased in hypothalamus.

View Article: PubMed Central - PubMed

Affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 Bei Sanhuan Donglu, Chaoyang District, Beijing 100029, China.

ABSTRACT
Wen-Dan Decoction (WDD), a formula of traditional Chinese medicine, has been clinically used for treating insomnia for approximately 800 years. However, the therapeutic mechanisms of WDD remain unclear. Orexin-A plays a key role in the sleep-wake cycle, while leptin function is opposite to orexin-A. Thus, orexin-A and leptin may be important factors in sleep disorders. In this study, 48 rats were divided into control, model, WDD-treated, and diazepam-treated groups. The model of insomnia was produced by sleep deprivation (SD) for 14 days. The expressions of orexin-A, leptin, and their receptors in blood serum, prefrontal cortex, and hypothalamus were detected by enzyme-linked immunosorbent assay, immunohistochemistry, and real time PCR. Open field tests showed that SD increased both crossing movement (Cm) and rearing-movement (Rm) times. Orexin-A and leptin levels in blood serum increased after SD but decreased in brain compared to the control group. mRNA expressions of orexin receptor 1 and leptin receptor after SD were decreased in the prefrontal cortex but were increased in hypothalamus. WDD treatment normalized the behavior and upregulated orexin-A, leptin, orexin receptor 1 and leptin receptor in brain. The findings suggest that WDD treatment may regulate SD-induced negative emotions by regulating orexin-A and leptin expression.

No MeSH data available.


Related in: MedlinePlus

The effect of sleep deprivation on orexin-A concentrations in serum and the regulation of Wen-Dan Decoction. **P < 0.01: versus the control group; ▲P < 0.05: versus the model group. Data are presented as mean ± standard error of the mean (SEM).
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fig5: The effect of sleep deprivation on orexin-A concentrations in serum and the regulation of Wen-Dan Decoction. **P < 0.01: versus the control group; ▲P < 0.05: versus the model group. Data are presented as mean ± standard error of the mean (SEM).

Mentions: Serum levels of orexin-A in the model group were significantly higher than those in the control group (P < 0.01), while serum levels of orexin-A in the WDD group were lower than those of the model group (P < 0.05) (Figure 5). Leptin serum concentrations in the model group showed no significant differences from those of the control group (P > 0.01). (Figure 6).


Wen-dan decoction improves negative emotions in sleep-deprived rats by regulating orexin-a and leptin expression.

Wu F, Song Y, Li F, He X, Ma J, Feng T, Guan B, Wang L, Li S, Liu X, Liu Y, Mao M, Liu J, Bai S, Song C - Evid Based Complement Alternat Med (2014)

The effect of sleep deprivation on orexin-A concentrations in serum and the regulation of Wen-Dan Decoction. **P < 0.01: versus the control group; ▲P < 0.05: versus the model group. Data are presented as mean ± standard error of the mean (SEM).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016855&req=5

fig5: The effect of sleep deprivation on orexin-A concentrations in serum and the regulation of Wen-Dan Decoction. **P < 0.01: versus the control group; ▲P < 0.05: versus the model group. Data are presented as mean ± standard error of the mean (SEM).
Mentions: Serum levels of orexin-A in the model group were significantly higher than those in the control group (P < 0.01), while serum levels of orexin-A in the WDD group were lower than those of the model group (P < 0.05) (Figure 5). Leptin serum concentrations in the model group showed no significant differences from those of the control group (P > 0.01). (Figure 6).

Bottom Line: However, the therapeutic mechanisms of WDD remain unclear.Open field tests showed that SD increased both crossing movement (Cm) and rearing-movement (Rm) times.Orexin-A and leptin levels in blood serum increased after SD but decreased in brain compared to the control group. mRNA expressions of orexin receptor 1 and leptin receptor after SD were decreased in the prefrontal cortex but were increased in hypothalamus.

View Article: PubMed Central - PubMed

Affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 Bei Sanhuan Donglu, Chaoyang District, Beijing 100029, China.

ABSTRACT
Wen-Dan Decoction (WDD), a formula of traditional Chinese medicine, has been clinically used for treating insomnia for approximately 800 years. However, the therapeutic mechanisms of WDD remain unclear. Orexin-A plays a key role in the sleep-wake cycle, while leptin function is opposite to orexin-A. Thus, orexin-A and leptin may be important factors in sleep disorders. In this study, 48 rats were divided into control, model, WDD-treated, and diazepam-treated groups. The model of insomnia was produced by sleep deprivation (SD) for 14 days. The expressions of orexin-A, leptin, and their receptors in blood serum, prefrontal cortex, and hypothalamus were detected by enzyme-linked immunosorbent assay, immunohistochemistry, and real time PCR. Open field tests showed that SD increased both crossing movement (Cm) and rearing-movement (Rm) times. Orexin-A and leptin levels in blood serum increased after SD but decreased in brain compared to the control group. mRNA expressions of orexin receptor 1 and leptin receptor after SD were decreased in the prefrontal cortex but were increased in hypothalamus. WDD treatment normalized the behavior and upregulated orexin-A, leptin, orexin receptor 1 and leptin receptor in brain. The findings suggest that WDD treatment may regulate SD-induced negative emotions by regulating orexin-A and leptin expression.

No MeSH data available.


Related in: MedlinePlus