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Wen-dan decoction improves negative emotions in sleep-deprived rats by regulating orexin-a and leptin expression.

Wu F, Song Y, Li F, He X, Ma J, Feng T, Guan B, Wang L, Li S, Liu X, Liu Y, Mao M, Liu J, Bai S, Song C - Evid Based Complement Alternat Med (2014)

Bottom Line: However, the therapeutic mechanisms of WDD remain unclear.Open field tests showed that SD increased both crossing movement (Cm) and rearing-movement (Rm) times.Orexin-A and leptin levels in blood serum increased after SD but decreased in brain compared to the control group. mRNA expressions of orexin receptor 1 and leptin receptor after SD were decreased in the prefrontal cortex but were increased in hypothalamus.

View Article: PubMed Central - PubMed

Affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 Bei Sanhuan Donglu, Chaoyang District, Beijing 100029, China.

ABSTRACT
Wen-Dan Decoction (WDD), a formula of traditional Chinese medicine, has been clinically used for treating insomnia for approximately 800 years. However, the therapeutic mechanisms of WDD remain unclear. Orexin-A plays a key role in the sleep-wake cycle, while leptin function is opposite to orexin-A. Thus, orexin-A and leptin may be important factors in sleep disorders. In this study, 48 rats were divided into control, model, WDD-treated, and diazepam-treated groups. The model of insomnia was produced by sleep deprivation (SD) for 14 days. The expressions of orexin-A, leptin, and their receptors in blood serum, prefrontal cortex, and hypothalamus were detected by enzyme-linked immunosorbent assay, immunohistochemistry, and real time PCR. Open field tests showed that SD increased both crossing movement (Cm) and rearing-movement (Rm) times. Orexin-A and leptin levels in blood serum increased after SD but decreased in brain compared to the control group. mRNA expressions of orexin receptor 1 and leptin receptor after SD were decreased in the prefrontal cortex but were increased in hypothalamus. WDD treatment normalized the behavior and upregulated orexin-A, leptin, orexin receptor 1 and leptin receptor in brain. The findings suggest that WDD treatment may regulate SD-induced negative emotions by regulating orexin-A and leptin expression.

No MeSH data available.


Related in: MedlinePlus

Protein expression of orexin-A in hypothalamus. (Tissue sections were viewed at 40x magnification.) The claybank cells, which are positive for orexin-A expression, are oval-shaped and mainly spread throughout the endochylema in hypothalamus. Fewer positive cells are more apparent in the model group and the diazepam- and WDD-treated groups than the control group.
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fig2: Protein expression of orexin-A in hypothalamus. (Tissue sections were viewed at 40x magnification.) The claybank cells, which are positive for orexin-A expression, are oval-shaped and mainly spread throughout the endochylema in hypothalamus. Fewer positive cells are more apparent in the model group and the diazepam- and WDD-treated groups than the control group.

Mentions: In both prefrontal cortex and hypothalamus, orexin-A and leptin expressions in the model group were significantly lower than those in the control group (P < 0.01) (Figures 1, 2, 3, and 4). Remarkably, the expressions of orexin-A and leptin in the WDD group were significantly higher than those in the model group (P < 0.010). Compared to the diazepam group, the expressions of both orexin-A and leptin in the WDD group were significantly higher (P < 0.05) (Table 4).


Wen-dan decoction improves negative emotions in sleep-deprived rats by regulating orexin-a and leptin expression.

Wu F, Song Y, Li F, He X, Ma J, Feng T, Guan B, Wang L, Li S, Liu X, Liu Y, Mao M, Liu J, Bai S, Song C - Evid Based Complement Alternat Med (2014)

Protein expression of orexin-A in hypothalamus. (Tissue sections were viewed at 40x magnification.) The claybank cells, which are positive for orexin-A expression, are oval-shaped and mainly spread throughout the endochylema in hypothalamus. Fewer positive cells are more apparent in the model group and the diazepam- and WDD-treated groups than the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016855&req=5

fig2: Protein expression of orexin-A in hypothalamus. (Tissue sections were viewed at 40x magnification.) The claybank cells, which are positive for orexin-A expression, are oval-shaped and mainly spread throughout the endochylema in hypothalamus. Fewer positive cells are more apparent in the model group and the diazepam- and WDD-treated groups than the control group.
Mentions: In both prefrontal cortex and hypothalamus, orexin-A and leptin expressions in the model group were significantly lower than those in the control group (P < 0.01) (Figures 1, 2, 3, and 4). Remarkably, the expressions of orexin-A and leptin in the WDD group were significantly higher than those in the model group (P < 0.010). Compared to the diazepam group, the expressions of both orexin-A and leptin in the WDD group were significantly higher (P < 0.05) (Table 4).

Bottom Line: However, the therapeutic mechanisms of WDD remain unclear.Open field tests showed that SD increased both crossing movement (Cm) and rearing-movement (Rm) times.Orexin-A and leptin levels in blood serum increased after SD but decreased in brain compared to the control group. mRNA expressions of orexin receptor 1 and leptin receptor after SD were decreased in the prefrontal cortex but were increased in hypothalamus.

View Article: PubMed Central - PubMed

Affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 Bei Sanhuan Donglu, Chaoyang District, Beijing 100029, China.

ABSTRACT
Wen-Dan Decoction (WDD), a formula of traditional Chinese medicine, has been clinically used for treating insomnia for approximately 800 years. However, the therapeutic mechanisms of WDD remain unclear. Orexin-A plays a key role in the sleep-wake cycle, while leptin function is opposite to orexin-A. Thus, orexin-A and leptin may be important factors in sleep disorders. In this study, 48 rats were divided into control, model, WDD-treated, and diazepam-treated groups. The model of insomnia was produced by sleep deprivation (SD) for 14 days. The expressions of orexin-A, leptin, and their receptors in blood serum, prefrontal cortex, and hypothalamus were detected by enzyme-linked immunosorbent assay, immunohistochemistry, and real time PCR. Open field tests showed that SD increased both crossing movement (Cm) and rearing-movement (Rm) times. Orexin-A and leptin levels in blood serum increased after SD but decreased in brain compared to the control group. mRNA expressions of orexin receptor 1 and leptin receptor after SD were decreased in the prefrontal cortex but were increased in hypothalamus. WDD treatment normalized the behavior and upregulated orexin-A, leptin, orexin receptor 1 and leptin receptor in brain. The findings suggest that WDD treatment may regulate SD-induced negative emotions by regulating orexin-A and leptin expression.

No MeSH data available.


Related in: MedlinePlus