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The multiple silicone tube device, "tubes within a tube," for multiplication in nerve reconstruction.

Johansson F, Dahlin LB - Biomed Res Int (2014)

Bottom Line: Transected rat sciatic nerve stumps were inserted into a nine mm long silicone tube, which contained four, five mm long, smaller tubes, thus leaving a five mm gap for regenerating nerve fibers.Stimulation of the proximal nerve elicited contractions in distal muscles.Thin metal electrodes, inserted initially into the smaller tubes in some experiments, became embedded in the new nerve structures and when stimulated contractions of the distal muscles were observed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology/Functional Zoology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden.

ABSTRACT
Multiple nerve branches were created during the regeneration procedure after a nerve injury and such multiple branches are suggested to be used to control, for example, prosthesis with many degrees of freedom. Transected rat sciatic nerve stumps were inserted into a nine mm long silicone tube, which contained four, five mm long, smaller tubes, thus leaving a five mm gap for regenerating nerve fibers. Six weeks later, several new nerve structures were formed not only in the four smaller tubes, but also in the spaces in-between. The 7-9 new continuous nerve structures, which were isolated as individual free nerves after removal of the tubes, were delineated by a perineurium and contained both myelinated and unmyelinated nerve fibers as well as blood vessels. Stimulation of the proximal nerve elicited contractions in distal muscles. Thin metal electrodes, inserted initially into the smaller tubes in some experiments, became embedded in the new nerve structures and when stimulated contractions of the distal muscles were observed. The "tubes within a tube" technique, creating multiple new nerves from a single "mother" nerve, can be used to record multiple signals for prosthetic device control or as sources for supply of multiple denervated targets.

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Related in: MedlinePlus

Dissected preparation. The large and smaller tubes have been removed and the new nerve structures lay bare. Eight new nerve structures are visible.
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fig4: Dissected preparation. The large and smaller tubes have been removed and the new nerve structures lay bare. Eight new nerve structures are visible.

Mentions: Structures had been formed within the tubes joining the two cut ends of the sciatic nerve (Figure 3). Removal of the outer and inner tubes showed that there were a total of seven to nine new small nerve trunks in the tube within tube chamber (Figures 4 and 5). After sectioning, the new nerves could be studied in the light microscope. The nerve structure was either round or irregular depending on if they were formed within a tube or formed within an area between the tubes. A perineurium-like sheath surrounded the small nerve trunks (Figure 6(a)). In the endoneurium, blood vessels and myelinated axons were readily observable. Immunostaining for neurofilaments showed that there were also unmyelinated fibers in the endoneurium of the individual new small nerve trunks (Figure 6(b)). The counterstaining with bisbenzimide for nuclei identification revealed an apparent normal distribution of perineurial nuclei, Schwann cell nuclei, and endothelial cell nuclei in the blood vessels (Figure 6(c)). There was an empty space left by the electrode, which was surrounded by mononuclear inflammatory cells (Figure 7).


The multiple silicone tube device, "tubes within a tube," for multiplication in nerve reconstruction.

Johansson F, Dahlin LB - Biomed Res Int (2014)

Dissected preparation. The large and smaller tubes have been removed and the new nerve structures lay bare. Eight new nerve structures are visible.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016851&req=5

fig4: Dissected preparation. The large and smaller tubes have been removed and the new nerve structures lay bare. Eight new nerve structures are visible.
Mentions: Structures had been formed within the tubes joining the two cut ends of the sciatic nerve (Figure 3). Removal of the outer and inner tubes showed that there were a total of seven to nine new small nerve trunks in the tube within tube chamber (Figures 4 and 5). After sectioning, the new nerves could be studied in the light microscope. The nerve structure was either round or irregular depending on if they were formed within a tube or formed within an area between the tubes. A perineurium-like sheath surrounded the small nerve trunks (Figure 6(a)). In the endoneurium, blood vessels and myelinated axons were readily observable. Immunostaining for neurofilaments showed that there were also unmyelinated fibers in the endoneurium of the individual new small nerve trunks (Figure 6(b)). The counterstaining with bisbenzimide for nuclei identification revealed an apparent normal distribution of perineurial nuclei, Schwann cell nuclei, and endothelial cell nuclei in the blood vessels (Figure 6(c)). There was an empty space left by the electrode, which was surrounded by mononuclear inflammatory cells (Figure 7).

Bottom Line: Transected rat sciatic nerve stumps were inserted into a nine mm long silicone tube, which contained four, five mm long, smaller tubes, thus leaving a five mm gap for regenerating nerve fibers.Stimulation of the proximal nerve elicited contractions in distal muscles.Thin metal electrodes, inserted initially into the smaller tubes in some experiments, became embedded in the new nerve structures and when stimulated contractions of the distal muscles were observed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology/Functional Zoology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden.

ABSTRACT
Multiple nerve branches were created during the regeneration procedure after a nerve injury and such multiple branches are suggested to be used to control, for example, prosthesis with many degrees of freedom. Transected rat sciatic nerve stumps were inserted into a nine mm long silicone tube, which contained four, five mm long, smaller tubes, thus leaving a five mm gap for regenerating nerve fibers. Six weeks later, several new nerve structures were formed not only in the four smaller tubes, but also in the spaces in-between. The 7-9 new continuous nerve structures, which were isolated as individual free nerves after removal of the tubes, were delineated by a perineurium and contained both myelinated and unmyelinated nerve fibers as well as blood vessels. Stimulation of the proximal nerve elicited contractions in distal muscles. Thin metal electrodes, inserted initially into the smaller tubes in some experiments, became embedded in the new nerve structures and when stimulated contractions of the distal muscles were observed. The "tubes within a tube" technique, creating multiple new nerves from a single "mother" nerve, can be used to record multiple signals for prosthetic device control or as sources for supply of multiple denervated targets.

Show MeSH
Related in: MedlinePlus