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The multiple silicone tube device, "tubes within a tube," for multiplication in nerve reconstruction.

Johansson F, Dahlin LB - Biomed Res Int (2014)

Bottom Line: Transected rat sciatic nerve stumps were inserted into a nine mm long silicone tube, which contained four, five mm long, smaller tubes, thus leaving a five mm gap for regenerating nerve fibers.Stimulation of the proximal nerve elicited contractions in distal muscles.Thin metal electrodes, inserted initially into the smaller tubes in some experiments, became embedded in the new nerve structures and when stimulated contractions of the distal muscles were observed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology/Functional Zoology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden.

ABSTRACT
Multiple nerve branches were created during the regeneration procedure after a nerve injury and such multiple branches are suggested to be used to control, for example, prosthesis with many degrees of freedom. Transected rat sciatic nerve stumps were inserted into a nine mm long silicone tube, which contained four, five mm long, smaller tubes, thus leaving a five mm gap for regenerating nerve fibers. Six weeks later, several new nerve structures were formed not only in the four smaller tubes, but also in the spaces in-between. The 7-9 new continuous nerve structures, which were isolated as individual free nerves after removal of the tubes, were delineated by a perineurium and contained both myelinated and unmyelinated nerve fibers as well as blood vessels. Stimulation of the proximal nerve elicited contractions in distal muscles. Thin metal electrodes, inserted initially into the smaller tubes in some experiments, became embedded in the new nerve structures and when stimulated contractions of the distal muscles were observed. The "tubes within a tube" technique, creating multiple new nerves from a single "mother" nerve, can be used to record multiple signals for prosthetic device control or as sources for supply of multiple denervated targets.

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Related in: MedlinePlus

Large picture: needles (0.4 mm) contacting electrode leads for stimulation via the Pt/Ir electrodes in the smaller tubes. Upper insert: stimulator delivering rectangular pulses of 1 ms duration at 10 Hz and different amplitudes (0.1–1 V). Lower insert: electrodes needles (0.7 mm) for stimulation of the sciatic nerve proximal to the tube repair site.
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fig2: Large picture: needles (0.4 mm) contacting electrode leads for stimulation via the Pt/Ir electrodes in the smaller tubes. Upper insert: stimulator delivering rectangular pulses of 1 ms duration at 10 Hz and different amplitudes (0.1–1 V). Lower insert: electrodes needles (0.7 mm) for stimulation of the sciatic nerve proximal to the tube repair site.

Mentions: At the day of evaluation, the animals were reanesthetized and the tube, with the attached sciatic nerve, was exposed. Needle electrodes were used for stimulation, providing square pulses of 10 Hz, 1 ms duration with an amplitude of 0.2 V to 1 V. The stimulation was delivered either proximal to the chamber or via the leads to the internal electrodes of the smaller tubes (Figure 2). Visual observation of flexion of the foot or contraction distal to the site of repair at a frequency of the stimulation, that is, 10 Hz, would signify that axons had regenerated through the tubes into the distal muscles. In some experiments, the undamaged contralateral sciatic nerves were exposed to determine the threshold for muscle contraction.


The multiple silicone tube device, "tubes within a tube," for multiplication in nerve reconstruction.

Johansson F, Dahlin LB - Biomed Res Int (2014)

Large picture: needles (0.4 mm) contacting electrode leads for stimulation via the Pt/Ir electrodes in the smaller tubes. Upper insert: stimulator delivering rectangular pulses of 1 ms duration at 10 Hz and different amplitudes (0.1–1 V). Lower insert: electrodes needles (0.7 mm) for stimulation of the sciatic nerve proximal to the tube repair site.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016851&req=5

fig2: Large picture: needles (0.4 mm) contacting electrode leads for stimulation via the Pt/Ir electrodes in the smaller tubes. Upper insert: stimulator delivering rectangular pulses of 1 ms duration at 10 Hz and different amplitudes (0.1–1 V). Lower insert: electrodes needles (0.7 mm) for stimulation of the sciatic nerve proximal to the tube repair site.
Mentions: At the day of evaluation, the animals were reanesthetized and the tube, with the attached sciatic nerve, was exposed. Needle electrodes were used for stimulation, providing square pulses of 10 Hz, 1 ms duration with an amplitude of 0.2 V to 1 V. The stimulation was delivered either proximal to the chamber or via the leads to the internal electrodes of the smaller tubes (Figure 2). Visual observation of flexion of the foot or contraction distal to the site of repair at a frequency of the stimulation, that is, 10 Hz, would signify that axons had regenerated through the tubes into the distal muscles. In some experiments, the undamaged contralateral sciatic nerves were exposed to determine the threshold for muscle contraction.

Bottom Line: Transected rat sciatic nerve stumps were inserted into a nine mm long silicone tube, which contained four, five mm long, smaller tubes, thus leaving a five mm gap for regenerating nerve fibers.Stimulation of the proximal nerve elicited contractions in distal muscles.Thin metal electrodes, inserted initially into the smaller tubes in some experiments, became embedded in the new nerve structures and when stimulated contractions of the distal muscles were observed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology/Functional Zoology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden.

ABSTRACT
Multiple nerve branches were created during the regeneration procedure after a nerve injury and such multiple branches are suggested to be used to control, for example, prosthesis with many degrees of freedom. Transected rat sciatic nerve stumps were inserted into a nine mm long silicone tube, which contained four, five mm long, smaller tubes, thus leaving a five mm gap for regenerating nerve fibers. Six weeks later, several new nerve structures were formed not only in the four smaller tubes, but also in the spaces in-between. The 7-9 new continuous nerve structures, which were isolated as individual free nerves after removal of the tubes, were delineated by a perineurium and contained both myelinated and unmyelinated nerve fibers as well as blood vessels. Stimulation of the proximal nerve elicited contractions in distal muscles. Thin metal electrodes, inserted initially into the smaller tubes in some experiments, became embedded in the new nerve structures and when stimulated contractions of the distal muscles were observed. The "tubes within a tube" technique, creating multiple new nerves from a single "mother" nerve, can be used to record multiple signals for prosthetic device control or as sources for supply of multiple denervated targets.

Show MeSH
Related in: MedlinePlus