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Topical application of Cleome viscosa increases the expression of basic fibroblast growth factor and type III collagen in rat cutaneous wound.

Upadhyay A, Chattopadhyay P, Goyary D, Mazumder PM, Veer V - Biomed Res Int (2014)

Bottom Line: The plant is also known as Asian spider flower and is distributed throughout the greater part of India.Western blot for COL3A1, bFGF, and Smad-2, Smad-3, Smad-4, and Smad-7 was performed with 7-day postoperative granulation tissue.Western blot analysis revealed that CvME significantly upregulated the expression of COL3A1 and bFGF and increased the Smad-mediated collagen production in granulation tissue.

View Article: PubMed Central - PubMed

Affiliation: Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam 784001, India ; Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835215, India.

ABSTRACT
Cleome viscosa L. (Cleomaceae) is an important traditional medicine of the Indian-Ayurvedic and Chinese-medicine system documented for rheumatic arthritis, hypertension, malaria, neurasthenia, and wound healing. The plant is also known as Asian spider flower and is distributed throughout the greater part of India. The present study explored the wound healing property of C. viscosa methanol extract (CvME) and its related mechanism using Wistar rat cutaneous excision wound model. Wound contraction rate, hydroxyproline quantification, and histopathological examination of wound granulation tissue were performed. The healing potential was comparatively assessed with a reference gentamicin sulfate hydrogel (0.01% w/w). Western blot for COL3A1, bFGF, and Smad-2, Smad-3, Smad-4, and Smad-7 was performed with 7-day postoperative granulation tissue. Results revealed that the topical application of CvME (2.5% w/w) significantly accelerated the wound contraction rate (95.14%, 24 postoperative days), increased the hydroxyproline content (3.947 mg/100 mg tissue), and improved histopathology of wound tissue as compared to control groups. Western blot analysis revealed that CvME significantly upregulated the expression of COL3A1 and bFGF and increased the Smad-mediated collagen production in granulation tissue. These findings suggest that C. viscosa promoted the wound repair process by attenuating the Smad-mediated collagen production in wound granulation tissue.

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Effect of C. viscosa methanol extract (CvME) on COL3A1, bFGF, and Smad-2, Smad-3, Smad-4, and Smad-7 protein expressions on day 7 (seven) in wound tissues, detected by Western blot. Lane (1) represents nontreated, (2) vehicle control, and (3) CvME and (4) gentamicin sulfate treated animal group, respectively. Values are expressed as mean ± SD. Asterisk (∗) indicates significantly different P < 0.05 as compared to the nontreated and vehicle treated groups. Hash (#) indicates significantly different P < 0.05 as compared to gentamicin sulfate treated group.
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fig4: Effect of C. viscosa methanol extract (CvME) on COL3A1, bFGF, and Smad-2, Smad-3, Smad-4, and Smad-7 protein expressions on day 7 (seven) in wound tissues, detected by Western blot. Lane (1) represents nontreated, (2) vehicle control, and (3) CvME and (4) gentamicin sulfate treated animal group, respectively. Values are expressed as mean ± SD. Asterisk (∗) indicates significantly different P < 0.05 as compared to the nontreated and vehicle treated groups. Hash (#) indicates significantly different P < 0.05 as compared to gentamicin sulfate treated group.

Mentions: Proliferation phase of wound healing involves formation and vascularization of granulation tissue, collagen production, and their subsequent maturation. Collagen type III, present in remodeling wound tissues, is produced by the proliferating young fibroblasts under the influence of various growth factors like transforming growth factor-β (TGF-β), basic fibroblast growth factor (bFGF), and so forth. The bFGF is a potent angiogenic molecule, which promotes the neovascularization and blood supply to the granulation tissue. Increased oxygen supply facilitates the maturation ofcollagen fibers in granulation tissue [3]. Western blot analysis revealed that application of CvME significantly increased (P < 0.05) the expression of COL3A1 and bFGF protein in wound granulation tissue (Figure 4), thus further confirming the above findings of faster wound contraction rate and higher hydroxyproline content.


Topical application of Cleome viscosa increases the expression of basic fibroblast growth factor and type III collagen in rat cutaneous wound.

Upadhyay A, Chattopadhyay P, Goyary D, Mazumder PM, Veer V - Biomed Res Int (2014)

Effect of C. viscosa methanol extract (CvME) on COL3A1, bFGF, and Smad-2, Smad-3, Smad-4, and Smad-7 protein expressions on day 7 (seven) in wound tissues, detected by Western blot. Lane (1) represents nontreated, (2) vehicle control, and (3) CvME and (4) gentamicin sulfate treated animal group, respectively. Values are expressed as mean ± SD. Asterisk (∗) indicates significantly different P < 0.05 as compared to the nontreated and vehicle treated groups. Hash (#) indicates significantly different P < 0.05 as compared to gentamicin sulfate treated group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016850&req=5

fig4: Effect of C. viscosa methanol extract (CvME) on COL3A1, bFGF, and Smad-2, Smad-3, Smad-4, and Smad-7 protein expressions on day 7 (seven) in wound tissues, detected by Western blot. Lane (1) represents nontreated, (2) vehicle control, and (3) CvME and (4) gentamicin sulfate treated animal group, respectively. Values are expressed as mean ± SD. Asterisk (∗) indicates significantly different P < 0.05 as compared to the nontreated and vehicle treated groups. Hash (#) indicates significantly different P < 0.05 as compared to gentamicin sulfate treated group.
Mentions: Proliferation phase of wound healing involves formation and vascularization of granulation tissue, collagen production, and their subsequent maturation. Collagen type III, present in remodeling wound tissues, is produced by the proliferating young fibroblasts under the influence of various growth factors like transforming growth factor-β (TGF-β), basic fibroblast growth factor (bFGF), and so forth. The bFGF is a potent angiogenic molecule, which promotes the neovascularization and blood supply to the granulation tissue. Increased oxygen supply facilitates the maturation ofcollagen fibers in granulation tissue [3]. Western blot analysis revealed that application of CvME significantly increased (P < 0.05) the expression of COL3A1 and bFGF protein in wound granulation tissue (Figure 4), thus further confirming the above findings of faster wound contraction rate and higher hydroxyproline content.

Bottom Line: The plant is also known as Asian spider flower and is distributed throughout the greater part of India.Western blot for COL3A1, bFGF, and Smad-2, Smad-3, Smad-4, and Smad-7 was performed with 7-day postoperative granulation tissue.Western blot analysis revealed that CvME significantly upregulated the expression of COL3A1 and bFGF and increased the Smad-mediated collagen production in granulation tissue.

View Article: PubMed Central - PubMed

Affiliation: Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam 784001, India ; Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835215, India.

ABSTRACT
Cleome viscosa L. (Cleomaceae) is an important traditional medicine of the Indian-Ayurvedic and Chinese-medicine system documented for rheumatic arthritis, hypertension, malaria, neurasthenia, and wound healing. The plant is also known as Asian spider flower and is distributed throughout the greater part of India. The present study explored the wound healing property of C. viscosa methanol extract (CvME) and its related mechanism using Wistar rat cutaneous excision wound model. Wound contraction rate, hydroxyproline quantification, and histopathological examination of wound granulation tissue were performed. The healing potential was comparatively assessed with a reference gentamicin sulfate hydrogel (0.01% w/w). Western blot for COL3A1, bFGF, and Smad-2, Smad-3, Smad-4, and Smad-7 was performed with 7-day postoperative granulation tissue. Results revealed that the topical application of CvME (2.5% w/w) significantly accelerated the wound contraction rate (95.14%, 24 postoperative days), increased the hydroxyproline content (3.947 mg/100 mg tissue), and improved histopathology of wound tissue as compared to control groups. Western blot analysis revealed that CvME significantly upregulated the expression of COL3A1 and bFGF and increased the Smad-mediated collagen production in granulation tissue. These findings suggest that C. viscosa promoted the wound repair process by attenuating the Smad-mediated collagen production in wound granulation tissue.

Show MeSH
Related in: MedlinePlus