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A Platform for Screening Potential Anticholinesterase Fractions and Components Obtained from Anemarrhena asphodeloides Bge for Treating Alzheimer's Disease.

Sun Y, Peng Y, Li LG, Zheng LW, Lin DJ, Li LZ, Song SJ - Evid Based Complement Alternat Med (2014)

Bottom Line: The results showed that compounds with the C6-C3-C6 skeleton probably had both AChE and BuChE inhibitory activities.Lignans showed weak BuChE inhibitory activity.The steroidal saponins demonstrated moderate or weak AChE inhibitory activity.

View Article: PubMed Central - PubMed

Affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.

ABSTRACT
Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive memory loss and cognitive impairment. Cholinesterase inhibitors are widely used for the symptomatic treatment of Alzheimer's disease to enhance central cholinergic transmission. In this study, a bioactivity-oriented screening platform based on a modified Ellman's method and HPLC-QTOF MS technique was developed to rapidly screen active agents of Anemarrhena asphodeloides Bge. The 60% ethanol fraction from an ethyl acetate extract exhibited the most potential anticholinesterase activity. Fifteen steroid saponins were identified by the mass spectrum, standards and literature reports. Twenty-five compounds were isolated from the active fraction. The results showed that compounds with the C6-C3-C6 skeleton probably had both AChE and BuChE inhibitory activities. Xanthone and benzene derivatives exhibited no or little activity. Lignans showed weak BuChE inhibitory activity. The steroidal saponins demonstrated moderate or weak AChE inhibitory activity.

No MeSH data available.


Related in: MedlinePlus

MS spectra of the authentic standards.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig4: MS spectra of the authentic standards.

Mentions: To obtain the information about precursor ions and characteristic fragment ions of the compounds, six authentic standards were injected into the LC-MS system. The fragmentation patterns for these authentic standards were discussed in detail below. MS spectra of the authentic standards were shown in Figure 4.


A Platform for Screening Potential Anticholinesterase Fractions and Components Obtained from Anemarrhena asphodeloides Bge for Treating Alzheimer's Disease.

Sun Y, Peng Y, Li LG, Zheng LW, Lin DJ, Li LZ, Song SJ - Evid Based Complement Alternat Med (2014)

MS spectra of the authentic standards.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016847&req=5

fig4: MS spectra of the authentic standards.
Mentions: To obtain the information about precursor ions and characteristic fragment ions of the compounds, six authentic standards were injected into the LC-MS system. The fragmentation patterns for these authentic standards were discussed in detail below. MS spectra of the authentic standards were shown in Figure 4.

Bottom Line: The results showed that compounds with the C6-C3-C6 skeleton probably had both AChE and BuChE inhibitory activities.Lignans showed weak BuChE inhibitory activity.The steroidal saponins demonstrated moderate or weak AChE inhibitory activity.

View Article: PubMed Central - PubMed

Affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.

ABSTRACT
Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive memory loss and cognitive impairment. Cholinesterase inhibitors are widely used for the symptomatic treatment of Alzheimer's disease to enhance central cholinergic transmission. In this study, a bioactivity-oriented screening platform based on a modified Ellman's method and HPLC-QTOF MS technique was developed to rapidly screen active agents of Anemarrhena asphodeloides Bge. The 60% ethanol fraction from an ethyl acetate extract exhibited the most potential anticholinesterase activity. Fifteen steroid saponins were identified by the mass spectrum, standards and literature reports. Twenty-five compounds were isolated from the active fraction. The results showed that compounds with the C6-C3-C6 skeleton probably had both AChE and BuChE inhibitory activities. Xanthone and benzene derivatives exhibited no or little activity. Lignans showed weak BuChE inhibitory activity. The steroidal saponins demonstrated moderate or weak AChE inhibitory activity.

No MeSH data available.


Related in: MedlinePlus