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Biosynthesis and Antimicrobial Activity of Semiconductor Nanoparticles against Oral Pathogens.

Malarkodi C, Rajeshkumar S, Paulkumar K, Vanaja M, Gnanajobitha G, Annadurai G - Bioinorg Chem Appl (2014)

Bottom Line: The results indicated that the proteins, which contain amine groups, played a reducing and controlling responsibility during the formation of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles in the colloidal solution.Staphylococcus sp.Lactobacillus sp., and Candida albicans and these results confirmed that the sulphide nanoparticles are exhibiting good bactericidal activity.

View Article: PubMed Central - PubMed

Affiliation: Environmental Nanotechnology Division, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, Tamilnadu 627412, India.

ABSTRACT
Dental care is an essential phenomenon in human health. Oral pathogens can cause severe break which may show the way to serious issues in human disease like blood circulation and coronary disease. In the current study, we demonstrated the synthesis and antimicrobial activity of cadmium sulphide and zinc sulphide nanoparticles against oral pathogens. The process for the synthesis of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles is fast, novel, and ecofriendly. Formation of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles was confirmed by surface plasmon spectra using UV-Vis spectrophotometer. The morphology of crystalline phase of nanoparticles was determined from transmission electron microscopy (TEM) and X-ray diffraction (XRD) spectra. The average size of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles was in the range of 10 nm to 25 nm and 65 nm, respectively, and the observed morphology was spherical. The results indicated that the proteins, which contain amine groups, played a reducing and controlling responsibility during the formation of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles in the colloidal solution. The antimicrobial activity was assessed against oral pathogens such as Streptococcus sp. Staphylococcus sp. Lactobacillus sp., and Candida albicans and these results confirmed that the sulphide nanoparticles are exhibiting good bactericidal activity.

No MeSH data available.


Related in: MedlinePlus

Probable pathway of biosynthesis and stability of biofunctionalized sulfide nanoparticle.
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fig6: Probable pathway of biosynthesis and stability of biofunctionalized sulfide nanoparticle.

Mentions: The present study reports the biological synthesis of sulphide nanoparticles like CdS and ZnS using the bacteria K. pneumoniae. Aiking et al. (1982) have been reported that the Klebsiella aerogens has undergone two different detoxification processes against sulphate and phosphate such as metal sulphide and metal phosphate formation. Additionally, they suggested that the cadmium ions could be detoxified by the formation of extracellular cadmium sulphide particles [38–40]. Similarly, herein, K. pneumoniae detoxifies the cadmium sulphate and zinc sulphate and synthesizes the nanosized CdS and ZnS particles, respectively. In synthesis process (Figure 6), first, the sulphate ions are taken in (SO42−) from the extracellular environment (nutrient broth medium) and reduced to adenosine phosphosulphate with the support of the enzyme ATP sulfurylase [40]. The adenosine phosphosulphate further phosphorylated to form 3′phosphoadenosine phosphosulphate. The 3′phosphoadenosine phosphosulphate is reduced to form sulphite ions (SO32−) with the assistance of phosphoadenosine phosphosulphate reductase [39]. Then, the sulphite ions are reduced to sulphide ions (S2−) with the help of sulphite reductase. The reduced sulphide ions are coupled with inorganic metal ions cadmium and zinc in extracellular environment and resulted in the formation of cadmium sulphide and zinc sulphide nanoparticles [40, 41]. Likewise sulphate ions, the selenium ions are also reduced to selenide ions through enzymatic reaction. Previously, Li et al. (2007) have demonstrated the reduction of selenium ions with the assistance of 30 kDa protein molecules present in the extract of Capsicum annuum [42]. The presence of phytochemicals and secondary metabolites in the plant extracts also have the capability to synthesis inorganic nanoparticles [43].


Biosynthesis and Antimicrobial Activity of Semiconductor Nanoparticles against Oral Pathogens.

Malarkodi C, Rajeshkumar S, Paulkumar K, Vanaja M, Gnanajobitha G, Annadurai G - Bioinorg Chem Appl (2014)

Probable pathway of biosynthesis and stability of biofunctionalized sulfide nanoparticle.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016845&req=5

fig6: Probable pathway of biosynthesis and stability of biofunctionalized sulfide nanoparticle.
Mentions: The present study reports the biological synthesis of sulphide nanoparticles like CdS and ZnS using the bacteria K. pneumoniae. Aiking et al. (1982) have been reported that the Klebsiella aerogens has undergone two different detoxification processes against sulphate and phosphate such as metal sulphide and metal phosphate formation. Additionally, they suggested that the cadmium ions could be detoxified by the formation of extracellular cadmium sulphide particles [38–40]. Similarly, herein, K. pneumoniae detoxifies the cadmium sulphate and zinc sulphate and synthesizes the nanosized CdS and ZnS particles, respectively. In synthesis process (Figure 6), first, the sulphate ions are taken in (SO42−) from the extracellular environment (nutrient broth medium) and reduced to adenosine phosphosulphate with the support of the enzyme ATP sulfurylase [40]. The adenosine phosphosulphate further phosphorylated to form 3′phosphoadenosine phosphosulphate. The 3′phosphoadenosine phosphosulphate is reduced to form sulphite ions (SO32−) with the assistance of phosphoadenosine phosphosulphate reductase [39]. Then, the sulphite ions are reduced to sulphide ions (S2−) with the help of sulphite reductase. The reduced sulphide ions are coupled with inorganic metal ions cadmium and zinc in extracellular environment and resulted in the formation of cadmium sulphide and zinc sulphide nanoparticles [40, 41]. Likewise sulphate ions, the selenium ions are also reduced to selenide ions through enzymatic reaction. Previously, Li et al. (2007) have demonstrated the reduction of selenium ions with the assistance of 30 kDa protein molecules present in the extract of Capsicum annuum [42]. The presence of phytochemicals and secondary metabolites in the plant extracts also have the capability to synthesis inorganic nanoparticles [43].

Bottom Line: The results indicated that the proteins, which contain amine groups, played a reducing and controlling responsibility during the formation of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles in the colloidal solution.Staphylococcus sp.Lactobacillus sp., and Candida albicans and these results confirmed that the sulphide nanoparticles are exhibiting good bactericidal activity.

View Article: PubMed Central - PubMed

Affiliation: Environmental Nanotechnology Division, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, Tamilnadu 627412, India.

ABSTRACT
Dental care is an essential phenomenon in human health. Oral pathogens can cause severe break which may show the way to serious issues in human disease like blood circulation and coronary disease. In the current study, we demonstrated the synthesis and antimicrobial activity of cadmium sulphide and zinc sulphide nanoparticles against oral pathogens. The process for the synthesis of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles is fast, novel, and ecofriendly. Formation of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles was confirmed by surface plasmon spectra using UV-Vis spectrophotometer. The morphology of crystalline phase of nanoparticles was determined from transmission electron microscopy (TEM) and X-ray diffraction (XRD) spectra. The average size of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles was in the range of 10 nm to 25 nm and 65 nm, respectively, and the observed morphology was spherical. The results indicated that the proteins, which contain amine groups, played a reducing and controlling responsibility during the formation of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles in the colloidal solution. The antimicrobial activity was assessed against oral pathogens such as Streptococcus sp. Staphylococcus sp. Lactobacillus sp., and Candida albicans and these results confirmed that the sulphide nanoparticles are exhibiting good bactericidal activity.

No MeSH data available.


Related in: MedlinePlus