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Yiqi Huoxue Recipe Improves Heart Function through Inhibiting Apoptosis Related to Endoplasmic Reticulum Stress in Myocardial Infarction Model of Rats.

Lou LX, Wu AM, Zhang DM, Wu SX, Gao YH, Nie B, Zhao MJ, Lv XY, Jin QS, Zhao YZ, Wang SR, Chai LM - Evid Based Complement Alternat Med (2014)

Bottom Line: To determine whether the endoplasmic reticulum (ER) stress response pathway is included in the cardioprotective function of the recipe, ER stress related proteins such as GRP78 and caspase-12 were examined.Results.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China.

ABSTRACT
Objective. To explore the mechanism of cardioprotective effects of Chinese medicine, Yiqi Huoxue recipe, in rats with myocardial infarction- (MI-) induced heart failure. Methods. Male Sprague-Dawley rats underwent left anterior descending artery (LAD) ligation or sham operation. The surviving MI rats were divided randomly into three groups: MI (5 mL/kg/d NS by gavage), MI + Metoprolol Tartrate (MT) (12 mg/kg/d MT by gavage), and MI + Yiqi Huoxue (5 mL/kg recipe by gavage). And the sham operation rats were given 5 mL/kg/d normal saline. Treatments were given on the day following surgery for 4 weeks. Then rats were detected for heart structure and function by transthoracic echocardiography. Apoptosis in heart tissues was detected by TUNEL staining. To determine whether the endoplasmic reticulum (ER) stress response pathway is included in the cardioprotective function of the recipe, ER stress related proteins such as GRP78 and caspase-12 were examined. Results. Yiqi Huoxue recipe attenuated heart function injury, reversed histopathological damage, alleviated myocardial apoptosis and inhibited ER stress in MI rats. Conclusion. All the results suggest that Yiqi Huoxue recipe improves the injured heart function maybe through inhibition of ER stress response pathway, which is a promising target in therapy for heart failure.

No MeSH data available.


Related in: MedlinePlus

Expression of glucose response protein 78 (GRP78) and caspase-12 in mRNA level (a, b) and protein level (c, d) in heart tissues. Rats underwent MI surgery and after 4 weeks of treatment hearts were obtained. Heart tissues extracts were analyzed by immunoblotting by using antibodies against GRP78 and caspase-12. GAPDH was used as a loading control. Data are the mean ± SD (n = 3). *P < 0.05, **P < 0.01 versus sham group. #P < 0.05, ##P < 0.01 versus myocardial infarction group.
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fig4: Expression of glucose response protein 78 (GRP78) and caspase-12 in mRNA level (a, b) and protein level (c, d) in heart tissues. Rats underwent MI surgery and after 4 weeks of treatment hearts were obtained. Heart tissues extracts were analyzed by immunoblotting by using antibodies against GRP78 and caspase-12. GAPDH was used as a loading control. Data are the mean ± SD (n = 3). *P < 0.05, **P < 0.01 versus sham group. #P < 0.05, ##P < 0.01 versus myocardial infarction group.

Mentions: Endoplasmic reticulum stress has been reported as an independent pathway leading to apoptosis and a new mechanism implicated in heart failure [5, 6]. To investigate the possible mechanism of heart protection of Yiqi Huoxue recipe, ERS related proteins such as GRP78 and caspase-12 were examined. Real-time PCR analysis of samples from heart tissue showed that GRP78 and caspase-12 mRNA levels were increased in MI rats hearts compared with the sham group (Figures 4(a) and 4(b)). When rats were treated with Yiqi Huoxue recipe or MT after LAD occlusion operation, GRP78 and caspase-12 mRNA levels in heart tissue were downregulated compared with those of MI rats without treatment (Figures 4(a) and 4(b)). Western blot showed that the protein levels of these two factors were increased in MI heart and Yiqi Huoxue recipe treatment can decrease the protein levels. And as to caspase-12, both the pro- and activated caspase-12 showed the same trend (Figures 4(c) and 4(d)).


Yiqi Huoxue Recipe Improves Heart Function through Inhibiting Apoptosis Related to Endoplasmic Reticulum Stress in Myocardial Infarction Model of Rats.

Lou LX, Wu AM, Zhang DM, Wu SX, Gao YH, Nie B, Zhao MJ, Lv XY, Jin QS, Zhao YZ, Wang SR, Chai LM - Evid Based Complement Alternat Med (2014)

Expression of glucose response protein 78 (GRP78) and caspase-12 in mRNA level (a, b) and protein level (c, d) in heart tissues. Rats underwent MI surgery and after 4 weeks of treatment hearts were obtained. Heart tissues extracts were analyzed by immunoblotting by using antibodies against GRP78 and caspase-12. GAPDH was used as a loading control. Data are the mean ± SD (n = 3). *P < 0.05, **P < 0.01 versus sham group. #P < 0.05, ##P < 0.01 versus myocardial infarction group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4016842&req=5

fig4: Expression of glucose response protein 78 (GRP78) and caspase-12 in mRNA level (a, b) and protein level (c, d) in heart tissues. Rats underwent MI surgery and after 4 weeks of treatment hearts were obtained. Heart tissues extracts were analyzed by immunoblotting by using antibodies against GRP78 and caspase-12. GAPDH was used as a loading control. Data are the mean ± SD (n = 3). *P < 0.05, **P < 0.01 versus sham group. #P < 0.05, ##P < 0.01 versus myocardial infarction group.
Mentions: Endoplasmic reticulum stress has been reported as an independent pathway leading to apoptosis and a new mechanism implicated in heart failure [5, 6]. To investigate the possible mechanism of heart protection of Yiqi Huoxue recipe, ERS related proteins such as GRP78 and caspase-12 were examined. Real-time PCR analysis of samples from heart tissue showed that GRP78 and caspase-12 mRNA levels were increased in MI rats hearts compared with the sham group (Figures 4(a) and 4(b)). When rats were treated with Yiqi Huoxue recipe or MT after LAD occlusion operation, GRP78 and caspase-12 mRNA levels in heart tissue were downregulated compared with those of MI rats without treatment (Figures 4(a) and 4(b)). Western blot showed that the protein levels of these two factors were increased in MI heart and Yiqi Huoxue recipe treatment can decrease the protein levels. And as to caspase-12, both the pro- and activated caspase-12 showed the same trend (Figures 4(c) and 4(d)).

Bottom Line: To determine whether the endoplasmic reticulum (ER) stress response pathway is included in the cardioprotective function of the recipe, ER stress related proteins such as GRP78 and caspase-12 were examined.Results.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China.

ABSTRACT
Objective. To explore the mechanism of cardioprotective effects of Chinese medicine, Yiqi Huoxue recipe, in rats with myocardial infarction- (MI-) induced heart failure. Methods. Male Sprague-Dawley rats underwent left anterior descending artery (LAD) ligation or sham operation. The surviving MI rats were divided randomly into three groups: MI (5 mL/kg/d NS by gavage), MI + Metoprolol Tartrate (MT) (12 mg/kg/d MT by gavage), and MI + Yiqi Huoxue (5 mL/kg recipe by gavage). And the sham operation rats were given 5 mL/kg/d normal saline. Treatments were given on the day following surgery for 4 weeks. Then rats were detected for heart structure and function by transthoracic echocardiography. Apoptosis in heart tissues was detected by TUNEL staining. To determine whether the endoplasmic reticulum (ER) stress response pathway is included in the cardioprotective function of the recipe, ER stress related proteins such as GRP78 and caspase-12 were examined. Results. Yiqi Huoxue recipe attenuated heart function injury, reversed histopathological damage, alleviated myocardial apoptosis and inhibited ER stress in MI rats. Conclusion. All the results suggest that Yiqi Huoxue recipe improves the injured heart function maybe through inhibition of ER stress response pathway, which is a promising target in therapy for heart failure.

No MeSH data available.


Related in: MedlinePlus