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Yiqi Huoxue Recipe Improves Heart Function through Inhibiting Apoptosis Related to Endoplasmic Reticulum Stress in Myocardial Infarction Model of Rats.

Lou LX, Wu AM, Zhang DM, Wu SX, Gao YH, Nie B, Zhao MJ, Lv XY, Jin QS, Zhao YZ, Wang SR, Chai LM - Evid Based Complement Alternat Med (2014)

Bottom Line: To determine whether the endoplasmic reticulum (ER) stress response pathway is included in the cardioprotective function of the recipe, ER stress related proteins such as GRP78 and caspase-12 were examined.Results.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China.

ABSTRACT
Objective. To explore the mechanism of cardioprotective effects of Chinese medicine, Yiqi Huoxue recipe, in rats with myocardial infarction- (MI-) induced heart failure. Methods. Male Sprague-Dawley rats underwent left anterior descending artery (LAD) ligation or sham operation. The surviving MI rats were divided randomly into three groups: MI (5 mL/kg/d NS by gavage), MI + Metoprolol Tartrate (MT) (12 mg/kg/d MT by gavage), and MI + Yiqi Huoxue (5 mL/kg recipe by gavage). And the sham operation rats were given 5 mL/kg/d normal saline. Treatments were given on the day following surgery for 4 weeks. Then rats were detected for heart structure and function by transthoracic echocardiography. Apoptosis in heart tissues was detected by TUNEL staining. To determine whether the endoplasmic reticulum (ER) stress response pathway is included in the cardioprotective function of the recipe, ER stress related proteins such as GRP78 and caspase-12 were examined. Results. Yiqi Huoxue recipe attenuated heart function injury, reversed histopathological damage, alleviated myocardial apoptosis and inhibited ER stress in MI rats. Conclusion. All the results suggest that Yiqi Huoxue recipe improves the injured heart function maybe through inhibition of ER stress response pathway, which is a promising target in therapy for heart failure.

No MeSH data available.


Related in: MedlinePlus

Yiqi Huoxue recipe attenuates heart function injury and attenuates the heart to body weight ratio in myocardial infarcted rats model. Rats were operated with myocardial infarction (MI) surgery and after 4 weeks of treatment, echocardiography assay was performed ((a) sham, (b) MI, (c) MI + Metoprolol Tartrate, (d) MI + Yiqi Huoxue recipe, and (e) anatomic and echocardiographic data for rats 4 weeks after myocardial infarction with different treatment). And after sacrificing the rats, the heart to body weight ratio was also calculated. In the rat myocardial infarction (MI) model, the heart to body weight ratio is increased in the untreated MI group at 4 weeks after MI. Yiqi Huoxue recipe and MT treatment diminished the effect (e). As to the echocardiographic data, the LVEF and LVFS showed the same trend (e). Values are mean ± SD (n = 9), LVEF, left ventricular ejection fraction; LVFS, left ventricular fractional shortening; LVDd, left ventricular end-diastolic volume; LVDs, left ventricular end-systolic volume. *P < 0.05, **P < 0.01 versus sham group. #P < 0.05 versus myocardial infarction group.
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fig1: Yiqi Huoxue recipe attenuates heart function injury and attenuates the heart to body weight ratio in myocardial infarcted rats model. Rats were operated with myocardial infarction (MI) surgery and after 4 weeks of treatment, echocardiography assay was performed ((a) sham, (b) MI, (c) MI + Metoprolol Tartrate, (d) MI + Yiqi Huoxue recipe, and (e) anatomic and echocardiographic data for rats 4 weeks after myocardial infarction with different treatment). And after sacrificing the rats, the heart to body weight ratio was also calculated. In the rat myocardial infarction (MI) model, the heart to body weight ratio is increased in the untreated MI group at 4 weeks after MI. Yiqi Huoxue recipe and MT treatment diminished the effect (e). As to the echocardiographic data, the LVEF and LVFS showed the same trend (e). Values are mean ± SD (n = 9), LVEF, left ventricular ejection fraction; LVFS, left ventricular fractional shortening; LVDd, left ventricular end-diastolic volume; LVDs, left ventricular end-systolic volume. *P < 0.05, **P < 0.01 versus sham group. #P < 0.05 versus myocardial infarction group.

Mentions: To determine the effect of the Yiqi Huoxue recipe on treatment of MI-induced heart failure in vivo, we induced MI in rats and detected the cardiac function by echocardiography assay and after sacrificing the rats we also measured the heart to body weight ratio. The result showed that a significant increase in the volume of left ventricle was demonstrated by the increase of LVDd and LVDs after 4 weeks of infarction (Figure 1(e)), which is attributed to the left ventricular dilatation. However, MT or Yiqi Huoxue recipe treatment had no significant effect on the MI-induced dilation. The result also demonstrated that the LVEF and LVFS were decreased significantly 4 weeks after MI surgery. Treatment with MT or Yiqi Huoxue recipe improved the cardiac function by increase in LVEF by 21% or 16.9% and in LVFS by 50.3% or 40.8%, respectively, compared with MI group (Figure 1(e)). However, MT or Yiqi Huoxue recipe showed no difference in the LVEF and LVFS improvements (P > 0.05). The heart to body weight ratio was increased in MI group rats compared with that in sham group rats (P < 0.05). The treatment with MT or Yiqi Huoxue recipe was observed to attenuate the increase in the heart to body weight ratio at 4 weeks after myocardial infarction surgery (P < 0.05) and showed no difference (P > 0.05) (Figure 1(e)).


Yiqi Huoxue Recipe Improves Heart Function through Inhibiting Apoptosis Related to Endoplasmic Reticulum Stress in Myocardial Infarction Model of Rats.

Lou LX, Wu AM, Zhang DM, Wu SX, Gao YH, Nie B, Zhao MJ, Lv XY, Jin QS, Zhao YZ, Wang SR, Chai LM - Evid Based Complement Alternat Med (2014)

Yiqi Huoxue recipe attenuates heart function injury and attenuates the heart to body weight ratio in myocardial infarcted rats model. Rats were operated with myocardial infarction (MI) surgery and after 4 weeks of treatment, echocardiography assay was performed ((a) sham, (b) MI, (c) MI + Metoprolol Tartrate, (d) MI + Yiqi Huoxue recipe, and (e) anatomic and echocardiographic data for rats 4 weeks after myocardial infarction with different treatment). And after sacrificing the rats, the heart to body weight ratio was also calculated. In the rat myocardial infarction (MI) model, the heart to body weight ratio is increased in the untreated MI group at 4 weeks after MI. Yiqi Huoxue recipe and MT treatment diminished the effect (e). As to the echocardiographic data, the LVEF and LVFS showed the same trend (e). Values are mean ± SD (n = 9), LVEF, left ventricular ejection fraction; LVFS, left ventricular fractional shortening; LVDd, left ventricular end-diastolic volume; LVDs, left ventricular end-systolic volume. *P < 0.05, **P < 0.01 versus sham group. #P < 0.05 versus myocardial infarction group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4016842&req=5

fig1: Yiqi Huoxue recipe attenuates heart function injury and attenuates the heart to body weight ratio in myocardial infarcted rats model. Rats were operated with myocardial infarction (MI) surgery and after 4 weeks of treatment, echocardiography assay was performed ((a) sham, (b) MI, (c) MI + Metoprolol Tartrate, (d) MI + Yiqi Huoxue recipe, and (e) anatomic and echocardiographic data for rats 4 weeks after myocardial infarction with different treatment). And after sacrificing the rats, the heart to body weight ratio was also calculated. In the rat myocardial infarction (MI) model, the heart to body weight ratio is increased in the untreated MI group at 4 weeks after MI. Yiqi Huoxue recipe and MT treatment diminished the effect (e). As to the echocardiographic data, the LVEF and LVFS showed the same trend (e). Values are mean ± SD (n = 9), LVEF, left ventricular ejection fraction; LVFS, left ventricular fractional shortening; LVDd, left ventricular end-diastolic volume; LVDs, left ventricular end-systolic volume. *P < 0.05, **P < 0.01 versus sham group. #P < 0.05 versus myocardial infarction group.
Mentions: To determine the effect of the Yiqi Huoxue recipe on treatment of MI-induced heart failure in vivo, we induced MI in rats and detected the cardiac function by echocardiography assay and after sacrificing the rats we also measured the heart to body weight ratio. The result showed that a significant increase in the volume of left ventricle was demonstrated by the increase of LVDd and LVDs after 4 weeks of infarction (Figure 1(e)), which is attributed to the left ventricular dilatation. However, MT or Yiqi Huoxue recipe treatment had no significant effect on the MI-induced dilation. The result also demonstrated that the LVEF and LVFS were decreased significantly 4 weeks after MI surgery. Treatment with MT or Yiqi Huoxue recipe improved the cardiac function by increase in LVEF by 21% or 16.9% and in LVFS by 50.3% or 40.8%, respectively, compared with MI group (Figure 1(e)). However, MT or Yiqi Huoxue recipe showed no difference in the LVEF and LVFS improvements (P > 0.05). The heart to body weight ratio was increased in MI group rats compared with that in sham group rats (P < 0.05). The treatment with MT or Yiqi Huoxue recipe was observed to attenuate the increase in the heart to body weight ratio at 4 weeks after myocardial infarction surgery (P < 0.05) and showed no difference (P > 0.05) (Figure 1(e)).

Bottom Line: To determine whether the endoplasmic reticulum (ER) stress response pathway is included in the cardioprotective function of the recipe, ER stress related proteins such as GRP78 and caspase-12 were examined.Results.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China.

ABSTRACT
Objective. To explore the mechanism of cardioprotective effects of Chinese medicine, Yiqi Huoxue recipe, in rats with myocardial infarction- (MI-) induced heart failure. Methods. Male Sprague-Dawley rats underwent left anterior descending artery (LAD) ligation or sham operation. The surviving MI rats were divided randomly into three groups: MI (5 mL/kg/d NS by gavage), MI + Metoprolol Tartrate (MT) (12 mg/kg/d MT by gavage), and MI + Yiqi Huoxue (5 mL/kg recipe by gavage). And the sham operation rats were given 5 mL/kg/d normal saline. Treatments were given on the day following surgery for 4 weeks. Then rats were detected for heart structure and function by transthoracic echocardiography. Apoptosis in heart tissues was detected by TUNEL staining. To determine whether the endoplasmic reticulum (ER) stress response pathway is included in the cardioprotective function of the recipe, ER stress related proteins such as GRP78 and caspase-12 were examined. Results. Yiqi Huoxue recipe attenuated heart function injury, reversed histopathological damage, alleviated myocardial apoptosis and inhibited ER stress in MI rats. Conclusion. All the results suggest that Yiqi Huoxue recipe improves the injured heart function maybe through inhibition of ER stress response pathway, which is a promising target in therapy for heart failure.

No MeSH data available.


Related in: MedlinePlus