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Does long-term use of silver nanoparticles have persistent inhibitory effect on H. pylori based on Mongolian gerbil's model?

Kuo CH, Lu CY, Yang YC, Chin C, Weng BC, Liu CJ, Chen YH, Chang LL, Kuo FC, Wu DC, Su HL - Biomed Res Int (2014)

Bottom Line: The AgNP/clay has more obvious inhibitory effect on H. pylori in vitro.There were no significant differences of inflammation among groups D, E, and F (P = 0.688).AgNP/clay would be a potential and safe agent for inhibiting H. pylori.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tz-You 1st Road, Kaohsiung City 807, Taiwan ; Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung City 807, Taiwan.

ABSTRACT

Background: It is urgent to find alternative agents due to increasing failure rate of Helicobacter pylori (H. pylori) eradication. The study surveyed the long-term effect of silver nanoparticles (AgNP) on H. pylori based on Mongolian gerbil's model.

Materials and methods: Fifty gerbils were randomly allocated to six groups (A-F). Group (Gr) A: the gerbils were fed with broth; Gr B and D: the gerbils were fed with AgNP/clay complex (0.1% of weight); Gr C and E: the gerbils were fed with AgNP/clay complex(1% of weight); and Gr D, E, and F: the gerbils were inoculated with H. pylori. At the 20th experimental week, the gerbils were sacrificed. Histology was evaluated according to the classification of the Sydney system. P < 0.05 was considered to be statistically significant.

Results: The AgNP/clay has more obvious inhibitory effect on H. pylori in vitro. There was a trend of higher concentrations of AgNP with stronger inhibitory effect on H. pylori growth (P = 0.071). There were no significant differences of inflammation among groups D, E, and F (P = 0.688).

Conclusion: AgNP/clay would be a potential and safe agent for inhibiting H. pylori. It should be helpful for eradication of H. pylori infection.

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Related in: MedlinePlus

Histological changes of different groups are shown (H&E stain 1000x). We show the status of H. pylori density in right field and the status of inflammation in left field. There is no obvious inflammatory cells infiltration in groups A, B, and C, but obvious inflammatory cells infiltration was noted in groups D, E, and F. The densities of H. pylori were higher in group F.
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fig6: Histological changes of different groups are shown (H&E stain 1000x). We show the status of H. pylori density in right field and the status of inflammation in left field. There is no obvious inflammatory cells infiltration in groups A, B, and C, but obvious inflammatory cells infiltration was noted in groups D, E, and F. The densities of H. pylori were higher in group F.

Mentions: We surveyed the densities of H. pylori in groups D, E, and F. We wanted to survey the inhibitory effect of AgNP on H. pylori. The average densities of H. pylori (according to Sydney classification) were 1.45 ± 0.52, 1.27 ± 0.70, and 1.83 ± 0.41 in Gr D, E, and F, respectively. It did not show significant difference between the three groups (P = 0.071); however, we found the trend that higher concentrations of AgNP had stronger effect on inhibiting H. pylori (Figures 4 and 6).


Does long-term use of silver nanoparticles have persistent inhibitory effect on H. pylori based on Mongolian gerbil's model?

Kuo CH, Lu CY, Yang YC, Chin C, Weng BC, Liu CJ, Chen YH, Chang LL, Kuo FC, Wu DC, Su HL - Biomed Res Int (2014)

Histological changes of different groups are shown (H&E stain 1000x). We show the status of H. pylori density in right field and the status of inflammation in left field. There is no obvious inflammatory cells infiltration in groups A, B, and C, but obvious inflammatory cells infiltration was noted in groups D, E, and F. The densities of H. pylori were higher in group F.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016839&req=5

fig6: Histological changes of different groups are shown (H&E stain 1000x). We show the status of H. pylori density in right field and the status of inflammation in left field. There is no obvious inflammatory cells infiltration in groups A, B, and C, but obvious inflammatory cells infiltration was noted in groups D, E, and F. The densities of H. pylori were higher in group F.
Mentions: We surveyed the densities of H. pylori in groups D, E, and F. We wanted to survey the inhibitory effect of AgNP on H. pylori. The average densities of H. pylori (according to Sydney classification) were 1.45 ± 0.52, 1.27 ± 0.70, and 1.83 ± 0.41 in Gr D, E, and F, respectively. It did not show significant difference between the three groups (P = 0.071); however, we found the trend that higher concentrations of AgNP had stronger effect on inhibiting H. pylori (Figures 4 and 6).

Bottom Line: The AgNP/clay has more obvious inhibitory effect on H. pylori in vitro.There were no significant differences of inflammation among groups D, E, and F (P = 0.688).AgNP/clay would be a potential and safe agent for inhibiting H. pylori.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tz-You 1st Road, Kaohsiung City 807, Taiwan ; Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung City 807, Taiwan.

ABSTRACT

Background: It is urgent to find alternative agents due to increasing failure rate of Helicobacter pylori (H. pylori) eradication. The study surveyed the long-term effect of silver nanoparticles (AgNP) on H. pylori based on Mongolian gerbil's model.

Materials and methods: Fifty gerbils were randomly allocated to six groups (A-F). Group (Gr) A: the gerbils were fed with broth; Gr B and D: the gerbils were fed with AgNP/clay complex (0.1% of weight); Gr C and E: the gerbils were fed with AgNP/clay complex(1% of weight); and Gr D, E, and F: the gerbils were inoculated with H. pylori. At the 20th experimental week, the gerbils were sacrificed. Histology was evaluated according to the classification of the Sydney system. P < 0.05 was considered to be statistically significant.

Results: The AgNP/clay has more obvious inhibitory effect on H. pylori in vitro. There was a trend of higher concentrations of AgNP with stronger inhibitory effect on H. pylori growth (P = 0.071). There were no significant differences of inflammation among groups D, E, and F (P = 0.688).

Conclusion: AgNP/clay would be a potential and safe agent for inhibiting H. pylori. It should be helpful for eradication of H. pylori infection.

Show MeSH
Related in: MedlinePlus