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Does long-term use of silver nanoparticles have persistent inhibitory effect on H. pylori based on Mongolian gerbil's model?

Kuo CH, Lu CY, Yang YC, Chin C, Weng BC, Liu CJ, Chen YH, Chang LL, Kuo FC, Wu DC, Su HL - Biomed Res Int (2014)

Bottom Line: The AgNP/clay has more obvious inhibitory effect on H. pylori in vitro.There were no significant differences of inflammation among groups D, E, and F (P = 0.688).AgNP/clay would be a potential and safe agent for inhibiting H. pylori.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tz-You 1st Road, Kaohsiung City 807, Taiwan ; Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung City 807, Taiwan.

ABSTRACT

Background: It is urgent to find alternative agents due to increasing failure rate of Helicobacter pylori (H. pylori) eradication. The study surveyed the long-term effect of silver nanoparticles (AgNP) on H. pylori based on Mongolian gerbil's model.

Materials and methods: Fifty gerbils were randomly allocated to six groups (A-F). Group (Gr) A: the gerbils were fed with broth; Gr B and D: the gerbils were fed with AgNP/clay complex (0.1% of weight); Gr C and E: the gerbils were fed with AgNP/clay complex(1% of weight); and Gr D, E, and F: the gerbils were inoculated with H. pylori. At the 20th experimental week, the gerbils were sacrificed. Histology was evaluated according to the classification of the Sydney system. P < 0.05 was considered to be statistically significant.

Results: The AgNP/clay has more obvious inhibitory effect on H. pylori in vitro. There was a trend of higher concentrations of AgNP with stronger inhibitory effect on H. pylori growth (P = 0.071). There were no significant differences of inflammation among groups D, E, and F (P = 0.688).

Conclusion: AgNP/clay would be a potential and safe agent for inhibiting H. pylori. It should be helpful for eradication of H. pylori infection.

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Related in: MedlinePlus

The inflammatory severities of gerbils' stomach in different groups are shown. All gerbils in groups A, B, and C showed mild inflammation only. The proportion of obvious inflammation was more than mild inflammation in groups D, E, and F. The difference was the most obvious in group F. However, there was no significant difference among these groups.
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fig5: The inflammatory severities of gerbils' stomach in different groups are shown. All gerbils in groups A, B, and C showed mild inflammation only. The proportion of obvious inflammation was more than mild inflammation in groups D, E, and F. The difference was the most obvious in group F. However, there was no significant difference among these groups.

Mentions: The possible toxic effect of AgNP on gastric mucosa and its interaction with H. pylori were also surveyed. We showed the severity of inflammation of gerbil's mucosa according to the Sydney classification. There was no sign of inflammation noted in group A. However, all gerbils in groups B and C had mild monocyte infiltration but without neutrophil infiltration. The results of the inflammatory scores were 0 ± 0 (Gr A), 1.00 ± 0 (Gr B), 1.00 ± 0 (Gr C), 4.27 ± 1.10 (Gr D), 4.20 ± 1.82 (Gr E), and 4.83 ± 1.17 (Gr F). The proportions of moderate to severe inflammation were 0% (0/6), 0% (0/6), 0% (0/6), 54.5% (6/11), 53.3% (8/15), and 66.7% (4/6) in groups A, B, C, D, E, and F (Figures 5 and 6). The results revealed that AgNP alone did not have an acute toxic effect on gerbil's gastric mucosa. Besides these, there were no significant differences of inflammation among groups D, E, and F (P = 0.688).


Does long-term use of silver nanoparticles have persistent inhibitory effect on H. pylori based on Mongolian gerbil's model?

Kuo CH, Lu CY, Yang YC, Chin C, Weng BC, Liu CJ, Chen YH, Chang LL, Kuo FC, Wu DC, Su HL - Biomed Res Int (2014)

The inflammatory severities of gerbils' stomach in different groups are shown. All gerbils in groups A, B, and C showed mild inflammation only. The proportion of obvious inflammation was more than mild inflammation in groups D, E, and F. The difference was the most obvious in group F. However, there was no significant difference among these groups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016839&req=5

fig5: The inflammatory severities of gerbils' stomach in different groups are shown. All gerbils in groups A, B, and C showed mild inflammation only. The proportion of obvious inflammation was more than mild inflammation in groups D, E, and F. The difference was the most obvious in group F. However, there was no significant difference among these groups.
Mentions: The possible toxic effect of AgNP on gastric mucosa and its interaction with H. pylori were also surveyed. We showed the severity of inflammation of gerbil's mucosa according to the Sydney classification. There was no sign of inflammation noted in group A. However, all gerbils in groups B and C had mild monocyte infiltration but without neutrophil infiltration. The results of the inflammatory scores were 0 ± 0 (Gr A), 1.00 ± 0 (Gr B), 1.00 ± 0 (Gr C), 4.27 ± 1.10 (Gr D), 4.20 ± 1.82 (Gr E), and 4.83 ± 1.17 (Gr F). The proportions of moderate to severe inflammation were 0% (0/6), 0% (0/6), 0% (0/6), 54.5% (6/11), 53.3% (8/15), and 66.7% (4/6) in groups A, B, C, D, E, and F (Figures 5 and 6). The results revealed that AgNP alone did not have an acute toxic effect on gerbil's gastric mucosa. Besides these, there were no significant differences of inflammation among groups D, E, and F (P = 0.688).

Bottom Line: The AgNP/clay has more obvious inhibitory effect on H. pylori in vitro.There were no significant differences of inflammation among groups D, E, and F (P = 0.688).AgNP/clay would be a potential and safe agent for inhibiting H. pylori.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tz-You 1st Road, Kaohsiung City 807, Taiwan ; Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung City 807, Taiwan.

ABSTRACT

Background: It is urgent to find alternative agents due to increasing failure rate of Helicobacter pylori (H. pylori) eradication. The study surveyed the long-term effect of silver nanoparticles (AgNP) on H. pylori based on Mongolian gerbil's model.

Materials and methods: Fifty gerbils were randomly allocated to six groups (A-F). Group (Gr) A: the gerbils were fed with broth; Gr B and D: the gerbils were fed with AgNP/clay complex (0.1% of weight); Gr C and E: the gerbils were fed with AgNP/clay complex(1% of weight); and Gr D, E, and F: the gerbils were inoculated with H. pylori. At the 20th experimental week, the gerbils were sacrificed. Histology was evaluated according to the classification of the Sydney system. P < 0.05 was considered to be statistically significant.

Results: The AgNP/clay has more obvious inhibitory effect on H. pylori in vitro. There was a trend of higher concentrations of AgNP with stronger inhibitory effect on H. pylori growth (P = 0.071). There were no significant differences of inflammation among groups D, E, and F (P = 0.688).

Conclusion: AgNP/clay would be a potential and safe agent for inhibiting H. pylori. It should be helpful for eradication of H. pylori infection.

Show MeSH
Related in: MedlinePlus