Limits...
Does long-term use of silver nanoparticles have persistent inhibitory effect on H. pylori based on Mongolian gerbil's model?

Kuo CH, Lu CY, Yang YC, Chin C, Weng BC, Liu CJ, Chen YH, Chang LL, Kuo FC, Wu DC, Su HL - Biomed Res Int (2014)

Bottom Line: The AgNP/clay has more obvious inhibitory effect on H. pylori in vitro.There were no significant differences of inflammation among groups D, E, and F (P = 0.688).AgNP/clay would be a potential and safe agent for inhibiting H. pylori.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tz-You 1st Road, Kaohsiung City 807, Taiwan ; Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung City 807, Taiwan.

ABSTRACT

Background: It is urgent to find alternative agents due to increasing failure rate of Helicobacter pylori (H. pylori) eradication. The study surveyed the long-term effect of silver nanoparticles (AgNP) on H. pylori based on Mongolian gerbil's model.

Materials and methods: Fifty gerbils were randomly allocated to six groups (A-F). Group (Gr) A: the gerbils were fed with broth; Gr B and D: the gerbils were fed with AgNP/clay complex (0.1% of weight); Gr C and E: the gerbils were fed with AgNP/clay complex(1% of weight); and Gr D, E, and F: the gerbils were inoculated with H. pylori. At the 20th experimental week, the gerbils were sacrificed. Histology was evaluated according to the classification of the Sydney system. P < 0.05 was considered to be statistically significant.

Results: The AgNP/clay has more obvious inhibitory effect on H. pylori in vitro. There was a trend of higher concentrations of AgNP with stronger inhibitory effect on H. pylori growth (P = 0.071). There were no significant differences of inflammation among groups D, E, and F (P = 0.688).

Conclusion: AgNP/clay would be a potential and safe agent for inhibiting H. pylori. It should be helpful for eradication of H. pylori infection.

Show MeSH

Related in: MedlinePlus

The timing of AgNP/clay and H. pylori given. Group (Gr) A: the gerbils were fed with broth only. Gr B and D: the gerbils were fed with AgNP/clay (0.1% of weight) in the 8th to 20th week. Gr C and E: the gerbils were fed with AgNP/clay (1% of weight) in the 8th to 20th week. Gr D, E, and F: the gerbils were inoculated with H. pylori [CagA (+)/VacA (+)] in the 1st to 4th week. At the end of the 20th experimental week, the animals were fasted for 24 hours before being sacrificed.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4016839&req=5

fig1: The timing of AgNP/clay and H. pylori given. Group (Gr) A: the gerbils were fed with broth only. Gr B and D: the gerbils were fed with AgNP/clay (0.1% of weight) in the 8th to 20th week. Gr C and E: the gerbils were fed with AgNP/clay (1% of weight) in the 8th to 20th week. Gr D, E, and F: the gerbils were inoculated with H. pylori [CagA (+)/VacA (+)] in the 1st to 4th week. At the end of the 20th experimental week, the animals were fasted for 24 hours before being sacrificed.

Mentions: The gerbils were randomly allocated to six groups according to a randomized number (A–F): group A: the gerbils were fed with broth only; group B: the gerbils were fed with AgNP/clay complex (0.1% of weight) in the 8th to 20th week; group C: the gerbils were fed with AgNP/clay complex (1% of weight) in the 8th to 20th week; group D: the gerbils were inoculated with H. pylori [CagA (+)/VacA (+)] during the 1st to 4th week and then they were fed with AgNP/clay complex (0.1% of weight) in the 8th to 20th week; group E: the gerbils were inoculated with H. pylori [CagA (+)/VacA (+)] during the 1st to 4th week and then they were fed with AgNP/clay complex (1% of weight) in the 8th to 20th week; group F: the gerbils were inoculated with H. pylori [CagA (+)/VacA (+)] during the 1st to 4th week. At the end of the 20th experimental week, the animals were fasted for 24 hours before being sacrificed (Figure 1).


Does long-term use of silver nanoparticles have persistent inhibitory effect on H. pylori based on Mongolian gerbil's model?

Kuo CH, Lu CY, Yang YC, Chin C, Weng BC, Liu CJ, Chen YH, Chang LL, Kuo FC, Wu DC, Su HL - Biomed Res Int (2014)

The timing of AgNP/clay and H. pylori given. Group (Gr) A: the gerbils were fed with broth only. Gr B and D: the gerbils were fed with AgNP/clay (0.1% of weight) in the 8th to 20th week. Gr C and E: the gerbils were fed with AgNP/clay (1% of weight) in the 8th to 20th week. Gr D, E, and F: the gerbils were inoculated with H. pylori [CagA (+)/VacA (+)] in the 1st to 4th week. At the end of the 20th experimental week, the animals were fasted for 24 hours before being sacrificed.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016839&req=5

fig1: The timing of AgNP/clay and H. pylori given. Group (Gr) A: the gerbils were fed with broth only. Gr B and D: the gerbils were fed with AgNP/clay (0.1% of weight) in the 8th to 20th week. Gr C and E: the gerbils were fed with AgNP/clay (1% of weight) in the 8th to 20th week. Gr D, E, and F: the gerbils were inoculated with H. pylori [CagA (+)/VacA (+)] in the 1st to 4th week. At the end of the 20th experimental week, the animals were fasted for 24 hours before being sacrificed.
Mentions: The gerbils were randomly allocated to six groups according to a randomized number (A–F): group A: the gerbils were fed with broth only; group B: the gerbils were fed with AgNP/clay complex (0.1% of weight) in the 8th to 20th week; group C: the gerbils were fed with AgNP/clay complex (1% of weight) in the 8th to 20th week; group D: the gerbils were inoculated with H. pylori [CagA (+)/VacA (+)] during the 1st to 4th week and then they were fed with AgNP/clay complex (0.1% of weight) in the 8th to 20th week; group E: the gerbils were inoculated with H. pylori [CagA (+)/VacA (+)] during the 1st to 4th week and then they were fed with AgNP/clay complex (1% of weight) in the 8th to 20th week; group F: the gerbils were inoculated with H. pylori [CagA (+)/VacA (+)] during the 1st to 4th week. At the end of the 20th experimental week, the animals were fasted for 24 hours before being sacrificed (Figure 1).

Bottom Line: The AgNP/clay has more obvious inhibitory effect on H. pylori in vitro.There were no significant differences of inflammation among groups D, E, and F (P = 0.688).AgNP/clay would be a potential and safe agent for inhibiting H. pylori.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tz-You 1st Road, Kaohsiung City 807, Taiwan ; Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung City 807, Taiwan.

ABSTRACT

Background: It is urgent to find alternative agents due to increasing failure rate of Helicobacter pylori (H. pylori) eradication. The study surveyed the long-term effect of silver nanoparticles (AgNP) on H. pylori based on Mongolian gerbil's model.

Materials and methods: Fifty gerbils were randomly allocated to six groups (A-F). Group (Gr) A: the gerbils were fed with broth; Gr B and D: the gerbils were fed with AgNP/clay complex (0.1% of weight); Gr C and E: the gerbils were fed with AgNP/clay complex(1% of weight); and Gr D, E, and F: the gerbils were inoculated with H. pylori. At the 20th experimental week, the gerbils were sacrificed. Histology was evaluated according to the classification of the Sydney system. P < 0.05 was considered to be statistically significant.

Results: The AgNP/clay has more obvious inhibitory effect on H. pylori in vitro. There was a trend of higher concentrations of AgNP with stronger inhibitory effect on H. pylori growth (P = 0.071). There were no significant differences of inflammation among groups D, E, and F (P = 0.688).

Conclusion: AgNP/clay would be a potential and safe agent for inhibiting H. pylori. It should be helpful for eradication of H. pylori infection.

Show MeSH
Related in: MedlinePlus