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Extracellular matrix protein 1 is correlated to carcinogenesis and lymphatic metastasis of human gastric cancer.

Wu Q, Li X, Yang H, Lu C, You J, Zhang Z - World J Surg Oncol (2014)

Bottom Line: ECM1 mRNA in tumor specimen was also dramatically amplified.Elevated LMVD and ECM1 were positively correlated (P<0.01).In addition, ECM1 protein expression was also closely associated with depth of tumor invasion and TNM stage (P<0.05, respectively).

View Article: PubMed Central - HTML - PubMed

Affiliation: Xiamen Cancer Centre, the First Affiliated Hospital of Xiamen University (the Teaching Hospital of Fujian Medical University), Xiamen, China. youjunxm@163.com.

ABSTRACT

Background: Tumor-induced lymphangiogenesis is a crucial step in malignant invasion and metastasis. Extracellular matrix protein 1 (ECM1) was recently reported to play a role in lymphangiogenesis. In the present work, we aimed to evaluate the role of ECM1 in gastric cancer and examined whether aberrant expression of ECM1 increased the tumorigenic and metastatic potential of human gastric cancer.

Methods: The mRNA and protein expression of ECM1 in gastric cancer specimen and the noncancerous counterparts from 77 patients were detected by real-time PCR and immunohistochemistry staining. Lymphatic microvessel density (LMVD) in the corresponding serial sections was assessed by counting the lymphatic microvessels labelled by D2-40. The correlations between ECM1 expression, LMVD, and the clinicopathological parameters were examined.

Results: ECM1 protein expression was detected in 70.1% (54/77) of gastric cancer specimen, significantly higher than that in the corresponding counterparts (P<0.01). ECM1 mRNA in tumor specimen was also dramatically amplified. Elevated LMVD and ECM1 were positively correlated (P<0.01). In addition, ECM1 protein expression was also closely associated with depth of tumor invasion and TNM stage (P<0.05, respectively).

Conclusions: ECM1 expression is aberrant elevated in tumor specimen and is closely related to the tumorigenic and metastatic potential of human gastric cancer. Thus, carrying out the protein examination may be beneficial to predict carcinogenesis and metastatic spread of human gastric cancer.

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Comparison of LMVD in tissues without/with lymph node metastasis. (A) LMVD in tumor specimen with lymph node metastasis (LN+) was higher than that without lymph node metastasis (LN-), although the difference did not reach statistical significance (P >0.05). (B) LMVD in non-cancerous gastric mucosa from patients with lymph node metastasis (LN+) was statistically higher than that without metastasis (LN-) (** P <0.01).
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Figure 2: Comparison of LMVD in tissues without/with lymph node metastasis. (A) LMVD in tumor specimen with lymph node metastasis (LN+) was higher than that without lymph node metastasis (LN-), although the difference did not reach statistical significance (P >0.05). (B) LMVD in non-cancerous gastric mucosa from patients with lymph node metastasis (LN+) was statistically higher than that without metastasis (LN-) (** P <0.01).

Mentions: As shown in Table 1, there was no statistical difference of LMVD among groups of different gender, age at diagnosis, differentiation degree, and depth of tumor invasion, lymph node metastasis or TNM stage (P >0.05, respectively). In addition, though the difference was not statistically significant (Mann–Whitney test, P = 0.065), LMVD in the tumor specimen with lymphatic metastasis (9.02 ± 1.16 LMV per × 200 field) tended to be higher than that without lymph node metastasis (6.74 ± 1.29 LMV per × 200 field) (Figure 2A). And LMVD in paracancerous gastric mucosa with lymph node metastasis (3.66 ± 0.70 LMV per × 200 field) was statistically higher than that without metastasis (1.79 ± 0.34 LMV per × 200 field; Mann–Whitney test, P = 0.008) (Figure 2B).


Extracellular matrix protein 1 is correlated to carcinogenesis and lymphatic metastasis of human gastric cancer.

Wu Q, Li X, Yang H, Lu C, You J, Zhang Z - World J Surg Oncol (2014)

Comparison of LMVD in tissues without/with lymph node metastasis. (A) LMVD in tumor specimen with lymph node metastasis (LN+) was higher than that without lymph node metastasis (LN-), although the difference did not reach statistical significance (P >0.05). (B) LMVD in non-cancerous gastric mucosa from patients with lymph node metastasis (LN+) was statistically higher than that without metastasis (LN-) (** P <0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4016775&req=5

Figure 2: Comparison of LMVD in tissues without/with lymph node metastasis. (A) LMVD in tumor specimen with lymph node metastasis (LN+) was higher than that without lymph node metastasis (LN-), although the difference did not reach statistical significance (P >0.05). (B) LMVD in non-cancerous gastric mucosa from patients with lymph node metastasis (LN+) was statistically higher than that without metastasis (LN-) (** P <0.01).
Mentions: As shown in Table 1, there was no statistical difference of LMVD among groups of different gender, age at diagnosis, differentiation degree, and depth of tumor invasion, lymph node metastasis or TNM stage (P >0.05, respectively). In addition, though the difference was not statistically significant (Mann–Whitney test, P = 0.065), LMVD in the tumor specimen with lymphatic metastasis (9.02 ± 1.16 LMV per × 200 field) tended to be higher than that without lymph node metastasis (6.74 ± 1.29 LMV per × 200 field) (Figure 2A). And LMVD in paracancerous gastric mucosa with lymph node metastasis (3.66 ± 0.70 LMV per × 200 field) was statistically higher than that without metastasis (1.79 ± 0.34 LMV per × 200 field; Mann–Whitney test, P = 0.008) (Figure 2B).

Bottom Line: ECM1 mRNA in tumor specimen was also dramatically amplified.Elevated LMVD and ECM1 were positively correlated (P<0.01).In addition, ECM1 protein expression was also closely associated with depth of tumor invasion and TNM stage (P<0.05, respectively).

View Article: PubMed Central - HTML - PubMed

Affiliation: Xiamen Cancer Centre, the First Affiliated Hospital of Xiamen University (the Teaching Hospital of Fujian Medical University), Xiamen, China. youjunxm@163.com.

ABSTRACT

Background: Tumor-induced lymphangiogenesis is a crucial step in malignant invasion and metastasis. Extracellular matrix protein 1 (ECM1) was recently reported to play a role in lymphangiogenesis. In the present work, we aimed to evaluate the role of ECM1 in gastric cancer and examined whether aberrant expression of ECM1 increased the tumorigenic and metastatic potential of human gastric cancer.

Methods: The mRNA and protein expression of ECM1 in gastric cancer specimen and the noncancerous counterparts from 77 patients were detected by real-time PCR and immunohistochemistry staining. Lymphatic microvessel density (LMVD) in the corresponding serial sections was assessed by counting the lymphatic microvessels labelled by D2-40. The correlations between ECM1 expression, LMVD, and the clinicopathological parameters were examined.

Results: ECM1 protein expression was detected in 70.1% (54/77) of gastric cancer specimen, significantly higher than that in the corresponding counterparts (P<0.01). ECM1 mRNA in tumor specimen was also dramatically amplified. Elevated LMVD and ECM1 were positively correlated (P<0.01). In addition, ECM1 protein expression was also closely associated with depth of tumor invasion and TNM stage (P<0.05, respectively).

Conclusions: ECM1 expression is aberrant elevated in tumor specimen and is closely related to the tumorigenic and metastatic potential of human gastric cancer. Thus, carrying out the protein examination may be beneficial to predict carcinogenesis and metastatic spread of human gastric cancer.

Show MeSH
Related in: MedlinePlus