Investigation of sickle-cell haemoglobin polymerisation under electrochemical control.
Bottom Line: The polymerisation was monitored as a change in turbidity during the depletion of oxygen in a small volume custom-built thin-layer electrochemical cell.The cell allowed the investigation of HbS polymerisation as a function of HbS concentration, temperature and solution pH.We confirm that the oxygen was efficiently depleted using finite-element modelling to accurately recreate the electrochemical thin-layer cell.
Affiliation: Department of Chemistry, University College London, 20 Gordon St., London, WC1 H 0AJ, UK.Show MeSH
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Mentions: A series of experiments were performed to investigate HbS polymerisation in the presence of two chemical agents known to interrupt the protein polymerisation. Vanillin (2,4-dihydroxybenzaldehyde) and 5-hydroxymethyl-2-furfural (5HMF) were added to the electrochemical cell at different concentrations and essentially the same turbidity time experiment was carried out to measure the protein concentration when the oxygen was depleted. The chemical agent in both cases was incubated in the protein solution 5 min prior to adding the solution to the cell and commencing the experiment. Figures 5 A,B show unambiguously that in the presence of a high concentration of vanillin and 5HTC the extent of polymerisation was reduced dramatically. Cyclic voltammetry in the region of −0.55 V vs Ag/AgCl showed that both vanillin and 5HMF did not have any electrochemical activity.
Affiliation: Department of Chemistry, University College London, 20 Gordon St., London, WC1 H 0AJ, UK.