Investigation of sickle-cell haemoglobin polymerisation under electrochemical control.
Bottom Line: The polymerisation was monitored as a change in turbidity during the depletion of oxygen in a small volume custom-built thin-layer electrochemical cell.The cell allowed the investigation of HbS polymerisation as a function of HbS concentration, temperature and solution pH.We confirm that the oxygen was efficiently depleted using finite-element modelling to accurately recreate the electrochemical thin-layer cell.
Affiliation: Department of Chemistry, University College London, 20 Gordon St., London, WC1 H 0AJ, UK.Show MeSH
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Mentions: The effect of increasing HbS protein concentration on turbidity, which is an indication of protein aggregation, at a Pt surface was investigated by performing experiments at protein concentrations between 20 mg cm−3 and 100 mg cm−3 at 38 °C. Figure 2 shows the effect of protein concentration on the turbidity change at a wavelength of 700 nm (all other wavelengths showed a similar trend), including 100 mg cm−3 of HbA as a control experiment.
Affiliation: Department of Chemistry, University College London, 20 Gordon St., London, WC1 H 0AJ, UK.