Limits...
Developmental mapping of small-conductance calcium-activated potassium channel expression in the rat nervous system.

Gymnopoulos M, Cingolani LA, Pedarzani P, Stocker M - J. Comp. Neurol. (2014)

Bottom Line: The three SK channel subunits display different developmental expression gradients in distinct CNS regions, with time points of expression and up- or downregulation that can be associated with a range of diverse developmental events.Their early expression in embryonic development suggests an involvement of SK channels in the regulation of developmental processes.Additionally, this study shows how the postnatal ontogenetic patterns lead to the adult expression map for each SK channel subunit and how their coexpression in the same regions or neurons varies throughout development.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology of Neuronal Signals, Max Planck Institute for Experimental Medicine, 37075, Göttingen, Germany.

Show MeSH
Differential expression of SK1 (A–D), SK2 (E–H), and SK3 (I–L) in the embryonic forebrain and cerebral cortex at stages E15 (A,B,E,F,I,J) and E17 (C,D,G,H,K,L). The darkfield photomicrographs show that, at E15, SK2 is strongly expressed in the primordial plexiform layer (PPL; E,F), together with a weaker expression of SK1 (A,B), and in the cortical neuroepithelium (cx). SK3 shows a more diffuse expression throughout the differentiating cortex (I,J). Strong expression of SK1 is visible in the striatal differentiating field (Str), with a weaker one in the adjacent striatal neuroepithelium lining the lateral ventricle (A). SK2 is strongly expressed in both striatal neuroepithelium and differentiating field (E). By contrast, SK3 is hardly detectable in the striatum but is expressed at weak to moderate levels in the adjacent differentiating amygdala (Amg; I). At E17, all three channels are present in the subiculum (S) and hippocampal primordium (Hi; C,G,K). Cortical expression of the three SK channel transcripts at E17 is shown in D,H,L, with SK2 displaying maximal expression levels in the cortical plate (CP; H). For details on the distribution see Tables3. For abbreviations see list. Scale bars = 250 μm in I (applies to A,E,I); 100 μm in J (applies to B,F,J); 250 μm in K (applies to C,G,K); 100 μm in L (applies to D,H,L).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4016743&req=5

fig08: Differential expression of SK1 (A–D), SK2 (E–H), and SK3 (I–L) in the embryonic forebrain and cerebral cortex at stages E15 (A,B,E,F,I,J) and E17 (C,D,G,H,K,L). The darkfield photomicrographs show that, at E15, SK2 is strongly expressed in the primordial plexiform layer (PPL; E,F), together with a weaker expression of SK1 (A,B), and in the cortical neuroepithelium (cx). SK3 shows a more diffuse expression throughout the differentiating cortex (I,J). Strong expression of SK1 is visible in the striatal differentiating field (Str), with a weaker one in the adjacent striatal neuroepithelium lining the lateral ventricle (A). SK2 is strongly expressed in both striatal neuroepithelium and differentiating field (E). By contrast, SK3 is hardly detectable in the striatum but is expressed at weak to moderate levels in the adjacent differentiating amygdala (Amg; I). At E17, all three channels are present in the subiculum (S) and hippocampal primordium (Hi; C,G,K). Cortical expression of the three SK channel transcripts at E17 is shown in D,H,L, with SK2 displaying maximal expression levels in the cortical plate (CP; H). For details on the distribution see Tables3. For abbreviations see list. Scale bars = 250 μm in I (applies to A,E,I); 100 μm in J (applies to B,F,J); 250 μm in K (applies to C,G,K); 100 μm in L (applies to D,H,L).

Mentions: The results of the Northern analysis raised the question of whether the developmental changes in the SK expression levels reflect changes in their distribution patterns before birth. To address this question, we performed a detailed in situ hybridization analysis and mapped the distributions of SK1, SK2, and SK3 mRNAs in the rat embryonic nervous system. An overview of the prenatal distribution of the SK channel subunits was obtained by examination of X-ray film images (Figs. 2, 3), and, for cellular resolution, emulsion-coated sections were analyzed (Figs. 4, 8, 9, 11, 3, Tables3). Overview pictures of whole embryos (E11–E17; Fig. 2) and embryonic heads (E19–E21; Fig. 3) were obtained after hybridization with radioactively labeled oligonucleotides specific for each of the three SK channel subunits. Criteria for strong vs. weak labeling of brain structures were the number of silver grains accumulated above cell somata relative to the strongest hybridization signal for each given oligonucleotide (see also Fig. 3J–L).


Developmental mapping of small-conductance calcium-activated potassium channel expression in the rat nervous system.

Gymnopoulos M, Cingolani LA, Pedarzani P, Stocker M - J. Comp. Neurol. (2014)

Differential expression of SK1 (A–D), SK2 (E–H), and SK3 (I–L) in the embryonic forebrain and cerebral cortex at stages E15 (A,B,E,F,I,J) and E17 (C,D,G,H,K,L). The darkfield photomicrographs show that, at E15, SK2 is strongly expressed in the primordial plexiform layer (PPL; E,F), together with a weaker expression of SK1 (A,B), and in the cortical neuroepithelium (cx). SK3 shows a more diffuse expression throughout the differentiating cortex (I,J). Strong expression of SK1 is visible in the striatal differentiating field (Str), with a weaker one in the adjacent striatal neuroepithelium lining the lateral ventricle (A). SK2 is strongly expressed in both striatal neuroepithelium and differentiating field (E). By contrast, SK3 is hardly detectable in the striatum but is expressed at weak to moderate levels in the adjacent differentiating amygdala (Amg; I). At E17, all three channels are present in the subiculum (S) and hippocampal primordium (Hi; C,G,K). Cortical expression of the three SK channel transcripts at E17 is shown in D,H,L, with SK2 displaying maximal expression levels in the cortical plate (CP; H). For details on the distribution see Tables3. For abbreviations see list. Scale bars = 250 μm in I (applies to A,E,I); 100 μm in J (applies to B,F,J); 250 μm in K (applies to C,G,K); 100 μm in L (applies to D,H,L).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016743&req=5

fig08: Differential expression of SK1 (A–D), SK2 (E–H), and SK3 (I–L) in the embryonic forebrain and cerebral cortex at stages E15 (A,B,E,F,I,J) and E17 (C,D,G,H,K,L). The darkfield photomicrographs show that, at E15, SK2 is strongly expressed in the primordial plexiform layer (PPL; E,F), together with a weaker expression of SK1 (A,B), and in the cortical neuroepithelium (cx). SK3 shows a more diffuse expression throughout the differentiating cortex (I,J). Strong expression of SK1 is visible in the striatal differentiating field (Str), with a weaker one in the adjacent striatal neuroepithelium lining the lateral ventricle (A). SK2 is strongly expressed in both striatal neuroepithelium and differentiating field (E). By contrast, SK3 is hardly detectable in the striatum but is expressed at weak to moderate levels in the adjacent differentiating amygdala (Amg; I). At E17, all three channels are present in the subiculum (S) and hippocampal primordium (Hi; C,G,K). Cortical expression of the three SK channel transcripts at E17 is shown in D,H,L, with SK2 displaying maximal expression levels in the cortical plate (CP; H). For details on the distribution see Tables3. For abbreviations see list. Scale bars = 250 μm in I (applies to A,E,I); 100 μm in J (applies to B,F,J); 250 μm in K (applies to C,G,K); 100 μm in L (applies to D,H,L).
Mentions: The results of the Northern analysis raised the question of whether the developmental changes in the SK expression levels reflect changes in their distribution patterns before birth. To address this question, we performed a detailed in situ hybridization analysis and mapped the distributions of SK1, SK2, and SK3 mRNAs in the rat embryonic nervous system. An overview of the prenatal distribution of the SK channel subunits was obtained by examination of X-ray film images (Figs. 2, 3), and, for cellular resolution, emulsion-coated sections were analyzed (Figs. 4, 8, 9, 11, 3, Tables3). Overview pictures of whole embryos (E11–E17; Fig. 2) and embryonic heads (E19–E21; Fig. 3) were obtained after hybridization with radioactively labeled oligonucleotides specific for each of the three SK channel subunits. Criteria for strong vs. weak labeling of brain structures were the number of silver grains accumulated above cell somata relative to the strongest hybridization signal for each given oligonucleotide (see also Fig. 3J–L).

Bottom Line: The three SK channel subunits display different developmental expression gradients in distinct CNS regions, with time points of expression and up- or downregulation that can be associated with a range of diverse developmental events.Their early expression in embryonic development suggests an involvement of SK channels in the regulation of developmental processes.Additionally, this study shows how the postnatal ontogenetic patterns lead to the adult expression map for each SK channel subunit and how their coexpression in the same regions or neurons varies throughout development.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology of Neuronal Signals, Max Planck Institute for Experimental Medicine, 37075, Göttingen, Germany.

Show MeSH