Limits...
Developmental mapping of small-conductance calcium-activated potassium channel expression in the rat nervous system.

Gymnopoulos M, Cingolani LA, Pedarzani P, Stocker M - J. Comp. Neurol. (2014)

Bottom Line: The three SK channel subunits display different developmental expression gradients in distinct CNS regions, with time points of expression and up- or downregulation that can be associated with a range of diverse developmental events.Their early expression in embryonic development suggests an involvement of SK channels in the regulation of developmental processes.Additionally, this study shows how the postnatal ontogenetic patterns lead to the adult expression map for each SK channel subunit and how their coexpression in the same regions or neurons varies throughout development.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology of Neuronal Signals, Max Planck Institute for Experimental Medicine, 37075, Göttingen, Germany.

Show MeSH
SK subunit mRNA expression in rat embryos (E11: A–C; E12: D–F; E13: G–I; E15: J–L; and E17: M–O). X-ray film images of sections hybridized with oligonucleotide probes specific for SK1 (left column), SK2 (middle column) and SK3 (right column) show distribution of transcripts in the CNS and the body. Dark areas contain high levels of mRNA. For abbreviations see list. Abbreviations are according to Paxinos et al. (1994) and Paxinos and Watson (1998). Scale bar = 2 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4016743&req=5

fig02: SK subunit mRNA expression in rat embryos (E11: A–C; E12: D–F; E13: G–I; E15: J–L; and E17: M–O). X-ray film images of sections hybridized with oligonucleotide probes specific for SK1 (left column), SK2 (middle column) and SK3 (right column) show distribution of transcripts in the CNS and the body. Dark areas contain high levels of mRNA. For abbreviations see list. Abbreviations are according to Paxinos et al. (1994) and Paxinos and Watson (1998). Scale bar = 2 mm.

Mentions: Autoradiograms (see Figs. 2, 3, 7) provided an overview of the distribution of the three SK channel subunits in different areas of the developing rat brain. Analysis was performed on emulsion-dipped slides to resolve cellular labeling. All slides were emulsion coated, and the weak and strong signals in various brain regions shown in Tables6 were quantified according to the relative silver grain density on the individual cell bodies obtained with a given oligonucleotide. Expression levels were assessed by two independent observers. The scoring is the result of their joint assessment. The results of this analysis are presented in Tables6 as −, +, ++, or +++, with − representing signals below the threshold limit of detection and + a weak, ++ a moderate, and +++ the strongest level of expression. A scale of signal intensities with representative examples for each SK channel subunit is shown in Figure 3J–L. “Not clearly identifiable” (•) signifies that a given region could not be observed in our sections or could not be clearly differentiated from adjacent regions.


Developmental mapping of small-conductance calcium-activated potassium channel expression in the rat nervous system.

Gymnopoulos M, Cingolani LA, Pedarzani P, Stocker M - J. Comp. Neurol. (2014)

SK subunit mRNA expression in rat embryos (E11: A–C; E12: D–F; E13: G–I; E15: J–L; and E17: M–O). X-ray film images of sections hybridized with oligonucleotide probes specific for SK1 (left column), SK2 (middle column) and SK3 (right column) show distribution of transcripts in the CNS and the body. Dark areas contain high levels of mRNA. For abbreviations see list. Abbreviations are according to Paxinos et al. (1994) and Paxinos and Watson (1998). Scale bar = 2 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016743&req=5

fig02: SK subunit mRNA expression in rat embryos (E11: A–C; E12: D–F; E13: G–I; E15: J–L; and E17: M–O). X-ray film images of sections hybridized with oligonucleotide probes specific for SK1 (left column), SK2 (middle column) and SK3 (right column) show distribution of transcripts in the CNS and the body. Dark areas contain high levels of mRNA. For abbreviations see list. Abbreviations are according to Paxinos et al. (1994) and Paxinos and Watson (1998). Scale bar = 2 mm.
Mentions: Autoradiograms (see Figs. 2, 3, 7) provided an overview of the distribution of the three SK channel subunits in different areas of the developing rat brain. Analysis was performed on emulsion-dipped slides to resolve cellular labeling. All slides were emulsion coated, and the weak and strong signals in various brain regions shown in Tables6 were quantified according to the relative silver grain density on the individual cell bodies obtained with a given oligonucleotide. Expression levels were assessed by two independent observers. The scoring is the result of their joint assessment. The results of this analysis are presented in Tables6 as −, +, ++, or +++, with − representing signals below the threshold limit of detection and + a weak, ++ a moderate, and +++ the strongest level of expression. A scale of signal intensities with representative examples for each SK channel subunit is shown in Figure 3J–L. “Not clearly identifiable” (•) signifies that a given region could not be observed in our sections or could not be clearly differentiated from adjacent regions.

Bottom Line: The three SK channel subunits display different developmental expression gradients in distinct CNS regions, with time points of expression and up- or downregulation that can be associated with a range of diverse developmental events.Their early expression in embryonic development suggests an involvement of SK channels in the regulation of developmental processes.Additionally, this study shows how the postnatal ontogenetic patterns lead to the adult expression map for each SK channel subunit and how their coexpression in the same regions or neurons varies throughout development.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology of Neuronal Signals, Max Planck Institute for Experimental Medicine, 37075, Göttingen, Germany.

Show MeSH