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Developmental mapping of small-conductance calcium-activated potassium channel expression in the rat nervous system.

Gymnopoulos M, Cingolani LA, Pedarzani P, Stocker M - J. Comp. Neurol. (2014)

Bottom Line: The three SK channel subunits display different developmental expression gradients in distinct CNS regions, with time points of expression and up- or downregulation that can be associated with a range of diverse developmental events.Their early expression in embryonic development suggests an involvement of SK channels in the regulation of developmental processes.Additionally, this study shows how the postnatal ontogenetic patterns lead to the adult expression map for each SK channel subunit and how their coexpression in the same regions or neurons varies throughout development.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology of Neuronal Signals, Max Planck Institute for Experimental Medicine, 37075, Göttingen, Germany.

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Expression of SK1 and SK3 subunits in the postnatal cerebellum. SK1 shows strong expression in large neurons of the deep cerebellar nuclei (DN) at P12 (A). SK3 is also expressed at moderate levels in the deep nuclei at P12 (B). C–E: Darkfield photomicrographs displaying strong signal for the expression of SK3 transcripts in scattered Golgi cells at P6 (C), P12 (D), and P24 (E). Golgi cells are depicted at higher magnification in F, using brightfield optics that reveal clear clusters of silver grains on scattered cell nuclei (arrowheads). For abbreviations see list. For details on the distribution see Tables4 and 6. Scale bars = 300 μm in E (applies to A–E); 40 μm in F.
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fig14: Expression of SK1 and SK3 subunits in the postnatal cerebellum. SK1 shows strong expression in large neurons of the deep cerebellar nuclei (DN) at P12 (A). SK3 is also expressed at moderate levels in the deep nuclei at P12 (B). C–E: Darkfield photomicrographs displaying strong signal for the expression of SK3 transcripts in scattered Golgi cells at P6 (C), P12 (D), and P24 (E). Golgi cells are depicted at higher magnification in F, using brightfield optics that reveal clear clusters of silver grains on scattered cell nuclei (arrowheads). For abbreviations see list. For details on the distribution see Tables4 and 6. Scale bars = 300 μm in E (applies to A–E); 40 μm in F.

Mentions: To analyze the distribution patterns of SK transcripts after birth, we extended our in situ hybridization analysis and mapped the distributions of SK1, SK2, and SK3 mRNAs in the rat postnatal nervous system between P1 and P24 by examination of X-ray film images (Figs. 7) and emulsion-coated sections (Figs. 10, 12, 14, 15). The results of the evaluation at the cellular level are summarized in Tables6.


Developmental mapping of small-conductance calcium-activated potassium channel expression in the rat nervous system.

Gymnopoulos M, Cingolani LA, Pedarzani P, Stocker M - J. Comp. Neurol. (2014)

Expression of SK1 and SK3 subunits in the postnatal cerebellum. SK1 shows strong expression in large neurons of the deep cerebellar nuclei (DN) at P12 (A). SK3 is also expressed at moderate levels in the deep nuclei at P12 (B). C–E: Darkfield photomicrographs displaying strong signal for the expression of SK3 transcripts in scattered Golgi cells at P6 (C), P12 (D), and P24 (E). Golgi cells are depicted at higher magnification in F, using brightfield optics that reveal clear clusters of silver grains on scattered cell nuclei (arrowheads). For abbreviations see list. For details on the distribution see Tables4 and 6. Scale bars = 300 μm in E (applies to A–E); 40 μm in F.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016743&req=5

fig14: Expression of SK1 and SK3 subunits in the postnatal cerebellum. SK1 shows strong expression in large neurons of the deep cerebellar nuclei (DN) at P12 (A). SK3 is also expressed at moderate levels in the deep nuclei at P12 (B). C–E: Darkfield photomicrographs displaying strong signal for the expression of SK3 transcripts in scattered Golgi cells at P6 (C), P12 (D), and P24 (E). Golgi cells are depicted at higher magnification in F, using brightfield optics that reveal clear clusters of silver grains on scattered cell nuclei (arrowheads). For abbreviations see list. For details on the distribution see Tables4 and 6. Scale bars = 300 μm in E (applies to A–E); 40 μm in F.
Mentions: To analyze the distribution patterns of SK transcripts after birth, we extended our in situ hybridization analysis and mapped the distributions of SK1, SK2, and SK3 mRNAs in the rat postnatal nervous system between P1 and P24 by examination of X-ray film images (Figs. 7) and emulsion-coated sections (Figs. 10, 12, 14, 15). The results of the evaluation at the cellular level are summarized in Tables6.

Bottom Line: The three SK channel subunits display different developmental expression gradients in distinct CNS regions, with time points of expression and up- or downregulation that can be associated with a range of diverse developmental events.Their early expression in embryonic development suggests an involvement of SK channels in the regulation of developmental processes.Additionally, this study shows how the postnatal ontogenetic patterns lead to the adult expression map for each SK channel subunit and how their coexpression in the same regions or neurons varies throughout development.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology of Neuronal Signals, Max Planck Institute for Experimental Medicine, 37075, Göttingen, Germany.

Show MeSH