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Developmental mapping of small-conductance calcium-activated potassium channel expression in the rat nervous system.

Gymnopoulos M, Cingolani LA, Pedarzani P, Stocker M - J. Comp. Neurol. (2014)

Bottom Line: The three SK channel subunits display different developmental expression gradients in distinct CNS regions, with time points of expression and up- or downregulation that can be associated with a range of diverse developmental events.Their early expression in embryonic development suggests an involvement of SK channels in the regulation of developmental processes.Additionally, this study shows how the postnatal ontogenetic patterns lead to the adult expression map for each SK channel subunit and how their coexpression in the same regions or neurons varies throughout development.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology of Neuronal Signals, Max Planck Institute for Experimental Medicine, 37075, Göttingen, Germany.

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Laminar distribution of SK1, SK2, and SK3 transcripts in the neocortex during postnatal development. SK1 (A,D,G,J,M) and SK2 (B,E,H,K,N) display a similar developmental pattern, starting with relatively strong expression in multiple layers at P1–P3 and leading to signals that are predominant in layer V (V) neurons at P24, but SK3 (C,F,I,L,O) shows a progressive decline in expression in all layers, with a moderate level persisting in the deep part of layer VI (VI) at P24. For abbreviations see list. For details on the distribution see Tables6. Scale bar = 400 μm.
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fig10: Laminar distribution of SK1, SK2, and SK3 transcripts in the neocortex during postnatal development. SK1 (A,D,G,J,M) and SK2 (B,E,H,K,N) display a similar developmental pattern, starting with relatively strong expression in multiple layers at P1–P3 and leading to signals that are predominant in layer V (V) neurons at P24, but SK3 (C,F,I,L,O) shows a progressive decline in expression in all layers, with a moderate level persisting in the deep part of layer VI (VI) at P24. For abbreviations see list. For details on the distribution see Tables6. Scale bar = 400 μm.

Mentions: To analyze the distribution patterns of SK transcripts after birth, we extended our in situ hybridization analysis and mapped the distributions of SK1, SK2, and SK3 mRNAs in the rat postnatal nervous system between P1 and P24 by examination of X-ray film images (Figs. 7) and emulsion-coated sections (Figs. 10, 12, 14, 15). The results of the evaluation at the cellular level are summarized in Tables6.


Developmental mapping of small-conductance calcium-activated potassium channel expression in the rat nervous system.

Gymnopoulos M, Cingolani LA, Pedarzani P, Stocker M - J. Comp. Neurol. (2014)

Laminar distribution of SK1, SK2, and SK3 transcripts in the neocortex during postnatal development. SK1 (A,D,G,J,M) and SK2 (B,E,H,K,N) display a similar developmental pattern, starting with relatively strong expression in multiple layers at P1–P3 and leading to signals that are predominant in layer V (V) neurons at P24, but SK3 (C,F,I,L,O) shows a progressive decline in expression in all layers, with a moderate level persisting in the deep part of layer VI (VI) at P24. For abbreviations see list. For details on the distribution see Tables6. Scale bar = 400 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016743&req=5

fig10: Laminar distribution of SK1, SK2, and SK3 transcripts in the neocortex during postnatal development. SK1 (A,D,G,J,M) and SK2 (B,E,H,K,N) display a similar developmental pattern, starting with relatively strong expression in multiple layers at P1–P3 and leading to signals that are predominant in layer V (V) neurons at P24, but SK3 (C,F,I,L,O) shows a progressive decline in expression in all layers, with a moderate level persisting in the deep part of layer VI (VI) at P24. For abbreviations see list. For details on the distribution see Tables6. Scale bar = 400 μm.
Mentions: To analyze the distribution patterns of SK transcripts after birth, we extended our in situ hybridization analysis and mapped the distributions of SK1, SK2, and SK3 mRNAs in the rat postnatal nervous system between P1 and P24 by examination of X-ray film images (Figs. 7) and emulsion-coated sections (Figs. 10, 12, 14, 15). The results of the evaluation at the cellular level are summarized in Tables6.

Bottom Line: The three SK channel subunits display different developmental expression gradients in distinct CNS regions, with time points of expression and up- or downregulation that can be associated with a range of diverse developmental events.Their early expression in embryonic development suggests an involvement of SK channels in the regulation of developmental processes.Additionally, this study shows how the postnatal ontogenetic patterns lead to the adult expression map for each SK channel subunit and how their coexpression in the same regions or neurons varies throughout development.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology of Neuronal Signals, Max Planck Institute for Experimental Medicine, 37075, Göttingen, Germany.

Show MeSH