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Copper-transporting ATPase is important for malaria parasite fertility.

Kenthirapalan S, Waters AP, Matuschewski K, Kooij TW - Mol. Microbiol. (2013)

Bottom Line: We show that male and female gametocytes are abundant in cutp(-) parasites, but activation of male microgametes and exflagellation were strongly impaired.A cross-fertilization assay demonstrated that female fertility was also severely abrogated.In conclusion, we provide experimental genetic and pharmacological evidence that a healthy copper homeostasis is critical to malaria parasite fertility of both genders of gametocyte and, hence, to transmission to the mosquito vector.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Infection Biology, Parasitology Unit, 10117, Berlin, Germany.

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Reduction of male microgamete exflagellation in the presence of the cell-permeable Cu+-chelator neocuproine.Shown are the numbers of exflagellating WT and cutp− male gametocytes in the presence or absence of 1 μM neocuproine (*P < 0.05; non-parametric, two-tailed Mann–Whitney's test).
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fig06: Reduction of male microgamete exflagellation in the presence of the cell-permeable Cu+-chelator neocuproine.Shown are the numbers of exflagellating WT and cutp− male gametocytes in the presence or absence of 1 μM neocuproine (*P < 0.05; non-parametric, two-tailed Mann–Whitney's test).

Mentions: To further substantiate the link between male fertility and copper, we investigated the effects of copper ion addition and chelation on exflagellation in an in vitro assay. Chelation of intracellular Cu+ with neocuproine in WT parasites resulted in a substantial reduction of male exflagellation, mimicking the defect observed in cutp− parasites (Fig. 6). In contrast, we were not able to restore the exflagellation deficit of the cutp− parasites or neocuproine-treated WT parasites by addition of extracellular Cu+ or Cu2+ (data not shown).


Copper-transporting ATPase is important for malaria parasite fertility.

Kenthirapalan S, Waters AP, Matuschewski K, Kooij TW - Mol. Microbiol. (2013)

Reduction of male microgamete exflagellation in the presence of the cell-permeable Cu+-chelator neocuproine.Shown are the numbers of exflagellating WT and cutp− male gametocytes in the presence or absence of 1 μM neocuproine (*P < 0.05; non-parametric, two-tailed Mann–Whitney's test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016742&req=5

fig06: Reduction of male microgamete exflagellation in the presence of the cell-permeable Cu+-chelator neocuproine.Shown are the numbers of exflagellating WT and cutp− male gametocytes in the presence or absence of 1 μM neocuproine (*P < 0.05; non-parametric, two-tailed Mann–Whitney's test).
Mentions: To further substantiate the link between male fertility and copper, we investigated the effects of copper ion addition and chelation on exflagellation in an in vitro assay. Chelation of intracellular Cu+ with neocuproine in WT parasites resulted in a substantial reduction of male exflagellation, mimicking the defect observed in cutp− parasites (Fig. 6). In contrast, we were not able to restore the exflagellation deficit of the cutp− parasites or neocuproine-treated WT parasites by addition of extracellular Cu+ or Cu2+ (data not shown).

Bottom Line: We show that male and female gametocytes are abundant in cutp(-) parasites, but activation of male microgametes and exflagellation were strongly impaired.A cross-fertilization assay demonstrated that female fertility was also severely abrogated.In conclusion, we provide experimental genetic and pharmacological evidence that a healthy copper homeostasis is critical to malaria parasite fertility of both genders of gametocyte and, hence, to transmission to the mosquito vector.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Infection Biology, Parasitology Unit, 10117, Berlin, Germany.

Show MeSH
Related in: MedlinePlus