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Polymorphism of the cytochrome P-450 1A1 (A2455G) in women with breast cancer in Eastern Azerbaijan, Iran.

Saadatian H, Gharesouran J, Montazeri V, Mohammadi SA, Mohaddes Ardabili SM - Iran J Basic Med Sci (2014)

Bottom Line: Cytochrome P-450 1A1 is an important enzyme in the first phase of the metabolism of some carcinogens such as polycyclic aromatic hydrocarbons (PAHs), as well as estrogen.Higher frequency of heterozygotes was mainly observed among premenopausal breast cancer patients.Our results suggest that the CYP1A1 polymorphism may be useful for predicting breast cancer risk in our study population.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

ABSTRACT

Objective(s): Cytochrome P-450 1A1 is an important enzyme in the first phase of the metabolism of some carcinogens such as polycyclic aromatic hydrocarbons (PAHs), as well as estrogen. The present study evaluates the existence of CYP1A1 polymorphism in a number of breast cancer samples.

Materials and methods: One hundred breast cancer patients and the same number of healthy controls were analyzed for the A2455G polymorphism of cytochrome P-450 1A1 by the polymerase chain reaction-restriction fragment length polymorphism technique.

Results: Frequency of heterozygote genotype (A/G) indicated significant increase in case group (17%) compared to control group (7%) (OR=2.7; 95% CI=1.07-6.89; P-value=0.03). The related result of (A/A) genotype showed significantly decreased risk of breast cancer (OR=0.34; P-value=0.01). Higher frequency of heterozygotes was mainly observed among premenopausal breast cancer patients.

Conclusion: Our results suggest that the CYP1A1 polymorphism may be useful for predicting breast cancer risk in our study population.

No MeSH data available.


Related in: MedlinePlus

Polymerase chain reaction-restriction fragment length polymorphism agarose gel electrophoresis of cytochrome1A1 (A2455G). Lane 1 referred to heterozygote genotype and the rest of the samples, except for lane 6 that is 50 bp marker, are indicative of wild homozygote
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Figure 1: Polymerase chain reaction-restriction fragment length polymorphism agarose gel electrophoresis of cytochrome1A1 (A2455G). Lane 1 referred to heterozygote genotype and the rest of the samples, except for lane 6 that is 50 bp marker, are indicative of wild homozygote

Mentions: In the present study allele frequencies of the variant allele CYP1A1 (A2455G) among the cases and controls were 0.1 and 0.04, respectively. The genotype frequencies and the results of the case control study have been summarized in Table 2. The wild and variant alleles have been designated as A and G respectively. As it can be concluded from Table 2, the risk of breast cancer was increased among heterozygote women carrying the variant allele G (462Val) (odds ratio 2.70, 95% CI 1.075-6.89, P-value=0.03. The risk appeared to be increased predominantly in pre-menopausal subgroup (odds ratio 11.66, 95% CI 1.5 -90.76, P-value=0.004. The related result of genotype assessment showed significantly decreased risk of breast cancer for homozyghote wild genotype (A/A) (OR=0.34; P-value=0.01). Genotype distribution in both case and control groups did not diverge significantly from Hardy-Weinberg equilibrium. In addition, we found no significant discrepancy among (A/G) genotype and clinic-pathological characteristics regarding the tumor size and grade. According to lymph node status, the frequency of heterozygote genotype was slightly increased in positive lymph nodes. Figure 1 shows the PCR-RFLP agarose gel electrophoresis of CYP1A1 (A2455G) gene.


Polymorphism of the cytochrome P-450 1A1 (A2455G) in women with breast cancer in Eastern Azerbaijan, Iran.

Saadatian H, Gharesouran J, Montazeri V, Mohammadi SA, Mohaddes Ardabili SM - Iran J Basic Med Sci (2014)

Polymerase chain reaction-restriction fragment length polymorphism agarose gel electrophoresis of cytochrome1A1 (A2455G). Lane 1 referred to heterozygote genotype and the rest of the samples, except for lane 6 that is 50 bp marker, are indicative of wild homozygote
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016695&req=5

Figure 1: Polymerase chain reaction-restriction fragment length polymorphism agarose gel electrophoresis of cytochrome1A1 (A2455G). Lane 1 referred to heterozygote genotype and the rest of the samples, except for lane 6 that is 50 bp marker, are indicative of wild homozygote
Mentions: In the present study allele frequencies of the variant allele CYP1A1 (A2455G) among the cases and controls were 0.1 and 0.04, respectively. The genotype frequencies and the results of the case control study have been summarized in Table 2. The wild and variant alleles have been designated as A and G respectively. As it can be concluded from Table 2, the risk of breast cancer was increased among heterozygote women carrying the variant allele G (462Val) (odds ratio 2.70, 95% CI 1.075-6.89, P-value=0.03. The risk appeared to be increased predominantly in pre-menopausal subgroup (odds ratio 11.66, 95% CI 1.5 -90.76, P-value=0.004. The related result of genotype assessment showed significantly decreased risk of breast cancer for homozyghote wild genotype (A/A) (OR=0.34; P-value=0.01). Genotype distribution in both case and control groups did not diverge significantly from Hardy-Weinberg equilibrium. In addition, we found no significant discrepancy among (A/G) genotype and clinic-pathological characteristics regarding the tumor size and grade. According to lymph node status, the frequency of heterozygote genotype was slightly increased in positive lymph nodes. Figure 1 shows the PCR-RFLP agarose gel electrophoresis of CYP1A1 (A2455G) gene.

Bottom Line: Cytochrome P-450 1A1 is an important enzyme in the first phase of the metabolism of some carcinogens such as polycyclic aromatic hydrocarbons (PAHs), as well as estrogen.Higher frequency of heterozygotes was mainly observed among premenopausal breast cancer patients.Our results suggest that the CYP1A1 polymorphism may be useful for predicting breast cancer risk in our study population.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

ABSTRACT

Objective(s): Cytochrome P-450 1A1 is an important enzyme in the first phase of the metabolism of some carcinogens such as polycyclic aromatic hydrocarbons (PAHs), as well as estrogen. The present study evaluates the existence of CYP1A1 polymorphism in a number of breast cancer samples.

Materials and methods: One hundred breast cancer patients and the same number of healthy controls were analyzed for the A2455G polymorphism of cytochrome P-450 1A1 by the polymerase chain reaction-restriction fragment length polymorphism technique.

Results: Frequency of heterozygote genotype (A/G) indicated significant increase in case group (17%) compared to control group (7%) (OR=2.7; 95% CI=1.07-6.89; P-value=0.03). The related result of (A/A) genotype showed significantly decreased risk of breast cancer (OR=0.34; P-value=0.01). Higher frequency of heterozygotes was mainly observed among premenopausal breast cancer patients.

Conclusion: Our results suggest that the CYP1A1 polymorphism may be useful for predicting breast cancer risk in our study population.

No MeSH data available.


Related in: MedlinePlus