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Study of antagonistic effects of Lactobacillus strains as probiotics on multi drug resistant (MDR) bacteria isolated from urinary tract infections (UTIs).

Naderi A, Kasra-Kermanshahi R, Gharavi S, Imani Fooladi AA, Abdollahpour Alitappeh M, Saffarian P - Iran J Basic Med Sci (2014)

Bottom Line: However, an inhibitory effect was observed for E. coli which were resistant to 8-9 antibiotics.In addition, L. casei was determined to be the most effective probiotic.Treatment of E. coli with probiotic suspension was not effective on inhibition of the plasmid carrying hypothetical ampicillin resistant gene.

View Article: PubMed Central - PubMed

Affiliation: Division of Microbiology, Department of Biology, Faculty of Sciences, Alzahra University, Tehran, Iran.

ABSTRACT

Objective(s): Urinary tract infection (UTI) caused by bacteria is one of the most frequent infections in human population. Inappropriate use of antibiotics, often leads to appearance of drug resistance in bacteria. However, use of probiotic bacteria has been suggested as a partial replacement. This study was aimed to assess the antagonistic effects of Lactobacillus standard strains against bacteria isolated from UTI infections.

Materials and methods: Among 600 samples; those with ≥10,000 cfu/ml were selected as UTI positive samples. Enterococcus sp., Klebsiella pneumoniae, Enterobacter sp., and Escherichia coli were found the most prevalent UTI causative agents. All isolates were screened for multi drug resistance and subjected to the antimicrobial effects of three Lactobacillus strains by using microplate technique and the MICs amounts were determined. In order to verify the origin of antibiotic resistance of isolates, plasmid curing using ethidium bromide and acridine orange was carried out.

Results: No antagonistic activity in Lactobacilli suspension was detected against test on Enterococcus and Enterobacter strains and K. pneumoniae, which were resistant to most antibiotics. However, an inhibitory effect was observed for E. coli which were resistant to 8-9 antibiotics. In addition, L. casei was determined to be the most effective probiotic. RESULTS from replica plating suggested one of the plasmids could be related to the gene responsible for ampicillin resistance.

Conclusion: Treatment of E. coli with probiotic suspension was not effective on inhibition of the plasmid carrying hypothetical ampicillin resistant gene. Moreover, the plasmid profiles obtained from probiotic-treated isolates were identical to untreated isolates.

No MeSH data available.


Related in: MedlinePlus

Agarose gel electrophoresis showing plasmid pattern of strain number 6. Lane 1: treated strain with L. casei supernatant; Lane 2: treated strain with L. acidophilus supernatant; Lane 3: treated strain with L. rhamnosus supernatant
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Figure 10: Agarose gel electrophoresis showing plasmid pattern of strain number 6. Lane 1: treated strain with L. casei supernatant; Lane 2: treated strain with L. acidophilus supernatant; Lane 3: treated strain with L. rhamnosus supernatant

Mentions: After incubation of test bacteria in the LB media containing sub-MIC concentrations of each lactobacillus culture supernatants, isolation of mutant strains were carried out using replica plating method and no mutant was identified. Moreover, plasmid extraction from treated strains was performed and plasmid patterns of each sample were determined; however, no difference was observed between plasmid patterns of treated strains with that of maternal strain (Figure 10).


Study of antagonistic effects of Lactobacillus strains as probiotics on multi drug resistant (MDR) bacteria isolated from urinary tract infections (UTIs).

Naderi A, Kasra-Kermanshahi R, Gharavi S, Imani Fooladi AA, Abdollahpour Alitappeh M, Saffarian P - Iran J Basic Med Sci (2014)

Agarose gel electrophoresis showing plasmid pattern of strain number 6. Lane 1: treated strain with L. casei supernatant; Lane 2: treated strain with L. acidophilus supernatant; Lane 3: treated strain with L. rhamnosus supernatant
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016691&req=5

Figure 10: Agarose gel electrophoresis showing plasmid pattern of strain number 6. Lane 1: treated strain with L. casei supernatant; Lane 2: treated strain with L. acidophilus supernatant; Lane 3: treated strain with L. rhamnosus supernatant
Mentions: After incubation of test bacteria in the LB media containing sub-MIC concentrations of each lactobacillus culture supernatants, isolation of mutant strains were carried out using replica plating method and no mutant was identified. Moreover, plasmid extraction from treated strains was performed and plasmid patterns of each sample were determined; however, no difference was observed between plasmid patterns of treated strains with that of maternal strain (Figure 10).

Bottom Line: However, an inhibitory effect was observed for E. coli which were resistant to 8-9 antibiotics.In addition, L. casei was determined to be the most effective probiotic.Treatment of E. coli with probiotic suspension was not effective on inhibition of the plasmid carrying hypothetical ampicillin resistant gene.

View Article: PubMed Central - PubMed

Affiliation: Division of Microbiology, Department of Biology, Faculty of Sciences, Alzahra University, Tehran, Iran.

ABSTRACT

Objective(s): Urinary tract infection (UTI) caused by bacteria is one of the most frequent infections in human population. Inappropriate use of antibiotics, often leads to appearance of drug resistance in bacteria. However, use of probiotic bacteria has been suggested as a partial replacement. This study was aimed to assess the antagonistic effects of Lactobacillus standard strains against bacteria isolated from UTI infections.

Materials and methods: Among 600 samples; those with ≥10,000 cfu/ml were selected as UTI positive samples. Enterococcus sp., Klebsiella pneumoniae, Enterobacter sp., and Escherichia coli were found the most prevalent UTI causative agents. All isolates were screened for multi drug resistance and subjected to the antimicrobial effects of three Lactobacillus strains by using microplate technique and the MICs amounts were determined. In order to verify the origin of antibiotic resistance of isolates, plasmid curing using ethidium bromide and acridine orange was carried out.

Results: No antagonistic activity in Lactobacilli suspension was detected against test on Enterococcus and Enterobacter strains and K. pneumoniae, which were resistant to most antibiotics. However, an inhibitory effect was observed for E. coli which were resistant to 8-9 antibiotics. In addition, L. casei was determined to be the most effective probiotic. RESULTS from replica plating suggested one of the plasmids could be related to the gene responsible for ampicillin resistance.

Conclusion: Treatment of E. coli with probiotic suspension was not effective on inhibition of the plasmid carrying hypothetical ampicillin resistant gene. Moreover, the plasmid profiles obtained from probiotic-treated isolates were identical to untreated isolates.

No MeSH data available.


Related in: MedlinePlus